GE akta start Manual Download Page 7

ÄKTA start System Cue Card, 29-0240-42 AC

7

Perform a manual run

Follow the workflows below to run the system manually.

Operation from the Instrument Display

Manual run

Set 

Run parameters

 to 

suit your requirement

ÄKTA start

End

Run

Exit

Edit run

OK

UNICORN start

System control

Manual run

through 

process picture

Manual 

Instructions

Export a method from UNICORN start

Follow the workflow below to export a method from 
UNICORN start to a USB memory stick. 

UNICORN start

Select 

method to export. 

Select several methods 

using 

Shift 

or 

Ctrl 

keys.

Method editor

Save

File : Export

Export method to USB stick.

Select USB stick location.

Operation from UNICORN start

For detailed instructions on exporting methods, refer to 

the 

UNICORN start 1.0 User Manual

.

Monitoring a run

The user can monitor/control the ongoing run either from 

the ÄKTA start Instrument Display or from UNICORN start. 

Operation from the Instrument Display

The following options are available from 

Run view

 screen: 

View real time values: UV absorbance, Conductivity, 
Flow rate, Pressure, Tube number

Graph

 (view the chromatogram) 

Edit run

 (edit the run parameters of ongoing run)

Hold

 (temporarily holds the run, with set flow rate, valve 

positions, and B concentration sustained)

Pause

 (pauses the run by stopping the pump)

End

 (terminates the current run)




Operation from UNICORN start

System Control

 module is used to start, view, and control 

a run. The instrument is controlled via simple clicks on the 
flow path depicted in the 

process picture

, e.g. to turn the 

valves, set flow rates, change B concentration and start/
stop fractionation.

Import a method to ÄKTA start

Transfer the UNICORN method to USB memory stick, then 
perform the following operations to import a method.

The imported methods are saved in User defined 

methods menu on the ÄKTA start.

USB import

Connect USB stick 

to ÄKTA start

Create method

Select 

method to import

Import

Summary of Contents for akta start

Page 1: ...raphy system intended for preparative purification of proteins at laboratory scale The system can be used for a variety of research purposes to fulfill the needs of the users in the academia and in th...

Page 2: ...w shows the locations of the modules placed on the wet side of the instrument The modules have the following functions To deliver the liquid in the system flow path and divert the flow as required To...

Page 3: ...chromatography runs Allows creation of methods from predefined chromatography templates like Affinity Gel Filtration Ion Exchange and Desalting Gives flexibility to create a customized method by drag...

Page 4: ...n Cond flow cell calibration 1 3 2 System preparation Preparation workflow Instruction Place the buffer bottles A B in the buffer tray and immerse the buffer inlet tubing A B in the respective buffer...

Page 5: ...ple application The sample can be applied to the column in two ways via Pump The sample can be applied directly from a sample container through the Sample valve as shown in the image below It allows a...

Page 6: ...Edit run Method run Prepare system Column preparation Set Run parameters to suit your requirement KTA start Pre defined System preparation Column preparation UNICORN start System control Method run En...

Page 7: ...ument Display or from UNICORN start Operation from the Instrument Display The following options are available from Run view screen View real time values UV absorbance Conductivity Flow rate Pressure T...

Page 8: ...it run in Run view screen when the run is in progress Use appropriate columns as indicated in the template names E g use 1 ml HiTrap column in case of selecting the Quick start AC IEX step 1 ml HiTrap...

Page 9: ...100 B CV NA NA NA NA NA NA 5 0 5 0 NA Re Equilibration Volume CV 5 0 5 0 NA NA 5 0 5 0 5 0 5 0 NA Fractionation Volume ml 1 0 3 0 0 5 3 0 1 0 3 0 1 0 5 0 4 0 the results will be saved only if the USB...

Page 10: ...ration Re equilibration Wash unbound Elution Pump wash Equilibration 100 50 5 CV 5 CV 5 CV 5 CV Sample B Fractionation collection 20 CV G r a d i e n t 0 t o 1 0 0 B GF 16 60 HiPrep Gel Filtration Chr...

Page 11: ...formation visit www gelifesciences com AKTA Selecting protocols columns and buffer Column supported Volume ml Method HisTrap FF 1 or 5 AC HisTrap FF crude 1 or 5 HisTrap HP 1 or 5 GSTrap FF 1 or 5 GST...

Page 12: ...alth Safety Declaration Form for Product Return or Servicing depending on whether the instrument is going to be serviced on site or returned for service respectively Copy the form you need from KTA st...

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