Gilson PIPETMAN Classic P10 User Manual Download Page 12

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SHO

TROUBLESHOOTING

PIPETMAN® CLASSIC

Chapter 5

TROUBLESHOOTING

A quick inspection of the pipette may help you to

detect a problem.

NOTE

You may download the Two Minute

Inspection Poster from the Gilson

website (

www.gilson.com

), which

shows how to perform a quick

diagnosis of your pipette.

WARNING

Before returning any pipette to

your local Gilson Service Center,

ensure that it is completely free of

chemical, biological, or radioactive

contamination (refer to

Cleaning

and Decontamination

on page 18).

Please use the included safety bag

to return the pipette to your local

Gilson Service Center.

Troubleshooting Table

The following table may help you to identify and

correct issues you might encounter.

SYMPTOM

POSSIBLE CAUSE

ACTION

Pipette is

leaking sample

Damaged tip holder

Replace the tip

holder

Worn O-ring or seal

Replace both parts

Pipette won’t

aspirate

Worn O-ring or seal

Replace both parts

Damaged tip holder

Replace the tip

holder

Connecting nut is

loose

Tighten connecting

nut

Damaged or corroded

piston

Return pipette to

supplier

Improper repair or

assembly

Refer to page 15

Pipette is

inaccurate

Improper repair or

assembly

Refer to page 15

Unscrew tip holder

Tighten connecting

nut

Connecting nut is

loose

Tighten connecting

nut

Summary of Contents for PIPETMAN Classic P10

Page 1: ...User s Guide EN CLASSIC ...

Page 2: ...Tips 8 Guidelines for Good Pipetting 9 ACCESSORIES 11 TROUBLESHOOTING 12 Troubleshooting Table 12 Leak Test 14 MAINTENANCE 15 Changing the Tip Ejector 15 Changing the Tip Holder No Tools Required 17 Servicing the Piston Assembly 17 Cleaning and Decontamination 18 SPARE PARTS 21 P2 F144801 and P10 F144802 22 P20 F123600 and P100 F123615 22 P200 F123601 and P1000 F123602 22 P5000 F123603 and P10mL F...

Page 3: ...acids and bases P5000 and P10mL measurement and transfer of large volumes Parts Checklist After unpacking the pipette verify that the following items were included and are undamaged PIPETMAN Classic PIPETMAN Classic Quick Guide P2 and P10 dual position adapter Safety bag Certiﬁcate of Conformity including barcode sticker GLP Compliant The serial number is engraved on the body of the pipette It pro...

Page 4: ...ted and validated by qualiﬁed technicians in accordance with the Gilson Quality System Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard by type testing The adjustment is carried out under strictly deﬁned and monitored conditions ISO 8655 6 Thumbwheel Body or handle Tip holder PIPETMAN DIAMOND Tip Connecting nut Color coded push button Tip ejector...

Page 5: ...8 0 04 P10 P N F144802 WITH D10 OR DL10 TIPS 1 0 025 0 012 0 12 0 08 5 0 075 0 030 0 12 0 08 10 0 100 0 040 0 12 0 08 P20 P N F123600 WITH D200 TIPS 2 0 10 0 030 0 2 0 1 5 0 10 0 040 0 2 0 1 10 0 10 0 050 0 2 0 1 20 0 20 0 060 0 2 0 1 P100 P N F123615 WITH D200 TIPS 10 0 35 0 10 0 8 0 3 50 0 40 0 12 0 8 0 3 100 0 80 0 15 0 8 0 3 P200 P N F123601 WITH D200 TIPS 20 0 50 0 20 1 6 0 6 100 0 80 0 25 1 ...

Page 6: ... be turned to slowly reach the required setting MODEL COLOR OF VOLUMETER NUMBERS BLACK RED INCREMENT P2 µL 0 01 µL 0 002 µL P10 P20 µL 0 1 µL 0 02 µL P100 P200 µL 0 2 µL P1000 P5000 0 01 mL mL 0 002 mL P10mL mL 0 1 mL 0 02 mL To obtain maximum accuracy when setting the volume When decreasing the volume setting slowly reach the required setting making sure to not pass the setting When increasing th...

Page 7: ...els NOTE For the P2 and P10 models a dual position adapter plastic is required to fit DL10 tips long tips or D10 tips short tips The metallic rod of the tip ejector is shaped so the adapter may be clipped to it in either position P2 and P10 models are delivered with the adapter in place positioned in the longer slot and ready to use DL10 tips When D10 tips are used the adapter must be repositioned...

Page 8: ... to minimize any errors that may be related to this phenomenon Pre rinsing consists of aspirating the first volume of liquid and then dispensing it back to waste Subsequent volumes you pipette will have levels of accuracy and precision within specifications Aspirate 1 Press the push button to the ﬁrst stop this corresponds to the set volume of liquid 2 Hold the pipette vertically and immerse the t...

Page 9: ...e push button smoothly Eject the tip by pressing firmly on the tip of the ejector button Rest position First stop Second stop Figure 6 Pipetting motion aspirate and dispense Guidelines for Good Pipetting Make sure you operate the push button slowly and smoothly When aspirating keep the tip at a constant depth below the surface of the liquid refer to the table MODEL IMMERSION DEPTH MILLIMETERS WAIT...

Page 10: ... temperature of the liquid is different from the ambient temperature pre rinse the tip several times before use You may remove the tip ejector refer to Changing the Tip Ejector on page 15 to aspirate from very narrow tubes After pipetting acids or other corrosive liquids that emit vapors remove the tip holder then rinse the piston O ring and seal with distilled water For the P1000 model use a spec...

Page 11: ...ORIS clips red set of 10 F161302 COLORIS clips yellow set of 10 F161303 COLORIS clips green set of 10 F161304 COLORIS clips blue set of 10 F161305 COLORIS clips white set of 10 F161306 With The JIMMY hands free microtube opener you can open both snap cap and screw cap microtubes to increase pipetting efficiency and reduce the fatigue associated with manual tube opening DESCRIPTION PART NUMBER THE ...

Page 12: ...ncluded safety bag to return the pipette to your local Gilson Service Center Troubleshooting Table The following table may help you to identify and correct issues you might encounter SYMPTOM POSSIBLE CAUSE ACTION Pipette is leaking sample Damaged tip holder Replace the tip holder Worn O ring or seal Replace both parts Pipette won t aspirate Worn O ring or seal Replace both parts Damaged tip holder...

Page 13: ...he tip holder Worn O ring or seal Replace both parts Tips fall off or do not fit correctly Low quality tips Use PIPETMAN DIAMOND Tips Damaged tip holder Replace the tip holder Damaged tip ejector Replace tip ejector Ejector spacer is missing Mount the spacer on the tip ejector The ejector spacer is damaged Replace the ejector spacer The tip ejector is loose Assemble the tip ejector properly The ej...

Page 14: ...pirate the set volume from a beaker of distilled water 4 Maintain the pipette in the vertical position and wait for 20 seconds 5 If a water droplet appears at the end of the tip there is a leak 6 If you do not see a droplet re immerse the tip below the surface of water 7 The water level inside the tip should remain constant If the level goes down there is a leak P1000 to P10mL Models 1 Fit a PIPET...

Page 15: ...ce the push button tip ejector and its dual position adapter With these pipettes if the tip holder is damaged the piston may also be damaged After replacing any parts you should verify the performance of your pipette following the veriﬁcation procedure available on the Gilson website www gilson com If the pipette needs to be readjusted please contact your authorized Gilson Service Center Changing ...

Page 16: ...MAINTENANCE 16 MAINTENANCE PIPETMAN Classic Color coded push button Connecting nut Seal O ring Tip holder Tip ejector Piston assembly Figure 7 Piston assembly disassembled ...

Page 17: ...e Tip Ejector on page 15 Servicing the Piston Assembly You may remove the piston assembly for cleaning purposes only If the piston assembly is changed the pipette must be adjusted and calibrated in a Gilson authorized Service Center NOTICE The piston assembly must not be autoclaved 1 Remove the tip ejector refer to Changing the Tip Ejector on page 15 2 Unscrew the connecting nut by turning it coun...

Page 18: ...eplace the seal followed by the O ring 6 Reassemble the pipette refer to Figure 7 on page 16 7 Tighten the connecting nut turn clockwise 8 Reﬁt the tip ejector refer to Changing the Tip Ejector on page 15 Cleaning and Decontamination PIPETMAN Classic is designed so that the parts normally in contact with liquid contaminants can easily be cleaned and decontaminated However because the models P2 and...

Page 19: ...issue soaked with distilled water 5 Reﬁt the tip ejector and allow the pipette to dry INTERNAL Only the following components can be immersed in a cleaning solution connecting nut tip ejector tip holder piston assembly seal and O ring 1 Disassemble the pipette refer to Figure 7 on page 16 2 Set aside the upper part in a clean dry place 3 Clean the individual components of the lower part of the pipe...

Page 20: ...mination You may choose to decontaminate your pipette chemically in accordance with your own procedures Whatever decontaminant you use check with the supplier of the decontaminant to verify it is compatible with stainless steel and the plastics used in the construction of the pipette PA Polyamide PBT Polybutylene Terephtalate PC Polycarbonate PC PBT Polycarbonate Polybutylene Terephtalate POM Poly...

Page 21: ...PARE PARTS Service Kit 1st level includes 3 piston seals 3 O rings 1 tip holder Service Kit 2nd level includes 1 push button 1 connecting nut 1 tip ejector For only P2 and P10 models 1 tip ejector 1 adapter Only two seals and O ring are provided for P5000mL ...

Page 22: ...TION P20 P100 C D E Service Kit 1st level F144495 F144496 A B F Service Kit 2nd level F107014 F107015 C D Seal Guide O ring 5 sets F144863 F144864 P200 F123601 and P1000 F123602 LABEL DESCRIPTION P200 P1000 C D E Service Kit 1st level F144497 F144498 A B F Service Kit 2nd level F107016 F107017 C D Seal Guide O ring 5 sets F144865 F144866 P5000 F123603 and P10mL F161201 LABEL DESCRIPTION P5000 P10M...

Page 23: ...t Level Service Kit Push button qty 1 for 2nd Level Service Kit Connecting nut qty 1 for 2nd Level Service Kit Tip ejector qty 1 for 2nd Level Service Kit Dual position adapter qty 5 only for P2 and P10 Models Tip ejector qty 1 only for P2 and P10 Models F1 Figure 8 Spare parts identified F Only two seals and O ring are provided for P5000 on the Service Kit 1st level ...

Page 24: ...n repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes Routine cleaning control and recalibration are not covered under the warranty The replacement of wearing parts such as seals o rings broken pistons assembly and broken tip holders are not covered under the warranty NOTICE Yearly routine maintenance is highly recommended to keep yo...

Page 25: ...Notes 25 PIPETMAN Classic USER S GUIDE NOTES ...

Page 26: ...Notes 26 Notes PIPETMAN Classic NOTES ...

Page 27: ...Notes 27 PIPETMAN Classic USER S GUIDE NOTES ...

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