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Leica CM1510 – Cryostat

Cleaning and disinfection

• Do not use organic solvents or any other

aggressive substances for cleaning and
disinfection!

We strongly recommend the use of Leica

Cryofect disinfectant spray! Only use al-
cohol or common disinfectants based on
alcohol!

• For spray disinfection follow the instruc-

tions for use supplied with the disinfec-
tant!

• Do not use external heaters for drying the

cryochamber. This can cause severe dam-
age to the cooling system!

Removal of the microtome

• Before removing the microtome, turn the

instrument off with the mains switch and
disconnect it from mains!

• Before taking the microtome out of the

cryochamber, place the handle of the
handwheel in the lowest position.

When removing the microtome, the speci-

men head will rapidly fall down and might
injure the operator‘s hands!

• Wear appropriate protective gloves to re-

move the cold microtome from the cryo-
chamber. Risk of frost bite!

• The microtome must be completely dry be-

fore reinstallation. Humidity inside will
condense and freeze in the cold cryostat
and thus may cause malfunctions or dam-
age.

Safety instructions for handling the instrument

Maintenance

• Only qualified and authorized service per-

sonnel may access the internal compo-
nents of the instrument for service and re-
pair.

• Clean the compressor ventilation grid at

least once a year or more often if required!

Fuse replacement (Chapter 11)

• Turn the instrument off with the mains

switch and pull the mains plug, before re-
placing the fuses.

•

Only use fuses of the same specification!

For the required values, please refer to
Chapter 4 'Technical data'.

Lamp replacement (Chapter 11)

• Turn the instrument off with the mains

switch and pull the mains plug, before re-
placing the lamp.

• If the lamp is broken, it must be replaced

by the technical service, as the replace-
ment involves a high risk of injury.

• Do not use lamp type other than specified

by the manufacturer!

Summary of Contents for CM1510

Page 1: ...Instruction Manual Leica CM1510 V2 1 English 12 2001 Always keep this manual near the instrument Read carefully prior to operating the instrument Leica CM1510 Cryostat ...

Page 2: ......

Page 3: ...ing to the national legal system applicable in each individual case In par ticular no liability whatsoever is accepted for any fi nancial loss or consequential damage caused by or related to compliance with statements or other infor mation in this manual Statements drawings illustrations and other informa tion as regards contents or technical details of the present manual are not to be considered ...

Page 4: ...ration 17 8 1 Specimen freezing 17 8 2 Mounting of specimen discs 18 8 3 Inserting the knife or blade in the knife holder 19 8 4 Moving the specimen towards or away from the knife blade via the coarse feed 19 8 5 Trimming 19 8 6 Adjusting the anti roll guide 20 8 7 Sectioning and section transfer 20 8 8 Defrosting 21 9 Trouble shooting 22 10 Cleaning and disinfection 25 10 1 Cleaning 25 10 2 Spray...

Page 5: ... the handle 1 is posi tioned in the upper turning point To lock push the lever 2 to the right To unlock push the lever 2 to the left 3 Safety instructions for handling the instrument 3 1 2 Knife guard Prior to manipulating the knife and specimen or during breaks always cover the knife edge with the knife guard The knife holder has a knife guard which is positioned over the knife edge to prevent in...

Page 6: ...t be set up with at least 10 cm distance from walls and furniture Do not place anything next to the compres sor ventilation grid right side of the cabi net to ensure adequate ventilation Remove the protective foam parts for the microtome and for the evaporator prior to operating the instrument see Fig 13 2 Connection to mains power Please refer to the Technical data The instrument must be connecte...

Page 7: ...to re move the cold microtome from the cryo chamber Risk of frost bite The microtome must be completely dry be fore reinstallation Humidity inside will condense and freeze in the cold cryostat and thus may cause malfunctions or dam age 3 Safety instructions for handling the instrument Maintenance Only qualified and authorized service per sonnel may access the internal compo nents of the instrument...

Page 8: ...tion 8 min Power draw 850 W 495 W Refrigerating output at an evaporation temperature of 25 C 780 W 585 W Nominal pressure 25 bar maximum value Safety factor 3 Refrigerant 240 g 5 g R404A Compressor oil 0 6 l EMKARATE RL244b ICI Microtome Type Rotary microtome Section thickness setting 1 50 µm in 1 µm increments from 1 to 10 µm in 2 µm increments from 10 to 20 µm in 5 µm increments from 20 to 50 µm...

Page 9: ...mal block optional accessory 5 1 Leica CM 1510 and components 5 General description Fig 9 Waste tray Brush shelf Heat extractor optional accessory Quick freeze shelf Knife holder CE Knife holder CN Knife holder base Specimen disc ...

Page 10: ...s a compact open top cryostat in a space savingdesignforrapidfreezingandmanualsec tioning of tissue specimens The cabinet is movable on rollers Two adjustable feet 4 at the front ensure a safe stand The counterbal anced handwheel 2 is lockable in the top position The corrosion proof cryochamber is well illuminated and easily accessible through the heated sliding win dow The cryochamber temperature...

Page 11: ...to level the instrument Do not operate in rooms with explosion haz ard To ensure an adequate cooling capacity the instrument has to be set up with at least 10 cm distance from walls and furniture Do not place anything next to the compressor ventilation grid right side of the cabinet to ensure adequate ventilation The place of installation must meet the following re quirements No direct sunlight Ma...

Page 12: ...n the cardboard box for the accessories Insert the pin 1 of the handwheel shaft in to the hole 2 of the handwheel Mount the spring washer 3 on the screw 4 as shown in Fig 12 1 Tighten the screw 4 with an Allen key 5 mm To disassemble proceed in reverse order Remove the protective foam parts for the mi crotome and for the evaporator 1 prior to op erating the instrument see Fig 12 2 ...

Page 13: ... tric circuit 2 1 Fig 13 Before connecting the instrument to the mains power pleasecheckifthelocalmainsvoltagecom plies with the power rating indicated on the name plate of the instrument Place the storage shelves in the cryochamber Place the waste tray 1 and brush shelf 2 in the cryochamber Place the knife holder base on the microtome base plate Place the knife holder on the knife holder base and...

Page 14: ...ter turning on it will take approximately 5 seconds until the compressor starts operat ing Electric coarse feed forward backward ON OFF switch for chamber illumination Display for set and actual values Control unit Fig 14 2 Chamber illumination Electric coarse feed The electric coarse feed provides a rapid movement of the specimen towards or away from the knife The specimen movement towards the cu...

Page 15: ...ion the last digit is used for minute indication Sincethereisjustonedigitfortheminute indication the shown value has to be multiplied by ten The defrost time can be adjusted in 10 minute steps Fig 15 2 shows defrosting time set at 23 50 hours To set the real time press P for hour indication and modify the value via arrow key Press P again for minute indication and modify the value via ar row key W...

Page 16: ...ittle fat Cartilage Cervix Fat Heart and vessel Intestine Kidney Laryngeal Lip Liver Lung Lymphoid Muscular Nose Pancreas Prostate Ovary Rectal Skin with fat Skin without fat Spleen or bloody tissue Testicular Thyroid Tongue Uterus curettings Tissue 10 15 15 25 25 30 Above temperature values are based on long term experience but are only approximate values ...

Page 17: ...cimen to size The specimen should be no greater than 2 cm x 2 cm x 2 cm Use a specimen disc at room temperature Apply enough cryocompound to the specimen disc Place the specimen on the disc and orient Remove the cover from the quick freeze shelf Place the specimen disc with the specimen in one of the holes of the quick freeze shelf and freeze the specimen Specimen freezing can be accelerated by ad...

Page 18: ...lace cover the knife edge with the knife guard Loosen the screw 1 on the specimen head Insert the shaft of the specimen disc 3 with the frozenspecimeninthelocationhole 2 ofthespeci men head Make sure that the shaft of the specimen disc is fully inserted The entire rear surface must have a good contact with the specimen head Retighten screw 1 8 2 3 Specimen orientation To release loosen screw 4 Ori...

Page 19: ...cutting edge is extremely sharp and can cause severe injury Never leave knives blades or knife holders with a knife blade mounted lying around Do not place a knife on a table with the cut ting edge facing upward Never try to catch a falling knife Always clamp the specimen before the knife Prior to manipulating the knife and specimen or changing the specimen or knife and dur ing breaks always lock ...

Page 20: ...cut section The frozen section flies to the slide rapidly thaws and thus adheres to the slide surface making subsequent orientation impossible Section transfer to a precooled slide Carefully apply the section to the slide by gentle brushing Orient and flatten the section on the slide surface by means of the brush To ensure the section tightly adheres to the slide surface warmthecoldslideplacingafi...

Page 21: ...es in the factory After defrosting cooling is resumed automatically The cryochamber will then be cooled to the previously se lected set temperature During defrosting the actual chamber tem perature is displayed and LED 2 is illuminated to indicate that a defrost cycle takes place The temperature of the quick freeze shelf is always lower than that of the cryochamber which may result in increased fr...

Page 22: ...sed to air currents too long Frostbuiltupbybreathingintothe cryochamber Specimen not cold enough Knife blade and or anti roll plate not yet cold enough and thus warm the sections Specimen too cold Static electricity air currents Specimen not cold enough Large area specimen Anti roll plate poorly positioned Anti roll plate poorly aligned with cutting edge Incorrect clearance angle Cutting edge blun...

Page 23: ...n the knife back Handwheel speed not uni form Knife blade not clamped tightly enough Specimen holder not clamped tightly Cryocompound applied to cold specimen disc specimen detached from the disc after freezing Blunt cutting edge Incorrect clearance angle Microtome not properly dried before reinstallation Refreeze specimen onto the disc Check disc clamping Check ball joint clamping Check clamping ...

Page 24: ...n consequently ice built up in the micrometer feed system Defective microtome Moisture on the underside caused the disc to freeze to the quick freeze shelf or specimen head Mains plug not properly con nected Defective fuses Stopper not inserted in the drain hole of the chamber Room temperature too high Compressor defective Air humidity and room tempera ture too high Cool down anti roll plate or re...

Page 25: ...inated surfaces the surfaces should be covered with an even layer or 5b soak a cloth with disinfectant and apply it on the contaminated surfaces 6 Allow a reaction time of no less than 15 minutes 7 Wipe it off with a tissue 8 Dispose of tissue in compliance with the ruling waste disposal regulations of your institution 9 Set the cryochamber temperature to the originally selected value If increased...

Page 26: ...rse feed motor plug 2 and the temperature sensor 1 Upon removing the brush shelf two screws 4 be come visible which should be loosened with a 5 mm Allen key Pull the stopper at the bottom of the cryochamber to drain any condensate waste or cleaning liquid that may have collected in the cryochamber Replace the stopper Dispose of the waste liquid according to the local waste disposal regulations All...

Page 27: ...the cryochamber 10 4 Reassembly of the microtome The microtome must be completely dry before reinstallation Humidity inside will condense and freeze in the cold cryostat and thus may cause malfunctions or damage Replace the microtome in the cryochamber Mount the plastic coupling 5 on the handwheel shaft Rotate the handle of the handwheel to the lowest position and hold it specimen clamping head is...

Page 28: ...ation to a great extent Toensurereliableandtrouble freeoperationoversev eral years we recommend the following Have the instrument inspected by a qualified ser vice engineer authorized by Leica at least once a year Enter into a service contract after the warranty pe riod for further details please contact your Leica sales office Clean the instrument every day From time to time Apply adrop of cryost...

Page 29: ...p for better orientation Slightly tilt the fluorescent tube down to the left and pull it out of the clip 5 Hold the lamp with both hands and pull it to the left out of the lamp holder 6 Do not use lamp type other than specified by the manufacturer Assembly of the new lamp Lamp type 230 V 50 Hz OSRAM DULUX S 11 W 115 V 60 Hz OSRAM DULUX S 13 W Hold the lamp in the correct mounting position as shown...

Page 30: ...mber 12 1 2Stat ionary heat extractor Screw the heat extractor into the hole on the quick freeze shelf It can reach three specimen discs Lower the heat extractor cylinder on the tissue sur face Afterapproximately30seconds thespecimen will be entirely frozen To prevent unnecessary distortion of the specimen during contact freezing the weight of the heat extractor can be balanced To balance the weig...

Page 31: ... be considered as inspected and or approved by Leica Installation or use of any such parts may impair the technical design features and thus the properties of the instrument Warranty claims can only be made as long as the prod uct or system has been operated according to its des ignated use and according to the instructions given in this manual 13 Appendix In case of justified claims the manufactu...

Page 32: ...32 Instruction Manual V2 1 12 2001 Notes ...

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