GE akta start Manual Download Page 2

ÄKTA start System Cue Card, 29-0240-42 AC 

2

System overview

Main features of ÄKTA start

ÄKTA start is operated in two modes: 1) using Instrument 
Display (touch screen), and 2) using UNICORN start.
ÄKTA start is offered with a dedicated Fraction collector, 
Frac30.
Quick start methods available for easy purification of 
common tagged/untagged proteins.
Method templates available for Affinity Chromatography 

(AC

), Ion Exchange Chromatography (

IEX

), Gel Filtration 

(GF

) and Desalting (

DS

).

Predefined methods available for cleaning the system 
flow path, flow cells (UV and Conductivity) and testing the 
system performance.

Instrument Display - home screen

Instrument illustration

The illustration below shows the locations of the modules
placed on the wet side of the instrument. The modules 
have the following functions:

To deliver the liquid in the system flow path and divert 
the flow as required
To monitor the UV absorbance and conductivity of the 

liquid

1

2

3

4

5

6

7

8

9

10

Part

Description

1

ÄKTA start

2

Frac30, Fraction collector

3

PC with UNICORN™ start software

Part Description

Part Description

1

Buffer valve

6

Wash valve

2

Mixer

7

Injection valve

3

Sample valve

8

UV Monitor

4

Pump

9

Conductivity Monitor

5

Pressure sensor

10

Outlet valve

1

2

3

The ÄKTA start home screen displays four options for the 

user to select and perform operations from.

Method run

:

 

run predefined methods

Manual run

: start and control the system in real time 

Create method

: create, edit, import and delete methods 

Settings and service

: configure, calibrate, troubleshoot 

and diagnose modules

Summary of Contents for akta start

Page 1: ...raphy system intended for preparative purification of proteins at laboratory scale The system can be used for a variety of research purposes to fulfill the needs of the users in the academia and in th...

Page 2: ...w shows the locations of the modules placed on the wet side of the instrument The modules have the following functions To deliver the liquid in the system flow path and divert the flow as required To...

Page 3: ...chromatography runs Allows creation of methods from predefined chromatography templates like Affinity Gel Filtration Ion Exchange and Desalting Gives flexibility to create a customized method by drag...

Page 4: ...n Cond flow cell calibration 1 3 2 System preparation Preparation workflow Instruction Place the buffer bottles A B in the buffer tray and immerse the buffer inlet tubing A B in the respective buffer...

Page 5: ...ple application The sample can be applied to the column in two ways via Pump The sample can be applied directly from a sample container through the Sample valve as shown in the image below It allows a...

Page 6: ...Edit run Method run Prepare system Column preparation Set Run parameters to suit your requirement KTA start Pre defined System preparation Column preparation UNICORN start System control Method run En...

Page 7: ...ument Display or from UNICORN start Operation from the Instrument Display The following options are available from Run view screen View real time values UV absorbance Conductivity Flow rate Pressure T...

Page 8: ...it run in Run view screen when the run is in progress Use appropriate columns as indicated in the template names E g use 1 ml HiTrap column in case of selecting the Quick start AC IEX step 1 ml HiTrap...

Page 9: ...100 B CV NA NA NA NA NA NA 5 0 5 0 NA Re Equilibration Volume CV 5 0 5 0 NA NA 5 0 5 0 5 0 5 0 NA Fractionation Volume ml 1 0 3 0 0 5 3 0 1 0 3 0 1 0 5 0 4 0 the results will be saved only if the USB...

Page 10: ...ration Re equilibration Wash unbound Elution Pump wash Equilibration 100 50 5 CV 5 CV 5 CV 5 CV Sample B Fractionation collection 20 CV G r a d i e n t 0 t o 1 0 0 B GF 16 60 HiPrep Gel Filtration Chr...

Page 11: ...formation visit www gelifesciences com AKTA Selecting protocols columns and buffer Column supported Volume ml Method HisTrap FF 1 or 5 AC HisTrap FF crude 1 or 5 HisTrap HP 1 or 5 GSTrap FF 1 or 5 GST...

Page 12: ...alth Safety Declaration Form for Product Return or Servicing depending on whether the instrument is going to be serviced on site or returned for service respectively Copy the form you need from KTA st...

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