Dionex UltiMate 3000 Series, Operating Instructions Manual

Page: 63 / 236

UltiMate 3000 Series:
SD, RS, and BM Pumps
Operating Instructions Page 49

Make sure that the tubing connecting the pump to the degasser is as short as possible and
locate the solvent reservoirs as close as possible to the pump. To avoid formation of gas
bubbles in the reservoirs and reformation of gas bubbles in the solvent, make sure that the
reservoirs are on the same level or higher as the pump. Therefore, stack the rack onto the
pump as shown in Fig. 12.

Before connecting the solvent lines, make sure that the connectors are free of
contaminants. Even minute particles can allow air to enter the degasser, and thus reduce
the degassing effectiveness.

Normal phase eluents usually show only a low concentration of dissolved gases.
Therefore, it is normally not required to use a degasser with these eluents.
4.3.2 Connecting the Solvent Reservoirs
4.3.2.1 All pumps except ISO-3100BM
1. Feed the solvent line through the retaining guide, which holds the tubing in place in
the reservoir, and then into the open hole in the reservoir cap.
2. Slide the filter holder with filter frit onto the end of the solvent line.
3. If required
Verify that the end of the solvent line is cut straight and is not deformed. Cut the tubing
straight if necessary. Use only the original Dionex solvent lines.
4. Place the entire assembly in the solvent reservoir.
5. Tighten the reservoir cap hand-tight. Press the retaining guide into the hole in the
reservoir cap to hold the solvent line in place inside the reservoir.
Fig. 13: Connecting the solvent lines to the reservoirs
When replacing a solvent line, remove the frit first, then the retaining guide, and then the
solvent line.
Retaining guide
Filter holder with
filter frit