ÄKTA start System Cue Card, 29-0240-42 AC
4
Connect a PC to ÄKTA start
Note: Before connecting the computer to ÄKTA start,
install the UNICORN start software on the computer. For
instructions, refer to the UNICORN start 1.0 User Manual.
Instruction
Connect power to the computer and monitor and start
the computer.
Connect the PC Connection Cable between the
connector marked as PC Connection
at the back of
ÄKTA start and a USB port on the computer.
Launch UNICORN start and connect to ÄKTA start.
Connect Frac30 to ÄKTA start
Note: Frac30 should not be connected or disconnected
when ÄKTA start is switched ON.
Instruction
Connect the Frac30 Cable between the intended ports
at the back of Frac30 and ÄKTA start.
Switch on ÄKTA start.
Enable connection of Frac30 from Instrument Display.
Fractionation
For many purification schemes it is important to collect
fractions of the eluent. ÄKTA start offers the fractionation
options presented in the table below.
1.
2.
3.
1.
2.
3.
Calibration
Calibrate the modules in the order described below
to ensure optimal performance of the system. For
instructions, refer to
ÄKTA start Operating Instructions
.
Instruction
Wash the entire flow path of the instrument with
demineralized (DM) water, then calibrate the modules.
Pump
Flow rate calibration
UV
UV LED calibration
Display
Touch Screen calibration
Pressure Sensor
Zero Offset
Conductivity
Temp. sensor calibration
Cond. flow cell calibration
1
3
2
System preparation
Preparation workflow
Instruction
Place the buffer bottles (A & B) in the buffer tray and
immerse the buffer inlet tubing (A & B) in the respective
buffer bottles (1).
Place the sample inlet tubing in the sample container
or prefill the sample loop with the sample (2).
Place all the waste tubing in the waste bottle (3).
Make sure the pump tubing is installed in the Pump.
Fit the collection tubing to the Fraction collector.
Switch on the instrument.
Calibrate the instrument modules.
1.
2.
3.
4.
5.
6.
7.
A calibration guide for when
to calibrate the modules
is available in
ÄKTA start
Maintenance Cue Card
.
Note: At delivery, the
instruments are pre-
calibrated. Based on the
results of the system
performance test the user
can decide if re-calibrations
are required.
1
3
2
Instrument
configuration
Fractionation options
ÄKTA start +
UNICORN start +
Frac30
Fixed volume fractionation
Peak fractionation
Level based
Slope based
•
•
◊
◊
ÄKTA start +
UNICORN start
Single Peak collection
Level based
•
◊
ÄKTA start + Frac30
Fixed volume fractionation
•
ÄKTA start
Collection of elution volume
•
Prepare the Fraction collector
Instruction
Place a sufficient number of collection tubes (1.5 ml/
5 ml/15 ml) in the Bowl assembly.
Fit the outlet tubing into the tubing holder (1).
Fit the tubing holder into the corresponding port on the
Dispenser arm (2).
Gently move Dispenser arm to dispensing position (3).
Enable Frac30 from Instrument Display (
Home
>
Settings and service
>
Fraction collector
>
Enable Frac
).
Set the delay volume (
Home
>
Settings and service
>
System
>
Delay volume setting
).
1.
2.
3.
4.
5.
6.
