NanoPhotometer
®
P-Class
User Manual
Version 2.1
Page 65 / 70
10. SPECIFICATION AND WARRANTY
Technical Specifications:
Spectrophotometer
Wavelength range
190 – 1,100 nm
Wavelength scan range
200 – 950 nm
System start up time
Less than 5 seconds, no warm up necessary
Measure time for full scan
range
3.5 seconds
Wavelength reproducibility
< ± 0.2 nm
Wavelength accuracy
± 2 nm
Bandwidth
Better than 5 nm
Stray light
< 0.5% at 220 nm using NaI and 340 nm using NaNO
2
Photometric range
-0.3 – 2.499 A 0-199% T
Detection Range
dsDNA: 0.5 ng/µl to 125 ng/µl, BSA: 0.02 mg/ml to 3.6 mg/ml
Absorbance reproducibility
±0.003 A (0 to 0.5 A), ±0.007 A (0.5-1.0 A) @ 260 nm
Absorbance accuracy
±0.005 A or ±1% of the reading, whichever is the greater
Zero stability
±0.003 A/hour after 20 min warm up @ 340 nm
Noise
0.002 A rms at 0 A @ 260 nm, 0.005 A (pk to pk) at 0 A @ 260 nm
Optical arrangement
Dual channel Czerny Turner with flat grating, 1024 pixel CCD array, concave mirrors
Lamp
Xenon flash lamp
Lifetime
10
9
flashes, up to 10 years
Cell types
15 mm centre height (z-height), outside dimension 12.5 mm x 12.5 mm
NanoVolume application
Detection range
dsDNA: 2 ng/µl to 18,750 ng/µl, BSA: 0.08 mg/ml to 543 mg/ml
Absorbance range
0.01 – 1.5 (10 mm equivalent: 0.05 – 375)
Minimum sample size
0.3 µl
Path lengths
0.04 mm, 0.1 mm, 0.2 mm, 1 mm and 2 mm
Virtual dilution factors
5, 10, 50, 100 and 250
Other technical data
Vortexer
2,800 rpm; tube size up to 2.0 ml
Cuvette storage
capacity for eight 10 mm cells
Photometric mode
Abs, %T, concentration, scan, ratio, multi wavelength, kinetics in Abs x factor / min
Method storage
Up to 81 methods in user methods
Built-in methods
Nucleic acid, microarray (labelling efficiency), protein and cell density
Display formats
320 x 240 pixels
Size
140 mm x 275 mm x 380 mm
Weight
< 4.5 kg
Operating voltage
90-250 V, 50/60 Hz, Max 30 VA
Output ports
Availability of USB flash drive port, USB cable, SD Memory Card or Bluetooth for easy
PC connection and data export on selected models; built-in printer
Input Port
USB cable for firmware updates
Performance verification
Auto diagnostics when switched on
Specifications are measured after the instrument has warmed up at a constant ambient temperature and are typical of
a production unit. As part of our policy of continuous development, we reserve the right to alter specifications without
notice.
Warranty
IMPLEN guarantees that the product supplied has been thoroughly tested to ensure that it meets its published
specification. The warranty included in the conditions of supply is valid for 12 month for the NanoPhotometer
®
P 300 / P 330 and 24 months for the NanoPhotometer
®
P 360 only if the product has been used according to the
instructions supplied. Implen or your supplier can accept no liability for loss or damage, however caused, arising
from the faulty or incorrect use of this product.