GE Typhoon 9200, Operating Instructions Manual

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4 Operation
4.4 Performing a scanning
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Typhoon Operating Instructions 28-9623-56 AA
a) Click the Orientation button
b) Click the button that represents how the sample is oriented.
c) During the scan, the Typhoon Scanner Control software maps the pixels to
display the image of your sample face up and top-end up.
8 Optional for fluorescent samples: Select Press Sample parameter ( Fig 4-1 , H).
Tick the Press Sample check box if a sample that does not lie flat against the glass
platen of the instrument, such as a dry membrane or filter paper, is to be scanned.
The inner lid of the
Typhoon instrument will go down to press the sample flat.
Note: Do not select Press Sample if you are scanning wet membranes, wet gels, or
soft samples. Pressing wet or soft samples can damage the instrument. In
addition, pressing a soft sample can distort the scanned image.
Note: In the Storage phosphor scan acquisition mode, the Press Sample
parameter will always be used.
9 Select pixel size from the Pixel size list ( Fig 4-1 , I).
Pixel size
(µm) Recommendations
1 000 For a very low resolution scan of the screen.
Produces 10 data points per centimeter and 20 data lines per
grid square.
Note: The 1 000-µm pixel size produces very rough data that is
not recommended for quantitative analysis.
500 For a low resolution scan of the screen.
Produces 20 data points per centimeter and 40 data lines per
grid square.
200 For most standard electrophoresis samples.
Produces 50 data points per centimeter and 100 data lines per
grid square.
100 For samples requiring high resolution (such as DNA sequences).
Produces 100 data points per centimeter and 200 data lines per
grid square.