NanoPhotometer
®
P-Class
User Manual
Version 2.1
Page 53 / 70
5.7
Absorbance Ratio
This makes simple absorbance ratio measurements on samples, measuring the amount of light that has passed
through a sample relative to a blank (this can be air) at two wavelengths.
The procedure is as follows:
Parameter Screen
Parameter Screen
Step 1
Press 3 to select
Functions.
Step 2
Press 7 to select
Absorbance Ratio
Step 3
Enter the
first Wavelength
by using the keypad numbers
or the left and right arrows.
Step 4
Enter the
second Wavelength
as above.
Step 5
Select whether a
Background
correction is applied to
both wavelengths 1 and 2 using the left and right arrows.
Step 6
(If background correction is On) Enter the
third
Wavelength
, from which the background correction will
be obtained.
Step 7
Press
Next
to enter the next screen OR press
Cancel
to return to the
Functions
folder.
Step 8
(Dilution Factor known) Enter a
Dilution factor
by using
the keypad numbers within the range 1.00 – 9,999.
Step 9
(Calculate Dilution Factor) Press the
Menu/Options
key.
Enter the
Volume
of the sample (range 0.01 – 9,999),
using the keypad numbers. Enter the volume of
Diluent
(range 0.01-9,999) by using the keypad numbers.
Step 10
Press
OK
to calculate the dilution factor and return to
the Parameters screen OR press
Cancel
to cancel
selections.
Step 11
Select units of measurement, using left and right arrows.
Options are: µg/ml, ng/µl, µg/µl.
Step 12
Enter the factor using the keypad numbers (Range 0.001
to 9,999).
Step 13
Press
OK
to enter the results screen OR
Cancel
to
return to the
Functions
folder.