P
IP
E
T
TIN
G
9
PIPETMAN® CLASSIC
|
USER’S GUIDE
PIPETTING
|
PIPETMAN® CLASSIC
Dispense
1.
Place the end of the tip against the inside wall of
the recipient vessel at an angle of 10° to 40°.
2. Press the push button slowly and smoothly
to the
first stop
.
3. Wait for at least one second, then press the
push button to the
second stop
to expel
any residual liquid from the tip. Keep the
push button pressed fully down and (while
removing the pipette) draw the tip along the
inside surface of the vessel.
4. Release the push button smoothly. Eject
the tip by pressing firmly on the tip of the
ejector button.
Rest position
First stop
Second stop
Figure 6
Pipetting motion - aspirate and dispense
Guidelines for Good Pipetting
●
Make sure you operate the push button
slowly and smoothly.
●
When aspirating, keep the tip at a constant
depth below the surface of the liquid (refer
to the table).
MODEL
IMMERSION DEPTH
(MILLIMETERS)
WAIT TIME
(SECONDS)
P2
1
1
P10
1
1
P20
2–3
1
P100
2–4
1
P200
2–4
1
P1000
2–4
2–3
P5000
3–6
4–5
P10mL
5–7
4–5
●
Change the tip before aspirating a different
liquid, sample, or reagent.
●
Change the tip if a droplet remains at the end of
the tip from the previous pipetting operation.
Summary of Contents for PIPETMAN Classic P10
Page 1: ...User s Guide EN CLASSIC ...
Page 25: ...Notes 25 PIPETMAN Classic USER S GUIDE NOTES ...
Page 26: ...Notes 26 Notes PIPETMAN Classic NOTES ...
Page 27: ...Notes 27 PIPETMAN Classic USER S GUIDE NOTES ...
Page 28: ...www gilson com contactus LT801120 H 2019 Gilson Inc All rights reserved ...
