Gilson PIPETMAN Classic P10, User Manual

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PIPETTING
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PIPETMAN® CLASSIC
An autoclavable tip ejector extension
can be ordered as an accessory for
P2 and P10 equipped with a plastic tip
ejector (part number F107027; refer to
ACCESSORIES on page 11).
To fit a tip ejector extension:
1. Slide the extension over the tip
holder.
2. Push the extension firmly onto
the end of the tip ejector until it
clicks into place.
To remove a tip ejector extension:
1. Gently twist the extension.
2. Pull it away from the pipette.
Pre-Rinse the Tips
Some liquids (e.g., protein-containing solutions
and organic solvents) can leave a film of liquid
on the inside wall of the tip. Pre-rinse the tip to
minimize any errors that may be related to this
phenomenon.
Pre-rinsing consists of aspirating the first volume
of liquid and then dispensing it back to waste.
Subsequent volumes you pipette will have levels
of accuracy and precision within specifications.
Aspirate
1. Press the push button to the first stop (this
corresponds to the set volume of liquid).
2. Hold the pipette vertically and
immerse the tip in the liquid (refer to
Guidelines for Good Pipetting on page 9).
3. Release the push button slowly and smoothly
(to the top position) to aspirate the set
volume of liquid.
4. Wait one second. The time depends on the
model (refer to the table). Then, withdraw
the pipette tip from the liquid. You may wipe
any droplets away from the outside of the tip
using a medical wipe; however, if you do so,
take care to avoid touching the tip’s orifice.