GE AKTAprime plus, Operating Instructions Manual

Page: 39 / 68

1
Connect a sample loop between port 2 and 6 on the injection valve. Make sure that
the sample loop is large enough for your sample.
2 Connect a luer female/1/16" male union to port 3.
3 Fill a syringe with five loop volumes of deionized water or binding buffer.
4
Fit the syringe in the Luer union (1) and carefully inject the buffer.
3
1
5
Remove the syringe and fill it with at least two loop volumes of the sample.
6 Carefully inject the sample into the sample loop. Do NOT remove the syringe after
the injection because the loop might otherwise be emptied due to self-drainage or
air may be introduced in the flow path.
4.4 Performing a run
Selecting template and starting
the run
1
Select Templates in the main menu and press OK.
2
Select Application Template and press OK.
3
Select the appropriate template, for example His Tag Purification HisTrap, and press
OK.
4
Set the sample volume and press OK.
5
Press OK to start the purification run.
ÄKTAprime plus Operating Instructions 28-9597-89 AB 39
4 Operation
4.3 Preparations before start