MAW
®
User Manual Rev. 3.0
20
Figure 12.11
Gas in compartments
Overview of the gas system
Required input gas type: premixed CO
2
. Check medium types for the correct mixture and
validate the mixture with a gas analyzer prior to use.
Required input gas pressure: gas pressure on the external source should be 0.6 bar (8.70
PSI) and it must be kept stable.
Gas alarm: the alarm goes off when the gas level drifts <0.2 bar and >0.8 bar. If an alarm goes
off, remove the sample to the safe CO
2
incubator and investigate the cause of the alarm.
The gas flow setpoint can be adjusted in the range from 0 l/h to 40 l/h. 1 l/h increments.
The correct flow level is a balance that helps to maintain the right pH level in the medium
and ensures the economy of gas usage. An increased flow increases better chances to main-
tain correct gas level and fast gas recovery, however, it also increases the cost of gas. Thus,
the flow may be adjusted down to the level where the pH in the medium is still maintained
and the gas consumption is as low as possible. This aspect in the specific medium may be
determined only by a local validation test. Please a
lso check the medium manufacturer’s
recommendations.