GE Typhoon 9200, Operating Instructions Manual

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Appendix A Accessories
A.3 Emission filters
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Typhoon Operating Instructions 28-9623-56 AA
A.3 Emission filters
The Typhoon instrument contains a standard set of emission filters and can
accommodate a total of 14 emission filters. The instrument uses emission filters to
reject reflected and scattered excitation light and background fluorescence while
allowing the emitted light from the sample to pass through to the PMT.
Table A-2 lists
the standard set of emission filters used with fluorescence scanning.
Table A-2. Standard set of emission filters.
Emission filter Description
520-nm band-pass filter
(520 BP 40)
(Typhoon 9400 and
9410 only) Transmits light between 500 nm and 540 nm and has a
transmission peak centered at 520 nm.
Use this filter with fluorochromes, such as Cy2 and ECL
Plus™, or with fluorescein when scanning with the 488-
nm wavelength of the blue laser.
555-nm band-pass filter
(555 BP 20) Transmits light between 545 nm and 565 nm and has a
transmission peak centred at 555 nm.
Use this filter with fluorochromes, such as R6G and
HEX.
580-nm band-pass filter
(580 BP 30) Transmits light between 565 nm and 595 nm and has a
transmission peak centred at 580 nm.
Use this filter with fluorochromes, such as TAMRA and
Cy3.
610-nm band-pass filter
(610 BP 30) Transmits light between 595 nm and 625 nm and has a
transmission peak centred at 610 nm.
Use this filter with fluorochromes, such as ROX, EtBr,
SYPRO™ Red, and SYPRO Ruby.
670-nm band-pass filter
(670 BP 30) Transmits light between 655 nm and 685 nm and has a
transmission peak centred at 670 nm.
Use this filter with fluorochromes, such as Cy5.
526-nm short-pass filter Transmits light below 526 nm.
Use this filter with fluorochromes, such as fluorescein
when scanning with the green laser.
Note: When using the blue laser, the 520 BP 40 might
provide better results.
560-nm long-pass filter Transmits light above 560 nm. Use this filter with
fluorochromes, such as TRITC.
390-nm band-pass filter This filter is used for storage phosphor scanning and is
not normally used for fluorescence scanning.