7
dispense
Place the end of the tip against the inside wall of the
recipient vessel (at an angle of 10° to 40°).
Press the push-button slowly and smoothly to the
first stop
.
Wait for at least a second, then press the push-button
to the
second stop
to expel any residual liquid from
the tip. Keep the push-button pressed fully down and
(while removing the pipette) draw the tip along the
inside surface of the vessel.
Release the push-button, smoothly. Eject the tip by
pressing firmly on the tip-ejector button.
6 - generaL guIdeLInes For good PIPettIng
Make sure that you operate
the push-button slowly and
smoothly.
When aspirating, keep the
tip at a constant depth
below the surface of the
liquid (refer to the table).
Change the tip before as-
pirating a different liquid,
sample, or reagent.
Change the tip if a droplet remains at the end of the
tip from the previous pipetting operation.
Each new tip should be pre-rinsed with the liquid
to be pipetted.
Liquid should
never
enter the tip-holder; to prevent
this:
- press and release the push-button slowly and
smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there is liquid
in the tip.
If you use the same tip with a higher volume, pre-
rinse the tip.
Table - Immersion Depth
and Wait Time
Model Immersion Wait Time
Depth (mm) (seconds)
P2G
1
1
P10G
1
1
P20G
2-3
1
P100G
2-4
1
P200G
2-4
1
P1000G
2-4
2-3
P5000G
3-6
4-5
P10mlG
5-7
4-5
Summary of Contents for Pipetman P1000G
Page 50: ...NOTES...