Leica CM1510, Instruction Manual

Page: 22 / 32

22 Instruction Manual V2.1 – 12/2001
9. Trouble shooting
Problem Causes Remedies
Change place of installation for
the cryostat.
- Wear mouth protection.
- Select lower temperature.
- Wait until knife/blade and/or anti-
roll plate have reached chamber
temperature.
- Select higher temperature.
- Remove cause.
- Select lower temperature.
- Trim the specimen parallel, in-
crease section thickness.
- Reposition anti-roll plate.
- Align correctly.
- Set correct angle.
- Use different part of the cutting
edge or replace the knife/blade.
- Clean with dry cloth or brush.
- Replace plate.
- Use different part of the cutting
edge or replace the knife/blade.
- Readjust correctly.
- Readjust correctly.
- Use different part of the cutting
edge or replace the knife/blade.
- Replace the plate.
- Cryostat is exposed to air cur-
rents (open windows and doors,
air conditioning).
- Sliding window was open and
exposed to air currents too long.
- Frost built up by breathing into the
cryochamber.
- Specimen not cold enough.
- Knife/blade and/or anti-roll plate
not yet cold enough and thus
warm the sections.
- Specimen too cold
- Static electricity/air currents.
- Specimen not cold enough.
- Large area specimen.
- Anti-roll plate poorly positioned.
- Anti-roll plate poorly aligned with
cutting edge.
- Incorrect clearance angle.
- Cutting edge blunt or damaged.
- Knife/blade and/or anti-roll
plate dirty.
- Edge of anti-roll plate damaged.
- Blunt cutting edge.
- Anti-roll plate does not protrude
far enough beyond the cutting
edge.
- Anti-roll plate protrudes too far
beyond the knife edge and is
scraping against the specimen.
- Cutting edge damaged.
- Edge of anti-roll plate damaged.
Frost on chamber walls and
microtome
Sections smear
Sections splinter
Sections not properly flattened
Sections not properly flattened
despite correct temperature and
correctly aligned anti-roll plate
Sections curl on the anti-roll plate
Scraping noise during sectioning
and specimen return movement
Ridged sections