GE akta start, Manual

Page: 12 / 12

29-0240-42 AC 07/2014
For local office contact information, visit
www.gelifesciences.com/contact
GE Healthcare Bio-Sciences AB
Björkgatan 30, SE-751 84 Uppsala, Sweden
www.gelifesciences.com/AKTA
GE and GE monogram are trademarks of General Electric Company.
ÄKTA, GSTrap, HiPrep, HiTrap, HisTrap, MabSelect, MBPTrap,
StrepTrap, Superloop, SuRe, and UNICORN are trademarks of
General Electric Company or one of its subsidiaries.
TALON is a registered trademark of Clontech Laboratories Inc.
All other third party trademarks are property of their respective
owners.
Any use of UNICORN is subject to GE Healthcare Standard Software
End-User License Agreement for Life Sciences Software Products.
A copy of this Standard Software End-User License Agreement is
available on request.
UNICORN start © 2013-2014 General Electric Company
© 2013-2014 General Electric Company – All rights reserved.
First published Sep. 2013
All goods and services are sold subject to the terms and conditions
of sale of the company within GE Healthcare which supplies them. A
copy of these terms and conditions is available on request. Contact
your local GE Healthcare representative for the most current
information.
GE Healthcare Europe GmbH
Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare UK Limited
Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327,
USA
GE Healthcare Japan Corporation
Sanken Bldg. 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073,
Japan
Health and Safety Declaration Form
Please complete the checklist in the On Site Service
Health & Safety Declaration Form or the Health & Safety
Declaration Form for Product Return or Servicing,
depending on whether the instrument is going to be
serviced on site or returned for service, respectively.
Copy the form you need from ÄKTA start Operating
Instructions , Section 10.4 Health and Safety Declaration
Form , or print it from the PDF file available on the User
Documentation CD.
User documentation for ÄKTA start
Documentation Book PDF Web Online
Operating Instructions - • • -
Maintenance Manual - • • -
System Cue Card • • • -
Maintenance Cue Card • • • -
UNICORN start 1.0 User
Manual
- • • -
UNICORN start Online
Help - - - •
Display Help - - - •
Buffer selection
Buffer types needed
For AC: Binding buffer
Elution buffer
Wash buffer (optional) •
•
•
For DS: One buffer type per run •
For IEX: Binding buffer
Elution buffer
Wash buffer (optional) •
•
•
For GF: One buffer type per run •
AC buffer suggestions for His-tagged proteins
If performing... suggested buffer
binding using HisTrap
or HiTrap Chelating 50 mM Tris-HCl pH 7.5, 0.5 M
NaCl, 20-40 mM imidazole
1
wash 50 mM Tris-HCl pH 7.5, 0.5 M
NaCl, 100 mM imidazole
step elution 50 mM Tris-HCl pH 7.5, 0.5 M
NaCl, 500 mM imidazole
1
The imidazole concentration is protein dependent.
AC buffer suggestions for GST-tagged proteins
If performing... suggested buffer
binding using
GSTrap 4B, HP or FF 50 mM Tris-HCl pH 7.5, 150 mM
NaCl, 1 mM DTT
step elution 50 mM Tris-HCl, 10 mM
reduced gluthathione, pH 8
DS buffer suggestions
If performing... suggested buffer
Buffer exchange/
desalting
50 mM Tris-HCl pH 8.0
IEX buffer suggestions
If performing... suggested buffer (depends on
the pI of the protein)
binding to AIEX 50 mM Tris-HCl pH 8.0
binding to CIEX 20 mM MES pH 6.0
elution from AIEX 50 mM Tris-HCl, pH 8.0, 1 M
NaCl
elution from CIEX 20 mM MES pH 6.0, 1 M NaCl
GF buffer suggestions
If performing... suggested buffer
Buffer exchange/
size exclusion
chromatography
a suitable buffer, e.g. 50 mM
Tris-HCl pH 7.5, 150 mM NaCl