APPENDIX - 2
APPENDIX 2. FOR PROPER USE OF
SPECTROPHOTOMETER
2.1 SOLVENT SELECTION
For selecting a solvent in the sample preparation, make sure that
the solvent satisfies following requirements:
Little absorption within the measurement wavelength range.
Not interactive with the solute.
Low volatility.
Table 2-1 shows the applicable wavelength ranges of organic
solvents widely used in common applications. (For details, refer to
“Lectures on Experimental Chemistry” Vol. 15, Part I, edited by the
Chemical Society of Japan.)
Table A2-1 Applicable Wavelength Ranges (Indicated by the solid lines)
Wavelength
Solvent
Cyclohexane
Ethyl alcohol
Methyl alcohol
Ethyl ether
Dioxane
Hexane
Chloroform
Isopropyl alcohol
Acetic acid
Ethyl acetate
Carbon tetrachloride
Benzene
Methyl ethyl ketone
Acetone
Carbon disulfide
200 nm
220 nm
220 nm
220 nm
220 nm
220 nm
250 nm
250 nm
250 nm
270 nm
275 nm
280 nm
335 nm
340 nm
380 nm
200 nm
300 nm
400 nm or more
