GE akta start Manual Download Page 1

ÄKTA

 start

System Cue Card

Contents

System overview    2
System preparation    4
Column preparation    6
Starting a run    6
Monitoring a run    7
Quick start methods    8
Flow charts    10
Getting help    11
Reference information    11

System Overview

ÄKTA start is a simple and modern liquid chromatography system intended for 

preparative purification of proteins at laboratory scale. 
The system can be used for a variety of research purposes to fulfill the needs of the 

users in the academia and in the life sciences industry. ÄKTA start is intended for 

research use only, and should not be used in any clinical procedures, or for diagnostic 

purposes.
The System Cue Card describes how to prepare the system for a chromatography run, 

start and monitor a run, and evaluate the result.

Summary of Contents for akta start

Page 1: ...raphy system intended for preparative purification of proteins at laboratory scale The system can be used for a variety of research purposes to fulfill the needs of the users in the academia and in th...

Page 2: ...w shows the locations of the modules placed on the wet side of the instrument The modules have the following functions To deliver the liquid in the system flow path and divert the flow as required To...

Page 3: ...chromatography runs Allows creation of methods from predefined chromatography templates like Affinity Gel Filtration Ion Exchange and Desalting Gives flexibility to create a customized method by drag...

Page 4: ...n Cond flow cell calibration 1 3 2 System preparation Preparation workflow Instruction Place the buffer bottles A B in the buffer tray and immerse the buffer inlet tubing A B in the respective buffer...

Page 5: ...ple application The sample can be applied to the column in two ways via Pump The sample can be applied directly from a sample container through the Sample valve as shown in the image below It allows a...

Page 6: ...Edit run Method run Prepare system Column preparation Set Run parameters to suit your requirement KTA start Pre defined System preparation Column preparation UNICORN start System control Method run En...

Page 7: ...ument Display or from UNICORN start Operation from the Instrument Display The following options are available from Run view screen View real time values UV absorbance Conductivity Flow rate Pressure T...

Page 8: ...it run in Run view screen when the run is in progress Use appropriate columns as indicated in the template names E g use 1 ml HiTrap column in case of selecting the Quick start AC IEX step 1 ml HiTrap...

Page 9: ...100 B CV NA NA NA NA NA NA 5 0 5 0 NA Re Equilibration Volume CV 5 0 5 0 NA NA 5 0 5 0 5 0 5 0 NA Fractionation Volume ml 1 0 3 0 0 5 3 0 1 0 3 0 1 0 5 0 4 0 the results will be saved only if the USB...

Page 10: ...ration Re equilibration Wash unbound Elution Pump wash Equilibration 100 50 5 CV 5 CV 5 CV 5 CV Sample B Fractionation collection 20 CV G r a d i e n t 0 t o 1 0 0 B GF 16 60 HiPrep Gel Filtration Chr...

Page 11: ...formation visit www gelifesciences com AKTA Selecting protocols columns and buffer Column supported Volume ml Method HisTrap FF 1 or 5 AC HisTrap FF crude 1 or 5 HisTrap HP 1 or 5 GSTrap FF 1 or 5 GST...

Page 12: ...alth Safety Declaration Form for Product Return or Servicing depending on whether the instrument is going to be serviced on site or returned for service respectively Copy the form you need from KTA st...

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