NanoPhotometer
®
P-Class
User Manual
Version 2.1
Page 20 / 70
4.2.2
Protein UV Method
The procedure is as follows:
Parameter Screen
NanoVolume Applications
Cuvette Applications
Parameter Screen
Step 1
Press 1 for
NanoVolume
OR 2 for
Cuvette
folder.
Step 2
Press 2 to select
Protein
folder.
Step 3
Press 1 to select
Protein UV
mode.
Step 4
Using NanoVolume Applications select the
Lid Factor
as
described in the “Average Detection Range Sheet” or under
3.2. A minimum of 1.5 µl sample volume (for lid 10) is
recommended.
Step 17
Enter the
Dilution Factor
using the keypad numbers. Range
1.00 to 9,999. Use the C button to backspace and clear the
last digit entered. OR press
Menu/Options
to enter the
dilution factor screen. Enter the volume of the sample
using the keypad numbers. Range 0.01 to 9,999. Enter the
volume of the diluent using the keypad numbers. Range
0.01 to 9,999. Press
OK
to calculate the dilution factor
and return to the Parameters screen OR press
Cancel
to
cancel the selections and return to the Parameters screen.
Step 5
Select whether the
Background
correction at 320 nm is
used or not with the left and right arrows. It
is
recommended
to switch on the
Background
correction.
Step 6
Select the
Protein
(BSA (default), Serum Albumin (mouse),
Serum Albumin (human), IgG, Lysozyme, Custom or OD 1).
Step 7
If using
Custom Protein
there are two possibilities to enter
the correct factors:
Molar extinction coefficient (M
-1
* cm
-1
):
Ranges are:
Wavelength:
200 nm to 340 nm
Molar extinction coefficient
(M
-1
* cm
-1
)
:
10,000 to
9,999,999
Molecular weight:
0.001 to 9,999,999
Extinction coefficient (l/g * cm):
Ranges are:
Wavelength:
200 nm to 340 nm
Extinction coefficient
(l/g * cm)
:
0.001 to 9,999
Step 8
Select the
Units
of measurement using the left and right
arrows. Options: mg/ml, μg/ml, ng/μl and μg/μl.
Step 9
Press
OK
to enter the Results screen OR
Cancel
to
return to the
Protein
folder.