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© 2018 Corning Incorporated

28

Finishing the centrifuging

ENDING CENTRIFUGING

When preselected time is reached, centrifugation will end automatically.

X1

Before lapse preselected time one may stop centrifugation. Pressing STOP for the

first time will stop centrifuging with the charasteristic set in loaded program. Confirm

message by pressing any key (except COVER).

x2

Pressing STOP second time will stop centrifuging with the fastest characteristic.

The message can be extinguished with the STOP, SET, COVER,

or

BACK key.

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Summary of Contents for C0226R

Page 1: ... 2018 Corning Incorporated 1 High Performance Refrigerated Centrifuge User Manual C0226R Read it before use ...

Page 2: ...e DANGER Biohazard with potential for risk to health or death as a consequence DANGER Risk of explosion with potential for severe injury or death as a consequence This manual was prepared with special care LABNET INTERNATIONAL may change the manual at any time and without notice Changes will be incorporated in later editions of user manual ...

Page 3: ...is manual is designed to assist you in the optimal usage of your Labnet Refrigerated Laboratory Centrifuge The manual is available in English French German Italian Portuguese and Spanish on our website at www labnetinternational com ...

Page 4: ...CENTRIFUGE OVERVIEW 15 CONSTRUCTION 16 NAME PLATE 16 ROTOR AND ACCESSORIES INSTALLATION 16 CONTROL DEVICE 18 SETTING PARAMETERS 18 SAFETY FEATURES 18 6 CENTRIFUGATION 19 CONTROL PANEL 19 DISPLAY 20 SETTING UP RPM RCF TIME TEMPERATURE 22 USERS PROGRAMS 24 ROTOR CHOOSING 26 SHORT MODE 27 FINISHING THE CENTRIFUGING 28 7 TEMPERATURE CONTROL 29 INITIAL COOLING DURING CENTRIFUGING FAST COOL 29 INITIAL C...

Page 5: ...AGES 38 UNBALANCE 41 9 MENU 42 SCREEN SAVER 43 VISUAL ALARM 43 TYPES OF MAIN SCREEN 43 ROTATING TIME 45 BUZZER 46 DATE TIME 47 LANGUAGE 47 OTHER 47 PASSWORD PROTECTION 48 CYCLES HISTORY 49 TOTAL WORK TIME 49 ROTOR CYCLES 50 DIAGNOSTICS 50 FACTORY SETTINGS 50 MANUFACTURER S DETAILS 51 10 MAINTENANCE 52 CLEANING OF THE CENTRIFUGE 52 MAINTENANCE OF CENTRIFUGE ELEMENTS 52 STERILIZATION 54 CHEMICAL RES...

Page 6: ...he centrifugal force to provide information about their biological Its construction ensures easy operation safe work and wide range of applications at laboratories engaged in routine medical analyses biochemical research works etc This centrifuge is not biotite and therefore during centrifugation of preparations requiring bio tightness one must use bio tightness certificated containers and rotors ...

Page 7: ...temperature with max rotor speed 4 C Cooling without centrifuging yes Acceleration ACEL 10 linear characteristics Deceleration DECEL 10 linear characteristics USB communication yes Electromagnetic compatibility accordance with EN 61326 2 6 2006 Ambient conditions PN EN 61010 1 pkt 1 4 1 Set up site indoor only Ambient temperature 2 40 C Humidity maximum relative humidity 80 Installation category I...

Page 8: ...table lab table Ensure safe location The centrifuge shall not be located near source of heat and shall not be subjected to direct sunlight Centrifuge should be flat levelled Efect of leveling shall be ensure by stable and flat levelled table top for the centrifuge Centrifuge should be set horizontally on a rigid base It is necessary to ensure a ventilation zone of the minimum 30cm round the centri...

Page 9: ...amic loadings Mains socket shall be provided with the safety pin protective earth PE It is recommended to install emergency cut out that shall be located far from the centrifuge near the exit or beyond the room Before switching on check whether the centrifuge is connected to power supply correctly Before using check whether the device is correctly intalled Current protection The centrifuge is equi...

Page 10: ...ry out yearly technical inspections of the centrifuge Manufacturer reserves the right to make technical changes in manufactured products Maximum period of storage of unused centrifuge amounts to 1 year After this period a service authorized by manufacturer should carry out technical inspection of the centrifuge Arrangement of tubes Fix the rotor on the motor axis firmly Avoid unbalance Load opposi...

Page 11: ...m use high vacuum grease HAZARDOUS MATERIALS LABNET accessories are not biotight For centrifuging infectious materials it is necessary to use hermetically closed tubes meeting demands of biotightness in order to prevent germs migration into the centrifuge and beyond it NEVER subject to centrifugation toxic materials with damaged leak proof seals of the rotor or test tube Proper disinfection proced...

Page 12: ...e must use original rotors test tubes and spare parts only In case of faulty operation of the centrifuge one shall ask for assistance service of LABNET INTERNATIONAL Corning or its authorized representatives NEVER switch the centrifuge on if it is not installed properly or rotor is not fitted correctly CENTRIFUGING SUBSTANCES NEVER exceed load limit set by the manufacturer Rotors are intended for ...

Page 13: ...s abrasion of metal parts Screw connections Inspection of bioseals of the buckets if such are used Special attention must be paid to all of the rubber seals parts In the case of damage or visible structural changes defective parts must be replaced for new immediately Control of execution of the guarantee yearly technical inspection of the centrifuge after lapse of guarantee Only the manufacturer s...

Page 14: ...ng in this laboratory centrifuge One should absolutely not use poor quality elements Cracking of glass vessels and test tubes could result in dangerous vibration of the centrifuge NEVER carry out centrifugation with the rotor caps taken off or not driven tight Residual risk The centrifuge is built according to the state of the art and the recognized safety regulations Nevertheless there remains so...

Page 15: ...durable and quiet asynchronous brushless motors and accessories consistent with requirements of the present day user 5 2 Centrifuge overview 1 Motor axle 2 Rotor 3 Rotor lid 4 Complete clamp 1 2 1 Power switch 2 USB 3 Control panel 4 Point of emergency lid opening 5 Lid 6 Inspection glass 1 Plug in socket 2 Fuse socket 4 3 2 1 Fig 1 Right side of centrifuge Fig 3 Assembly of angle rotor Fig 4 Main...

Page 16: ...ories installation Connect the centrifuge to the mains master switch on the back side of the centrifuge Turn on the centrifuge button on the side of the centrifuge Open the lid of the centrifuge by pressing the COVER key see section Centrifuging Control Panel Prior to putting the rotor in one has to check if the rotating chamber is free of impurities e g such as dust glass splinters residues of fl...

Page 17: ...case of centrifuging in an angle rotor test tubes buckets have to be filled properly in order to prevent from pouring fluids during centrifuging Tubes must be filled so that the material does not escape from the reservoir during centrifugation One shall fill tubes according to formula Max liquid level Tube height Internal tube diameter 2 Observe the manufacturer s restrictions about the filling of...

Page 18: ...itched off and the rotor will brake till complete stopping During cover closing it is prohibited to press any buttons Do not place fingers into closing area during cover closing Unbalance detecting When loads of opposite buckets or carriers in rotors are unbalanced the drive will be switched off during acceleration or operation of the centrifuge and the error message will be displayed Rotor verifi...

Page 19: ...gation run STOP2 end centrifugation run COVER cover opening FAST COOL start fast cooling mode BACK RPM RCF exit the current menu cancelling switching between rpm display mode and rcf display mode UP navigation in menu increasing values DOWN navigation in menu decreasing values LEFT navigation in menu RIGHT navigation in menu SET SET changing parameters confirming changes 1 the centrifuge is workin...

Page 20: ...ormal see chapter 9 3 display mode with two sub modes shown below Normal display RPM display mode o RCF display mode Switching between RPM and RCF display mode For normal display switching between RPM and RCF display mode may be obtain by pressing and keeping key by 1s then one should choose demand mode SPEED rotor speed assigned measured RCF centrifugal force assigned measured TIME centrifuging t...

Page 21: ...entrifuging centrifuging with automatic cover opening rotor stopped closed cover rotor stopped opened lid braking fastest decelerating rotor identification thermal chamber temperature delay time delay currently enlarged digits of TIME field drop down list temporarily disabled locked time counting blinking disabled option active option Display Symbols ...

Page 22: ...demanded value Via choose order of magnitude of changing value highlited Repeat above two steps for other orders of magnitude Confirm settings by pressing SET Press BACK When RPM is changed RCF is automatically corrected Exemplary change of RCF setting Press SET to enter edit mode appears Via keys mark RCF field highlighted Press SET blinking With choose demanded value Via choose order of magnitud...

Page 23: ... in 9 3 Main screen modes I Exemplary change of TIME setting Press SET to enter edit mode appears Via keys mark TIME field highlighted 0 0 0 2 00 hh mm ss e g centrifuging time 2 minutes 00 seconds Press SET blinking With choose demanded value Via choose order of magnitude of changing value highlighted Repeat above two steps for other orders of magnitude Confirm settings by pressing SET Exit edit ...

Page 24: ...hlited Press SET key With choose demanded value Confirm settings by pressing SET Press BACK Users programs After switching centrifuge on program that was used in previous session is being loaded If any program was not used in previous session centrifuge resume the last choosen parameters Program choosing Simplified display mode Press and hold by 1 second Choose PROG with Press SET Execute points d...

Page 25: ...rograms is visible Via choose demanded program Confirm with SET key LOAD SAVE DELETE NEW PROGRAM refer choosen program which is highlighted SAVE save settings as a program confirm by selecting YES and pressing SET DELETE delete program confirm by selecting YES and pressing SET NEW PROGRAM enter to create new program mode as below ...

Page 26: ... Centrifugation Changing parameters during centrifuging There is a possibility to change parameters SPEED RCF TIME TEMP during centrifuging Such modifications inactivate currently running program Modification during run is represented by PRG symbol instead of the program number Rotor choosing Rotor choosing Simplified display mode Press and hold by 1 second Choose rotor number exemplary 11199 with...

Page 27: ...nfirm by press SET Press BACK With keys one may switch between screens of rotors parameters It is possible to set AUTOMATIC ROTOR IDENTIFICATION The procedure is described in subsection 9 8 SHORT mode SHORT MODE The SHORT mode is activated by pressing and holding SHORT In SHORT mode the centrifuge is working as long as the SHORT key is pressed or when set time is over ...

Page 28: ...re lapse preselected time one may stop centrifugation Pressing STOP for the first time will stop centrifuging with the charasteristic set in loaded program Confirm message by pressing any key except COVER x2 Pressing STOP second time will stop centrifuging with the fastest characteristic The message can be extinguished with the STOP SET COVER or BACK key ...

Page 29: ...led symbol is visible on the screen blinking Initial cooling during centrifuging FAST COOL The parameters allowable to change at FAST COOL mode temperature lower than current temperature shown by centrifuge In order to centrifuge reduced temperature samples eg storage in the external refrigerator centrifuge chamber rotor and centrifuge container must be pre cooled to the predetermined temperature ...

Page 30: ...y Interruption of the function is signaled by a message Initial cooling without centrifuging THERMAL CHAMBER PARA THERMAL CHAMBER There is a possibility of cooling chamber without centrifuging Way of activate THERMAL CHAMBER is described in chapter Parameters of centrifugation Thermal chamber Cooling in START DELAY OF TEMPERATURE mode PARA START DELAY OF TEMPERATURE Centrifuging with set parameter...

Page 31: ...ing system It allows obtaining selected temperatures in the chamber even at maximum spin speed or fast obtaining desired temperatures e g 4 C Note that time and possibility of obtaining a set temperature is dependent on multiple factors including the power of the cooling system the shape of the rotor the rotor speed ambient temperature etc The accuracy of the temperature stability of 1 C is determ...

Page 32: ...rent screens via keys in PARA field ACCELERATION chosen acc characteristic 0 the fastest 9 the slowest DECELERATION chosen dec characteristic 0 the fastest 9 the slowest RADIUS mm current rotor radius mm DENSITY g cm3 sample density g cm3 TEMP OFFSET O C value of temperature correction CHAMBER DEL min delay between set thermal chamber mode and start it THERMAL CHAMBER cooling of the chamber withou...

Page 33: ... control of the radius of the rotor within the range from Rmin to Rmax Available values depends on chosen rotor see LIST OF ROTORS field Withf keys mark RADIUS Press SET appears Via keys choose demanded values Press SET Press BACK When radius is changed is activated symbol is visible on the screen Displayed RCF will be computed in accordance with changed value of radius Density DENSITY g cm3 defau...

Page 34: ...perature that the cooling system will aim for and set temperature Confirm selection by pressing SET Press BACK Attention The use of the offset can not extend the temperature range achieved by the centrifuge Function description At a set temperature of 20o C and the set offset value equal to 5o C cooling system will actually strive to reach 15o C With a setpoint temperature of 20o C and a set offse...

Page 35: ...ia keys SET demanded temperature Press BACK Activation of thermal chamber is delayed in accordance with information content in chapter 8 5 Thermal chamber delay When THERMAL CHAMBER function is activated symbol is blinking on the screen Changing temperature from the main screen is not possible Opening cover terminates THERM CHAMB function closing cover back turns it on If THERMAL CHAMBER is turned...

Page 36: ... BACK symbol means that OPEN LID AFTER RUN is active Start delay of time Start centrifuging since preselected delay is reached START DELAY OF TIME Via keys mark START DELAY Press SET Via kays mark OF TIME Press SET appears Via keys mark field 0 0 0 05 for example Press SET Start delay can be set from 0 0 0 0 1 to 9 5 9 5 9 Confirm by pressing SET Press BACK When START DELAY OF TIME function is act...

Page 37: ...erature zone Press SET appears Via keys set demanded value Press SET Press BACK When START DELAY OF TEMP is turned on symbol is visible on the screen When the function is active the speed can be reduced to the optimum values for the FAST COOL function when the set speed is lower than the optimum value the rotor rotates at the set speed It is possible to exit START DELAY of temperature mode at any ...

Page 38: ... FINISH OF CENTRIFUGING Additional messages In case of power shortage while centrifuging after repeated switching it on the following error screen will be displayed SUPPLY DECAY WHILE CENTRIFUGING After operating for 2000 hours after every switching on the centrifuge the error screen is being displayed with information about the necessity to carry out servicing activities After pressing the SET ke...

Page 39: ...ed 39 The rotor is automatically updated when auto identification is enabled Rotor is braking only when centrifuge was switched off during rotor running After pressing the SET or STOP key the device returns to the main screen ...

Page 40: ...F ROTATION UNKNOWN ROTOR PLEASE CLOSE THE LID HAND CLOSING THE LID MANUALLY ROTOR STOPPING Please wait INITIALIZING AFTER MAINS FAILURE WITH ROTATING ROTOR CYCLE S ABORTED CENTRIFUGATION ENDED BECAUSE STOP WAS PRESSED CYCLE S FINISHED CENTRIFUGING ENDED WITHOUT ERRORS Emergency messages In case of emergency messages centrifuge is not working properly contact the manufacturer s authorized service c...

Page 41: ...tting the best balance possible Unbalance causes noise and vibrations during operation and adversely affects power transmission system motor shock absorbers The better balance the smoother will be the centrifuge operation and therefore longer life of usage of the driveline Moreover the ideal separation level is then obtained as already separated constituents would not be moved up by vibration 8 12...

Page 42: ... MENU highlighted Press SET Moving in the MENU is possible via keys To open demanded field one should mark it and press SET CONFIGURATION centrifuge configuration PASSWORD password protection LAST 10 CYCLES 10 last centrifugation cycles history WORK TIME total working time working cycles counter ROTOR RUNTIME counting time mode CONTACT US manufacturer s details DIAGNOSTICS error codes service fiel...

Page 43: ... by pressing SET Leave the menu by pressing BACK Visual alarm Visual alarm MENU CONFIGURATION SCREEN MODE Via keys choose VISUAL ALARM Mark it by pressing SET Leave the menu by pressing BACK VISUAL ALARM will cause blinking screen after ending of centrifuging or after error occuring Types of main screen Default setting is NORMAL DISPLAY To switch to SIMPLIFED SCREEN follow the rules in section 9 3...

Page 44: ... keys select SIMPLIFIELD DISPLAY Press SET Leave menu via BACK key Method II Press the BACK button for 1 sec to return to the basic display a short menu is displayed on the screen then Via keys select SIMPLIFIED DISPLAY Press SET 9 3 2 Switching the simplified screen to normal display Press the BACK button for 1 sec to return to the basic display a short menu is displayed on the screen then ...

Page 45: ...ress SET Rotating time The method of counting time centrifuging MENU CONFIGURATION ROTATING RUNTIME Via choose demanded option Mark it by pressing SET Leave menu via BACK key Counting since FROM PRESSING START COUNTING SINCE ROTOR IS IDENTIFIED FROM REACHING SPEED COUNTING FROM ASSIGNED SPEED Presenting mode DESCENDING COUNTING DOWN ASCENDING COUNTING UP ...

Page 46: ...zer Switching ON OFF short audible signals accompanying every pressing of any key MENU CONFIGURATION BUZZER With keys select demanded option Mark selection by pressing SET Leave menu via BACK key Warning signals are always switched on ...

Page 47: ... keys choose demanded menu language Mark it by pressing SET Press BACK Other Rotor automatic identification MENU CONFIGURATION OTHER Thanks to the AUTOMATIC IDENTIFICATION the centrifuge automatically identifies the rotor in the chamber Rotor identification is indicated by the message When the function is deactivated it is necessary to manually select the desired rotor as described in 6 5 Choosing...

Page 48: ...PASSWORD can be set Note No PASSWORD is set by default The PASSWORD can be set as follows when the rotor is at a standstill Press the keys until PASSWORD Press SET appears With keys set the valid 1000s place of thePASSWORD e g 1xxx With keys set correct value Repeat above steps for all places Press SET As a confirmation repeat instructions described above When the PASSWORD is set the Key sign is d...

Page 49: ...ld be used to clear password and remove all locks Cycles history Information concerning parameters of last 10 centrifuging cycles CONFURATION LAST 10 CYCLES Number of cycle can be changed by keys The list can be scrolled using keys To exit press BACK key Total work time Total working time of centrifuge CONFIGURATION WORK TIME In the CYCLES menu the following statistics are displayed total working ...

Page 50: ...list can be scrolled using keys To exit press BACK key Symbols more than 100 cycles left less than 100 cycles left worn rotor Diagnostics Information about errors arisen in working of the centrifuge CONFIGURATION DIAGNOSTICS Use the buttons to select the desired error To exit press BACK key Factory settings Restoring factory setings MENU FACTORY SETTINGS All settings of user programs will be delet...

Page 51: ...bout the type of the centrifuge firmware version and contact details CONFIGURATION CONTACT US CONTACT US LABNET INTERNATIONAL 33 WOOD AVE SOUTH ISELIN NJ 08830 WWW LABNETINTERNATIONAL COM LABNETINFO CORNING COM The listcan be scrolled using keys To exit press BACK key ...

Page 52: ...ck the rotor clamping thread In case of damage replaced it Check the centrifuging chamber whether it is damaged In case of damage it can not be longer put into operation Notify authorized service workshop Maintenance of centrifuge elements In this way the uniform deflection of the buckets and quiet centrifuge operation is ensured Cleaning of the accessories In order to ensure safe operation one sh...

Page 53: ...ctive drying Do not use bleach on plastic parts of the rotor In this way the useful service life of the device is substantially increased and susceptibility to corrosion is diminished Accurate maintenance increases the service life as well and protects against premature rotor failures Do not use bleach on plastic parts of the rotor According to laboratory standards minimize the immersion time in e...

Page 54: ...low density polyethylene EPDM ethylene propylene diene PE HD high density polyethylene NR natural rubber PP polypropylene SI silicon rubber PMP polymethyl pentene One can use all standard disinfectants Centrifuges and devices are made of different materials one should consider their variety radiation radiation 25 kGy C2H4O ethylene oxide formalin ethanol PS SAN PMMA PC PVC POM PE LD PE HD PP PMP E...

Page 55: ...he frequency of autoclaving and use Autoclaving reduce lifespan of plastic and mechanical components PC tubes can become useless Pressure in closed containers can cause plastic deformation or explosion Prior to autoclaving the rotors and accessories thoroughly wash and rinse with distilled water Never exceed the permissible autoclaving temperature and time If you want to keep the hermetic seals re...

Page 56: ...ces cyclic hydrocarbons ahs haloid hydrocarbons alkalis PS SAN PMMA PC PVC POM PE LD PE HD PP PMP ECTFE ETFE PTFE FEP PFA FKM EPDM NR SI DANGER Labnet accessories are not biotite For centrifuging infectious materials it is necessary to use hermetically closed tubes meeting demands of bio tightness in order to prevent germs migration into the centrifuge and beyond it User is responsible for proper ...

Page 57: ...Centrifuge shall be levelled Faulty drive mechanical damage Call service Was centrifuge displaced during operation Switch ON the centrifuge again after opening and closing the cover rotor error After stopping error rotor message is displayed Check if rotor number in started program is consistent with the number of the rotor installed in the centrifuge Check rotor status if there are coding magnets...

Page 58: ...hat rotor is not in the motion use inspection glass Screen failure If the information displayed on the screen disappears and there is no display backlight press the STOP button twice to stop the centrifuging Then make sure to look through the viewfinder into the centrifuge chamber that the rotor has stopped rotating and turn off the power supply with the power switch For reasons of safety do not u...

Page 59: ...ing s Customer Service at the following numbers USA 1 800 492 1110 Canada 1 978 442 2200 For other regions of the world please visit www corning com lifesciences or see the included instruction manual for a list of World Wide Support Offices Corning s Customer Service team will help arrange local service where available or coordinate a return authorization number and shipping instructions Products...

Page 60: ...te Consequently the buyer shall follow the instructions for reuse and recycling of waste electronic and electrical equipment WEEE provided with the products and available at the following link www corning com weee According to Directive 2006 66 EC of 6 September 2006 on batteries and accumulators and waste batteries and accumulators as amended batteries must be collected separately to achieve a hi...

Page 61: ...14 Manufacturer s info CORNING INCORPORATED 800 492 1110 option 2 Customer Service 800 492 1110 Option 5 Service technical support 271 County Route 64 http www labnetinternational com Big Flats NY 14814 e mail labnetinfo corning com ...

Page 62: ... 2018 Corning Incorporated 62 15 Annexes Nomogram ...

Page 63: ...gle rotor for 24 x 1 5 2 0 ml filter tubes spin columns C0226 610 Fixed angle rotor 6 x 10 ml complete with buckets for 17 x 70 85 mm tubes C0226 615 Fixed angle rotor 6 x 15 10ml complete with buckets for 17 x 100 120 mm tubes C0226 125 Fixed angle rotor for 12 x 5 ml tubes C0226 242 Fixed angle rotor 24 x 2 1 5 m tubes C0336 HEM Hematocrit fixed angle rotor ...

Page 64: ... 2018 Corning Incorporated 64 NOTES ...

Page 65: ... 2018 Corning Incorporated 65 ...

Page 66: ... 2018 Corning Incorporated Corning Incorporated 271 County Route 62 Big Flats NY 14814 www labnetinternational com labnetinfo corning com LN182000 ...

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