NanoPhotometer
®
P-Class
User Manual
Version 2.1
Page 11 / 70
Operation Limitations: Do not autoclave the unit! Do not use an ultrasound bath to clean! Do not drop in water or
solvent bath. The unit is water resistant, but not water proof!
3.2
Software instructions
The
NanoVolume Applications
and
Cuvette Applications
are very similar concerning the analysis of dsDNA, ssDNA, RNA,
Oligonucleotides, protein UV and protein dye analysis. This section describes the specific features which have to be
considered using the NanoPhotometer
®
P-Class Submicroliter Cell. For general information please follow the detailed
instructions under Nanovolume Applications and Cuvette Applications.
The procedure is as follows:
Exemplary Parameter Screen
Parameter Screen
Step 1
Press 1 to select
NanoVolume Applications
folder
Step 2
Press 1 to select
Nucleic Acids
folder OR 2 to select
Protein
folder.
Step 3
Select the method you want to use by pressing the
corresponding number.
Step 4
Select the
Lid Factor
using the left and right arrows.
Lid
Sample volume Pathlength Dilution
5 (optional)
3.5 – 5 µl
2 mm
1:5
10 (optional for P 300)
1 – 3 µl
1 mm
1:10
50
0.3 – 2 µl
0.2 mm
1:50
100 (optional)
0.3 – 2 µl
0.1 mm
1:100
250 (optional)
0.3 – 2 µl
0.04 mm
1:250
Step 5
Select subsequent parameters and specifications as
described under 4.
Nanovolume Applications and
Cuvette Applications.
After the selections are confirmed the results screen displays in
top left corner the chosen Lid and the required sample volume.