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GE akta start, Manual

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GE akta start Manual Download Page 11

ÄKTA start System Cue Card, 29-0240-42 AC

11

Getting help

ÄKTA start Instrument Display Help

Accessible from every screen on the Instrument Display 
by tapping the 

question mark

 

 located in the upper 

right corner. 
The Display Help text provides information about the 

content of the current screen or refers to more detailed 

documentation.

UNICORN start Online Help

Dialog descriptions of instructions needed to operate 

UNICORN start and analyze chromatography run data.

•

•

•

Flow path details, fraction collection

Note: Fraction collector is enabled.

E.

Flow path details while loading Sample 

through the Pump

F.

Accessible within UNICORN start by pressing 

F1

 or using 

the 

Help

 menu.

The online help covers the system control, method 

editor and result evaluation functions in UNICORN start.

The user documentation available with ÄKTA start is listed 

in section 

Reference information

.

•

•

Reference information

Ordering information

For ordering information visit 
www.gelifesciences.com/AKTA.

Selecting protocols, columns and buffer

Column supported

Volume

(ml)

Method

HisTrap™ FF

1 or 5

AC

HisTrap FF crude

1 or 5

HisTrap HP

1 or 5

GSTrap™ FF

1 or 5

GSTrap HP

1 or 5

GSTrap 4B

1 or 5

HiTrap MabSelect™

1 or 5

HiTrap MabSelect SuRe™

1 or 5

HiTrap rProtein A FF

1 or 5

HiTrap Protein A HP

1 or 5

HiTrap Protein G HP

1 or 5

HiTrap Protein L

1 or 5

HiTrap IgM Purification HP

1

HiTrap IgY Purification HP

5

HiTrap Heparin HP

1 or 5

HiTrap Blue HP

1 or 5

HiTrap Con A 4B

1 or 5

HiTrap TALON® crude

1 or 5

HiTrap IMAC HP

1 or 5

HiTrap IMAC FF

1 or 5

HiTrap Chelating HP

1 or 5

HiTrap Streptavidin HP

1

HiTrap Benzamidine FF

1 or 5

HiTrap NHS-Activated HP

1 or 5

StrepTrap™ HP

1 or 5

MBPTrap™ HP

1 or 5

HiPrep 16/60 Sephacryl S-100 HR

120

GF

HiPrep 16/60 Sephacryl S-200 HR

120

HiPrep 16/60 Sephacryl S-300 HR

120

HiPrep 16/60 Sephacryl S-400 HR

120

HiPrep 16/60 Sephacryl S-500 HR

120

HiTrap Desalting

5

DS

HiPrep 26/10 Desalting

53

HiTrap IEX Selection Kit

1

IEX

HiTrap SP HP

1 or 5

HiTrap Q HP

1 or 5

HiTrap DEAE FF

1 or 5

HiTrap CM FF

1 or 5

HiTrap Q FF

1 or 5

HiTrap SP FF

1 or 5

  

Summary of Contents for akta start

Page 1: ...raphy system intended for preparative purification of proteins at laboratory scale The system can be used for a variety of research purposes to fulfill the needs of the users in the academia and in th...

Page 2: ...w shows the locations of the modules placed on the wet side of the instrument The modules have the following functions To deliver the liquid in the system flow path and divert the flow as required To...

Page 3: ...chromatography runs Allows creation of methods from predefined chromatography templates like Affinity Gel Filtration Ion Exchange and Desalting Gives flexibility to create a customized method by drag...

Page 4: ...n Cond flow cell calibration 1 3 2 System preparation Preparation workflow Instruction Place the buffer bottles A B in the buffer tray and immerse the buffer inlet tubing A B in the respective buffer...

Page 5: ...ple application The sample can be applied to the column in two ways via Pump The sample can be applied directly from a sample container through the Sample valve as shown in the image below It allows a...

Page 6: ...Edit run Method run Prepare system Column preparation Set Run parameters to suit your requirement KTA start Pre defined System preparation Column preparation UNICORN start System control Method run En...

Page 7: ...ument Display or from UNICORN start Operation from the Instrument Display The following options are available from Run view screen View real time values UV absorbance Conductivity Flow rate Pressure T...

Page 8: ...it run in Run view screen when the run is in progress Use appropriate columns as indicated in the template names E g use 1 ml HiTrap column in case of selecting the Quick start AC IEX step 1 ml HiTrap...

Page 9: ...100 B CV NA NA NA NA NA NA 5 0 5 0 NA Re Equilibration Volume CV 5 0 5 0 NA NA 5 0 5 0 5 0 5 0 NA Fractionation Volume ml 1 0 3 0 0 5 3 0 1 0 3 0 1 0 5 0 4 0 the results will be saved only if the USB...

Page 10: ...ration Re equilibration Wash unbound Elution Pump wash Equilibration 100 50 5 CV 5 CV 5 CV 5 CV Sample B Fractionation collection 20 CV G r a d i e n t 0 t o 1 0 0 B GF 16 60 HiPrep Gel Filtration Chr...

Page 11: ...formation visit www gelifesciences com AKTA Selecting protocols columns and buffer Column supported Volume ml Method HisTrap FF 1 or 5 AC HisTrap FF crude 1 or 5 HisTrap HP 1 or 5 GSTrap FF 1 or 5 GST...

Page 12: ...alth Safety Declaration Form for Product Return or Servicing depending on whether the instrument is going to be serviced on site or returned for service respectively Copy the form you need from KTA st...

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