10
PIPETMAN
®
u
ltra
8 PIPETTING
Operating procedure
1) Optionally, for Ultra Multichannel set the head to
any position that is comfortable and practicable
for your application. To do this, simply turn the
head to the desired position.
2) Fit new Diamond Tips.
You must fi t tips to your pipette before aspirating and
dispensing any liquid. Plastic tips are for a single ap-
plication – they must not be cleaned for reuse.
3) Pre-rinse the tips.
Some liquids (e.g. protein-containing solutions
and organic solvents) can leave a fi lm of liquid on
the inside the wall of the tip; pre-rinse the tip to
minimize any errors that may be related to this
phenomenon. Pre-rinsing consists of aspirating
the fi rst volume of liquid and then dispensing it
back into the same vessels (or to waste). Subse-
quent volumes that you pipette will have levels
of accuracy and precision within specifi cations.
4) Aspirate.
Press the push-
b u t t o n t o t h e
first stop
( t h i s
c o r r e s p o n d s t o
the set volume of
liquid).
Hold the pipette
vertically (± 20°)
and immerse the
tip in the liquid (see
table for immersion
depth).
Release the push-button slowly and smoothly
(to
top
position) to aspirate the set volume of
liquid.
Wait one second then withdraw the pipette-tip
from the liquid.
Model Immersion
Wait
Time
Depth
(mm)
(seconds)
U2 1
1
U10 1
1
U20 2-3
1
8x20, 12x20
2-3
1
U100 2-4 1
U200 2-4 1
8x300, 12x300
2-3
1
U1000 2-4 2-3
U5000 3-6 4-5
U10 ml
5-7
4-5
Table - Immersion Depth
and Wait Time
Summary of Contents for Pipetman Ultra 12x20
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