GE akta start Manual Download Page 3

ÄKTA start System Cue Card, 29-0240-42 AC

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Frac30, Fraction collector

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Part

Description

Part

Description

1

Dispenser arm

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Bowl assembly

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Tubing holder

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Base unit

3

Collection tubes

6

Power indicator

UNICORN start overview

UNICORN start is a software package for:

Controlling the ÄKTA start instrument

Creating and editing methods

Evaluating and analyzing the results from ÄKTA start

Module

Features

System 
Control

Provides an intuitive and easy-to-use 

interface to control ÄKTA start.

Performs and monitors manual 

chromatography runs.

Performs and monitors automated 

predefined or user defined chromatography 
method runs.
Performs system performance method runs.

Method 
Editor

Provides the flexibility to automate the 
chromatography runs.
Allows creation of methods from predefined 
chromatography templates like Affinity, Gel 
Filtration, Ion Exchange and Desalting.
Gives flexibility to create a customized 
method by dragging and dropping the 

chromatography phases such as Prime and 

Equilibration, Sample Application, Elution 
and Fractionation, etc.
Allows exporting methods to a USB flash 
drive for importing them into ÄKTA start.

Evaluation

Allows viewing and presentation of results, 
including creation of PDF reports.
Allows various evaluation operations on 

curves and chromatograms including 

comparison, peak integration etc.
Allows importing results from ÄKTA start and 
exporting results to other formats.

Adminis-
tration

Allows administration of the UNICORN start 
database for backup, restore, archive and 
retrieve operations.
Allows reviewing of UNICORN start and 
system logs.

Safety

Read 

ÄKTA start Operating Instructions

 

before using the instrument.

Summary of Contents for akta start

Page 1: ...raphy system intended for preparative purification of proteins at laboratory scale The system can be used for a variety of research purposes to fulfill the needs of the users in the academia and in th...

Page 2: ...w shows the locations of the modules placed on the wet side of the instrument The modules have the following functions To deliver the liquid in the system flow path and divert the flow as required To...

Page 3: ...chromatography runs Allows creation of methods from predefined chromatography templates like Affinity Gel Filtration Ion Exchange and Desalting Gives flexibility to create a customized method by drag...

Page 4: ...n Cond flow cell calibration 1 3 2 System preparation Preparation workflow Instruction Place the buffer bottles A B in the buffer tray and immerse the buffer inlet tubing A B in the respective buffer...

Page 5: ...ple application The sample can be applied to the column in two ways via Pump The sample can be applied directly from a sample container through the Sample valve as shown in the image below It allows a...

Page 6: ...Edit run Method run Prepare system Column preparation Set Run parameters to suit your requirement KTA start Pre defined System preparation Column preparation UNICORN start System control Method run En...

Page 7: ...ument Display or from UNICORN start Operation from the Instrument Display The following options are available from Run view screen View real time values UV absorbance Conductivity Flow rate Pressure T...

Page 8: ...it run in Run view screen when the run is in progress Use appropriate columns as indicated in the template names E g use 1 ml HiTrap column in case of selecting the Quick start AC IEX step 1 ml HiTrap...

Page 9: ...100 B CV NA NA NA NA NA NA 5 0 5 0 NA Re Equilibration Volume CV 5 0 5 0 NA NA 5 0 5 0 5 0 5 0 NA Fractionation Volume ml 1 0 3 0 0 5 3 0 1 0 3 0 1 0 5 0 4 0 the results will be saved only if the USB...

Page 10: ...ration Re equilibration Wash unbound Elution Pump wash Equilibration 100 50 5 CV 5 CV 5 CV 5 CV Sample B Fractionation collection 20 CV G r a d i e n t 0 t o 1 0 0 B GF 16 60 HiPrep Gel Filtration Chr...

Page 11: ...formation visit www gelifesciences com AKTA Selecting protocols columns and buffer Column supported Volume ml Method HisTrap FF 1 or 5 AC HisTrap FF crude 1 or 5 HisTrap HP 1 or 5 GSTrap FF 1 or 5 GST...

Page 12: ...alth Safety Declaration Form for Product Return or Servicing depending on whether the instrument is going to be serviced on site or returned for service respectively Copy the form you need from KTA st...

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