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colony forming unit (CFU). Since the luminometer is detecting total 

ATP,  it  is  unknown  whether  the  RLU  result  displayed  by  the 

luminometer is due to the detection of microbial ATP, residual ATP, 

or a combination of both. Therefore, a comparison cannot be drawn 

between RLU values  from ATP and standard plate counts (SPC); or 

rather  RLU  does  not  equal  CFU.  True  microbial  contamination  can 

only be determined by a standard plate count method that can take 

up to 48 hours for results. The luminometer is capable of providing 

results  in  15  seconds  offering  a  rapid,  real-time  approach  to 

hygiene/sanitation  monitoring.  The  luminometer  is  not  meant  to 

replace  microbial  testing.  It  can  be  an  effective  tool  when 

incorporated  into  a  Hazard  Analysis  Critical  Control  Point  (HACCP), 

Good Manufacturing Practices (GMP), or sanitation plan. 

1.4

 

Test Devices and Accessories 

Device 

Cat No. 

Description 

UltraSnap 

US2020 

Surface total ATP test device 

SuperSnap 

SUS3000 

High sensitivity total ATP test suited 
for allergen prevention applications 

AquaSnap Total 

AQ100X 

Water total ATP test device 

AquaSnap Free 

AQ100FX 

Water Free ATP test device 

Contamination 
Control Kit 

CCK-4 

Cuvette based water testing kit 

MicroSnap 
Enterobacteriaceae 

MS-EB 

Enterobacteriaceae test 

MicroSnap 
Coliform Test 

MS-COLIFORM  Coliform test 

MicroSnap E. coli 
Test 

MS-ECOLI 

E. coli test 

MicroSnap Total 

MS-TOTAL 

Total Viable Count test 

ZymoSnap ALP 

ZS 

Alkaline Phosphatase test 

CrossCheck 

CX3000 

Acid Phosphatase test 

Calibration Kit 

PCD4000 

Positive and negative rod for 
validating luminometer calibration 

ATP Positive 
Control Kit 

CK25 

25 vials of freeze-dried ATP to verify 
efficacy of ATP test devices 

See  the  Hygiena  website  for  a  complete  list  of  test  devices, 

accessories, and other products. 

www.hygiena.com

 

Summary of Contents for EnSURE v2

Page 1: ...I Ultrasnap SureTrend and dataSURE II are trademarks of Hygiena LLC Operating Precautions and Limitations of Use The following symbol is used in this manual Description CAUTION WARNING IMPORTANT Hygie...

Page 2: ...P PROGRAM TEST POINTS AND PASS FAIL LIMITS _____________________ 10 3 3 TEST PLANS ______________________________________________________ 11 3 4 ESTABLISHING ATP PASS FAIL LIMITS _____________________...

Page 3: ...__ 23 7 TROUBLESHOOTING ________________________________________________ 24 7 1 LUMINOMETER BEEPS _______________________________________________ 24 7 2 TROUBLESHOOTING TIPS __________________________...

Page 4: ...evels of contamination on surfaces water and product samples 1 Remove swab from test tube and collect sample Refer to instructions included with testing device 2 Place swab back in test tube 3 Break S...

Page 5: ...ased from inside these cells along with any free ATP picked up from the surface by the swab is now available to react with the chemistry in the bulb of the device When the Luciferin Luciferase enzymes...

Page 6: ...ng Practices GMP or sanitation plan 1 4 Test Devices and Accessories Device Cat No Description UltraSnap US2020 Surface total ATP test device SuperSnap SUS3000 High sensitivity total ATP test suited f...

Page 7: ...lect and Exit button Up increase button Start accept button Down decrease button Program select button Statistics shortcut button The functions of the keypad are explained in detail in the following s...

Page 8: ...Cell Voltage Relative Capacity Alkaline 1 5V 1 0 NiMH 1 2V 0 6 NiCD 1 2V 0 5 The unit requires two batteries of size AA LR6 or E91 TIP For best results always use a quality brand of Alkaline battery...

Page 9: ...ely The unit will then perform an internal 15 second calibration see section 2 7 NOTE If the clock is not set the unit will automatically enter the time and date set up mode see section 3 8 before per...

Page 10: ...ement reading in Relative Light Units RLU Retested icon identifies the failed test result as having been retested see section 4 2 Retest result icon identifies the Program location or test result as a...

Page 11: ...and is flat TIP The button can be pressed at any time to access the set up menu options see section 3 When the calibration is complete the luminometer is ready to perform a sample measurement For deta...

Page 12: ...button Use the and buttons to scroll through the menu list and then press the button to select the required menu option or the button the exit the menu The following 11 menu options are available User...

Page 13: ...uminometer or set using the SureTrend software If a location name is entered and threshold set in SureTrend and then downloaded to the luminometer the threshold values can t be changed via the luminom...

Page 14: ...ocations specified in the order in which they would normally be tested Having selected the TEST PLAN menu option use the and buttons to scroll through the list of Test Plans and then press the button...

Page 15: ...guide included with your system or visit the resources available on www hygiena com 3 5 Viewing Stored Test Results The luminometer can store a maximum of 2000 test results in its internal memory eve...

Page 16: ...to exit the option 3 8 Setting the Clock Time and Date To set or change the clock time date and format select the CLOCK menu option by pressing the button Scroll to CLOCK and press Then use the and bu...

Page 17: ...ready to perform readings Quick Boot can be turned off and the luminometer will perform a more in depth system check the next time it is turned on The more in depth system check takes 60 seconds NOTE...

Page 18: ...able or disable the warning beeps This will toggle the bell icon from enabled to disabled Press the button to accept the settings increase or decrease duration 5 to 60 set duration enable or disable w...

Page 19: ...corded NOTE The Save Tests mode will be set back to ON when the luminometer is turned off and back on again When Save Tests mode is set to OFF RESULTS NOT SAVED will appear at the bottom of the LCD To...

Page 20: ...y shows the following details Various keypad options are now available as detailed in each of the following sections Button Action Section Access set up menu options 3 Select different User ID 3 1 Sel...

Page 21: ...e displayed WARNING Always ensure that the exterior of the testing device is clean and dry before inserting it into the luminometer Never insert anything other than a Hygiena testing device into the l...

Page 22: ...location was a failure Having selected retest mode signified by the icon next to the Prog perform the sample measurement in the usual way using a new testing device see section 4 1 Once the retest mea...

Page 23: ...on your computer has been disabled Open My Computer right click on the CD drive and select AutoPlay If this does not start the install program open the CD folder and run the Setup application NOTE Yo...

Page 24: ...ardware Wizard does not start check the following a You have sufficient permissions to run the wizard b Verify there are no existing hardware problems in device manager c Verify the Universal Plug and...

Page 25: ...intenance The luminometer does not require any specific routine operator or service engineer maintenance 6 1 Cleaning the Luminometer Case Clean the luminometer case when required using a dry or sligh...

Page 26: ...ocket using water or a very mild detergent solution ensuring that the pocket is completely dry and clean before putting it back into the luminometer WARNING Do not use solvents or other strong chemica...

Page 27: ...use the luminometer emits a variety of different beeping sounds Beep Type Possible Causes Short high pitched tone Luminometer turned on or off Sample measurement started Communications established wit...

Page 28: ...window is dirty Display window is scratched or dented Display or luminometer damaged or faulty Keypad button has no effect when pressed Some buttons only work when selecting particular luminometer fu...

Page 29: ...dirty or severely scratched Luminometer damaged or faulty E4 User settings undefined The user configurable settings are undefined check and re set the CLOCK format User Plan and Prog data E5 Program...

Page 30: ...n of any part of the luminometer or the use or attachment of any peripheral not supplied or specified by Hygiena will void this Warranty WARNING Use only the accessories and consumables supplied by Hy...

Page 31: ...t resolution 1 RLU Measurement time 15 seconds Measurement noise 5 or 5 RLUs User IDs 200 IDs of 16 characters Programmable result thresholds 5001 programs Programmable location names 5001 names of 20...

Page 32: ...alkaline batteries or rechargeable NiMH NiCD batteries of types specified in section 10 WARNING Do not use batteries with individual cell voltages greater than 1 65V as this will cause permanent damag...

Page 33: ...1 The luminometer is manufactured under ISO 9001 controls Declaration of Conformity The luminometer has been designed in accordance with and satisfies the requirements of article 11 of the Low Voltage...

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