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UltiMate 3000 Series:

SD, RS, and BM Pumps

Operating Instructions

Page

45

4 Preparation for Operation (Startup)

4.1 Overview of Actions

Important:

The pump is filled with 2-propanol when being shipped from the
factory. During initial operation of the module, make sure that the
solvents used are miscible with 2-propanol. Otherwise, follow the
appropriate intermediate steps.

Important:

La pompe est stockée sous 2-propanol lorsqu'elle est expédiée depuis
l'usine. Lors du démarrage initial de la pompe, assurez-vous que les
solvants utilisés sont miscibles. Dans le cas contraire, utilisez un
solvant intermédiaire approprié. Même d'infimes particules peuvent
endommager le système.

After you have unpacked, positioned and connected the pump as described in sections 3.1
through 3.4 (



page 27 and following pages), prepare the pump for operation. Follow this

sequence of steps:

1.

If the UltiMate 3000 system includes an SRD-3x00 Solvent Rack

Connect the 15-pin D-Sub port on the rear of the Solvent Rack with the Solvent Rack port
on the rear of the pump (



2.

If you want to connect an ESA Corona or Coulochem III detector to the pump

Refer to section 3.6 for details (



Before

you attach the column and cell, flush the system with several volumes of the

mobile phase to be used.

3.

Connect the pump to the other modules of your UltiMate 3000 system, as required by
your application.
When you connect capillaries to the pump, observe the general precautions on page 47.

4.

Connect the solvent reservoirs, and then connect the solvent lines to the degassing
channels (



5.

Set up the rear seal wash system (



page 50) and flush the system with the seal-

washing liquid (



page 51).

6.

Connect drain tubing (



This is a

must

as also the seal washing liquid is directed to waste through the drain

system. If drain tubing is not connected, modules that are located below the pump in the
UltiMate 3000 system stack, may suffer severe damage from the liquid leaving the drain
port.

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Summary of Contents for UltiMate 3000 Series

Page 1: ...UltiMate 3000 Series SD RS and BM Pumps Operating Instructions Original Operating Instructions Revision 1 4 Date September 2010 2010 Dionex Doc No 4820 4001 ...

Page 2: ...UltiMate 3000 Series SD RS and BM Pumps Operating Instructions ...

Page 3: ...chinery risk assessment part 1 The electrical safety of the products was evaluated based on the following standard EN 61010 1 2002 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General Requirements The Electromagnetic Compatibility EMC of the products was evaluated based on the following standard DIN EN 61326 2006 Electrical equipment for measuremen...

Page 4: ...UltiMate 3000 Series SD RS and BM Pumps Page II Operating Instructions ...

Page 5: ...UltiMate 3000 Series SD RS and BM Pumps Operating Instructions Page III ...

Page 6: ...UltiMate 3000 Series SD RS and BM Pumps Page IV Operating Instructions ...

Page 7: ...UltiMate 3000 Series SD RS and BM Pumps Operating Instructions Page V ...

Page 8: ...UltiMate 3000 Series SD RS and BM Pumps Page VI Operating Instructions ...

Page 9: ...Special Information for Biocompatible Pumps 14 2 3 Operating Principle 15 2 4 Front Panel Elements 16 2 5 Rear Panel 17 2 5 1 Power Switch 18 2 5 2 Fuse Cartridge 18 2 5 3 USB Connector 18 2 5 4 Digital I O 19 2 5 5 Solvent Rack 19 2 6 Fluid Connections 20 2 7 Rear Seal Wash System 21 2 8 Mixing System and Inline Filter 22 2 9 Purge Unit 23 2 10 Leak Sensor 23 2 11 Vacuum Degasser 24 2 12 Pulse Da...

Page 10: ... All pumps except ISO 3100BM 49 4 3 2 2 ISO 3100BM 50 4 4 Setting Up the Rear Seal Wash System 51 4 5 Connecting Drain Tubing 53 4 6 Purging the Pump 54 4 6 1 Purging the Pump Manually 55 4 6 2 Purging the Pump By Using the Autosampler 57 4 7 Equilibrating the System 58 5 Operation and Maintenance 61 5 1 Power Up 61 5 2 Status Screens 62 5 3 Operation with Chromeleon 64 5 3 1 Connecting to Chromel...

Page 11: ... 91 5 6 2 Pump Diagnostics 93 5 6 3 Setting a Gradient Curve 94 5 6 4 Liquid Level Monitoring for Solvent Reservoirs and Waste Container 94 5 6 5 Using the Digital Inputs and Outputs Digital I O 96 5 6 6 Operational Qualification and Performance Qualification 96 5 7 Shutting Down the Pump 97 5 8 Routine and Preventive Maintenance 99 6 Troubleshooting 103 6 1 Overview 103 6 2 Pump Block Status Indi...

Page 12: ... Filter ISO 3100 149 7 7 2 Inline Filter LPG 3400BM DGP 3600BM 150 7 7 2 1 Replacing the Inline Filter 150 7 7 2 2 Replacing the Filter Frit in the Inline Filter 151 7 7 3 Checking the Inline Filter for Permeability 152 7 8 Replacing the Purge Valve Knob 153 7 9 Testing the Pump for Leakage 154 7 10 Vacuum Degasser Rinsing the Degassing Channels 157 7 11 Replacing the Main Power Fuses 158 7 12 Upd...

Page 13: ... 181 9 Optimizing the Pump for Specific Applications 183 10 Technical Information 187 11 Accessories Consumables and Spare Parts 191 11 1 Standard Accessories 191 11 2 Optional Accessories 197 11 3 Consumables and Spare Parts 199 12 Reference Information 207 12 1 Chemical Resistance of PEEK 207 12 2 Solvent Miscibility 210 12 3 Properties of Common Solvents 211 12 4 Safety Information about Flamma...

Page 14: ...UltiMate 3000 Series SD RS and BM Pumps Page vi Operating Instructions ...

Page 15: ...necessarily apply to your particular pump The optical design of some components may differ from the representation in the pictures in this manual but this does not affect the descriptions The descriptions in this manual refer to firmware version 3 00 and Chromeleon 6 80 Service Release 10 If you want to operate the pump with Chromeleon 7 note the information on page 25 The information contained in...

Page 16: ...alternatif Power supply is on L instrument est mis sous tension and Power supply is off O L instrument est mis hors tension O Refer to the Operating Instructions to prevent risk of harm to the operator and to protect the instrument against damage Référez vous à ce manuel pour éviter tout risque de blessure à l opérateur et ou protéger l instrument contre tout dommage Label according to the Measure...

Page 17: ...ial hazards of using chemical solvents Wear appropriate protective clothing Tips Before initial operation of the pump make sure that you are familiar with the contents of this manual Observe any warning labels on the pump and see the related sections in these Operating Instructions To avoid the possibility of personal injury and damage to the instrument observe the following general safety precaut...

Page 18: ... seals When connecting the capillaries make sure that the connectors are free from contaminants Even minute particles may cause damage to the system for example to the column After operation rinse out buffers and solutions that form peroxides Before switching from buffer to organic solution rinse the pump thoroughly with deionized water When switching to another solvent ensure that the new solvent...

Page 19: ... accumulate Follow a regulated approved waste disposal program Never dispose of flammable or toxic solvents through the municipal sewage system Use only standard solvents HPLC grade and buffers that are compatible with all parts that may be exposed to solvents In an UltiMate 3000 system some components are made of PEEK While this polymer has superb chemical resistance to most organic solvents it t...

Page 20: ...vant bascule vers le haut Afin d éviter d endommager la pompe lorsque que vous la soulevez ou la déplacez saisissez la toujours par les côtés de l unité Ne placez aucun objet lourd sur la porte ouverte du panneau avant Ceci pourrait endommager la porte Réglez toujours une limite de pression minimum pour la pompe HPLC Ceci prévient les dommages résultant de fuites ou de long terme fonctionnement à ...

Page 21: ...e sans rinçage du joint arrière pendant un temps prolongé 5 minutes Ceci peut endommager les joints de piston et le piston Remplacer régulièrement le liquide dans le réservoir du système de rinçage du joint arrière au moins une fois par semaine Utilisez toujours les frittés recommandés par Dionex afin d empêcher les particules étrangères d entrer dans le système HPLC Utiliser d autres frittés peut...

Page 22: ...sées Utilisez uniquement des solvants qualité HPLC et des solutions salines compatibles avec les matériaux exposés phase mobiles Dans un système UltiMate 3000 certaines composantes sont en PEEK Bien que ce polymère présente une excellente résistance chimique à la plupart des solvants organiques il a tendance à gonfler lorsqu il est en contact prolongé avec du chloroforme CHCl3 du diméthyle sulfoxy...

Page 23: ...that the pump may be operated only with Dionex accessories and spare parts page 191 and within its technical specifications page 187 If there is any question regarding appropriate usage contact Dionex before proceeding Dionex cannot be held liable for any damage material or otherwise resulting from inappropriate or improper use of the pump 1 4 Federal Communications Commission FCC Note This equipm...

Page 24: ...UltiMate 3000 Series SD RS and BM Pumps Page 10 Operating Instructions ...

Page 25: ...ng the robustness of the pump As a standard all pumps are equipped with an active rear seal wash system page 21 Various monitoring and diagnostic features are provided for optimum system performance and reliability page 26 The pump is designed for easy access to the fluid components allowing fast and reliable maintenance while the instrument remains in the UltiMate 3000 system stack For the secure...

Page 26: ...as LPG 3400SD however as biocompatible micro pump working pressure up to 50 MPa 7250 psi 5042 0036 DGP 3600SD Dual low pressure gradient pump analytical Two separate pumps in one enclosure 2 x 3 solvents working pressure up to 62 MPa 9000 psi 5040 0061 DGP 3600RS Same as DGP 3600SD however working pressure up to 103 MPa 15000 psi 5040 0066 DGP 3600BM Same as DGP 3600SD however as biocompatible mic...

Page 27: ...lly for use with the following pumps one HPG 3200 one ISO 3100SD 5035 9250 SRD 3400 Solvent Rack with analytical 4 channel vacuum degasser typically for use with the following pumps one HPG 3400 two HPG 3200s in a two stack system one HPG 3200 or ISO 3100SD if you want to degas the solvents and the wash liquid of an UltiMate 3000 series autosampler 5035 9245 SRD 3600 Solvent Rack with analytical 6...

Page 28: ...nium parts are lighter than parts made of stainless steel Nevertheless you can easily confuse titanium with stainless steel parts Observe the following When substituting parts be sure to install the appropriate replacement part for the BM pumps For information about the part numbers and for installation details see the related service sections page 123 and following pages and the Consumables and S...

Page 29: ...inder carries out the suction stroke The characteristic feature of the patented isokinetic pre compression is the overlapping phase of the delivery strokes of the working and equilibration cylinders When delivering compressible liquids without controlled pre compression the pulsation increases as the working pressure increases since part of the delivery stroke is required for compressing the solve...

Page 30: ... is red To cancel Standby mode and resume operation press the Standby button again the LED is not lighted Notes To allow the pump to change the mode press and hold the Standby button for at least 1 second If you switch a pump to which an SRD 3x00 Solvent Rack is connected to the Standby mode the Solvent Rack too will be set to Standby mode 3 LEDs Power The LED is blue when the pump is turned on Co...

Page 31: ... O ports for communication with external devices page 19 5 Solvent Rack port for connection of an SRD 3x00 Solvent Rack page 19 6 USB hub 3 USB ports USB 2 0 or 1 1 Depending on the UltiMate 3000 system configuration for connection of one UltiMate 3000 system module each or for connection of one USB hub each page 18 7 USB port USB 2 0 or 1 1 for connecting the pump to the Chromeleon computer page ...

Page 32: ...ow to change the fuses see page 158 2 5 3 USB Connector The Chromeleon Chromatography Management System can use a USB connection to control the pump Data is transferred digitally by the appropriate USB cable page 30 To ensure trouble free operation use only the cables shipped with the pump You can use the internal USB hub Fig 2 no 10 to connect three other instruments in the UltiMate 3000 product ...

Page 33: ...e 215 Tip If you want to connect an ESA Corona or Coulochem III detector to the pump refer to page 43 for details 2 5 5 Solvent Rack Use this port to connect an SRD 3x00 Solvent Rack with integrated vacuum degasser to the pump Important Do not substitute any other Solvent Rack for the Solvent Racks mentioned in the table on page 13 Important Ne remplacez les dégazeurs de la série SRD 3x00 mentionn...

Page 34: ...mmager la porte Lorsque vous soulevez ou déplacez la pompe saisissez la toujours par les côtés de l instrument Soulever la pompe par le panneau avant risque d endommager la porte du panneau avant Capillary guides on the pump bottom facilitate routing the capillaries to devices that are located below the pump in the UltiMate 3000 system stack Fig 3 Capillary guides on the pump bottom See section 8 ...

Page 35: ... The seal washing liquid passes the individual components as shown in Fig 4 Fig 4 Rear seal wash system here for a pump with two pump heads The arrows indicate the flow path of the wash liquid through the pump No Description No Description 1 Liquid reservoir of the rear seal wash system 4 5 Pump head 2 Peristaltic pump page 50 6 Detector 3 Peristaltic tubing Pharmed page 50 7 To waste For informat...

Page 36: ... µL HPG 3x00RS Mixing volume 200 µL including Capillary mixer volume 50µL Static mixer volume 150 µL In the capillary mixer the solvent streams delivered by the pump are combined and premixed before they enter the static mixer The static mixer improves the mixing quality of the combined solvent streams and thus guarantees a smoother baseline Fig 5 Two step mixing system here in an HPG 3400SD In ad...

Page 37: ...nsor A leak sensor is installed inside the pump If liquid collects in the drip tray under the fluid connections the leak sensor reports a leak The Status LED on the front panel door changes to red a beep sounds and a message appears on the pump display and in the Chromeleon Audit Trail When the leak sensor reports a leak eliminate the cause for the leakage and dry the leak sensor page 124 Leak det...

Page 38: ...ash liquid on a continuous basis The procedure how to prepare and install the wash liquid lines is similar to the steps for the solvent supply page 49 For more information see the Autosampler Manual 2 12 Pulse Damper ISO 3100BM pumps have an integrated pulse damper that reduces the already low pulsation of the pump even further Technical Information page 187 The pulse damper consists of a reservoi...

Page 39: ...about automatic control see page 68 Tip All software details in this manual refer to Chromeleon 6 80 If you want to operate the pump with Chromeleon 7 refer to the following documents for information about how to perform the related processes in Chromeleon 7 all documents are included in the Chromeleon 7 shipment Chromeleon 7 Help provides extensive information and comprehensive reference material...

Page 40: ... power up Leak sensor page 23 Active rear seal wash system page 21 When an error is detected the Status LED on the front panel door is red a beep sounds and a message appears on the pump display page 105 When the pump is operated with Chromeleon additional functions for estimating the lifetime of consumables and monitoring and recording service and re qualification information predictive performan...

Page 41: ...f mechanical damage which might have occurred during transit Tips Immediately report any shipping damage to both the incoming carrier and Dionex Shipping insurance will compensate for the damage only if reported immediately Keep the original shipping container and packing material They provide excellent protection for the pump in case of future transit Shipping the pump in any other packaging auto...

Page 42: ...ate After 4 hours check the pump if condensation still exists allow the pump to continue to warm up without connecting it to the power source until the condensation is completely gone 3 3 Positioning the Pump in the UltiMate 3000 System If the pump is part of an UltiMate 3000 system for example for analytical HPLC applications you should stack the individual modules for example as shown in Fig 7 a...

Page 43: ...he USB port on the rear panel of the instrument However Dionex recommends interconnecting all modules and then connecting the system to the Chromeleon computer with only one connection For systems with a DAD 3000 RS or MWD 3000 RS you can use only the hub on the detector for the connection For systems with a VWD 3x00 RS use only the hub on the pump Autosampler Column Compartment Pump Solvent Rack ...

Page 44: ...rear panel Fig 2 page 17 Select one of the following alternatives Connect the pump directly to the USB port on the computer Connect the pump to an internal USB port on another module in the UltiMate 3000 system that is connected to the computer page 29 Connect the pump to the computer by using an external USB hub if necessary However this may be the source for communication problems depending on t...

Page 45: ... the rear panel of the Solvent Rack The appropriate connection cable is included in the accessories pack of the Solvent Rack Digital I O To connect devices to the digital I O ports on the rear panel of the pump use a 6 pin mini DIN signal cable The cable is available from Dionex part no 6000 1004 1 Plug the 6 pin connector of the mini DIN cable into the Digital I O port 1 or 2 For information abou...

Page 46: ...uter 3 Open the Chromeleon Server Monitor program by double clicking the Chromeleon Server Monitor icon on the Windows taskbar If the Server Monitor icon is not on the taskbar click Start on the taskbar point to Programs or All Programs depending on the operating system point to Chromeleon and then click Server Monitor 4 Click Start to start the server 5 Click Close to close the Server Monitor win...

Page 47: ...t to Windows Update to search for software select No not this time b Accept the default option Install the software automatically and click Next c Click Finish when the wizard reports that the software for the pump has been installed If Windows fails to detect the pump and a message box asks for a USB configuration file cmwdmusb inf this indicates that you connected the pump to the computer and tu...

Page 48: ...name to display the items underneath 4 Select the timebase to which the pump will be assigned or create a new timebase on the Edit menu click Add Timebase 5 Open the Add device to timebase dialog box To do so click Add Device on the Edit menu or right click the timebase and click Add Device on the menu 6 On the Manufacturers list click Dionex HPLC UltiMate 3000 and on the Devices list click the pu...

Page 49: ...settings for a specific application in the Commands dialog box in a program file PGM or on a control panel will not change the default settings on the configuration pages For additional information about a page click Help General Page Display general information about the pump Fig 9 General page Device Type Indicates the pump model Enable Virtual Mode This check box should be cleared because Chrom...

Page 50: ...e button appears dimmed if the virtual mode is enabled The pump is shipped with the most recent firmware version If a firmware update is ever required follow the steps in section 7 12 page 159 Devices Page The pump type determines the settings and options on this page Fig 10 Devices page here for a DGP 3600RS Main Device Name default name PumpModule Displays the name used to identify the pump in t...

Page 51: ... if the pump is an LPG 3400 Click External if an SRD 3x00 Solvent Rack is connected to the pump In all other cases click None Depending on the pump type Pump Device or Left Pump Device and Right Pump Device DGP 3600 Name The following names are the default names Pump Default Name Pump Device Name Left Pump Device Name Right Pump Device Name ISO 3100 HPG 3x00 LPG 3400 Pump n A n A DGP 3600 n A Pump...

Page 52: ...r the pump is purged through the membrane pump of the autosampler The default setting is None All pumps except DGP 3600 Click UM3WPS_PURGE0 to connect the pump fluidically with the autosampler Only DGP 3600 To connect both pumps of a DGP 3600 fluidically to the autosampler click UM3WPS_PURGE0 for one pump and UM3WPS_PURGE1 for the other pump To connect only one pump to the autosampler click UM3WPS...

Page 53: ...an HPG 3200SD Flow Shows the allowed flow range Enter a new value in the Minimum and or Maximum box to change the limits for the flow rate within the allowed range Pressure Shows the allowed pressure range Enter a new value in the Minimum and or Maximum box to change the pressure limits within the allowed range Click the arrow of the Pressure Unit box and select a different pressure unit psi bar o...

Page 54: ...ear for example in the gradient display of the online control panel and in the report Bottles page only DGP 3600 Check and change if necessary the settings for monitoring the solvent consumption and liquid level in the waste container Share Eluent Bottles The check box is selected by default Accept this setting if both pumps of the DGP 3600 are connected to the same solvent reservoirs If the check...

Page 55: ...hromeleon To change a signal name or the scaling factor overwrite the existing entry directly in the corresponding line To change the pressure unit make your selection on the Limits page The signal for the pump pressure Pump_Pressure is selected by default with a DGP 3600 Pump_PressureLeft and Pump_PressureRight Accept this setting if you want to record the pump pressure Chromeleon then generates ...

Page 56: ...s reopen the configuration pages 1 Start the Server Configuration program page 34 2 Right click the pump in the timebase and click Properties on the menu 3 Change the settings as needed For a description of the pages see section 3 5 3 1 page 35 4 To save the configuration click Save Installation on the File menu and then close the Server Configuration program ...

Page 57: ...luding details about the appropriate cabling and settings required in the Server Configuration program Only ESA Corona When the pump and detector are connected and configured as described in the Chromeleon Help pumping is stopped and an error message appears on the pump display and in the Chromeleon Audit Trail An emergency stop was requested over the digital input line if an error occurs in the d...

Page 58: ...UltiMate 3000 Series SD RS and BM Pumps Page 44 Operating Instructions ...

Page 59: ...3000 system includes an SRD 3x00 Solvent Rack Connect the 15 pin D Sub port on the rear of the Solvent Rack with the Solvent Rack port on the rear of the pump page 19 2 If you want to connect an ESA Corona or Coulochem III detector to the pump Refer to section 3 6 for details page 43 Before you attach the column and cell flush the system with several volumes of the mobile phase to be used 3 Connec...

Page 60: ... on the Devices page of the Properties dialog for the pump that the Degasser Control setting is correct page 36 Link the autosampler of the UltiMate 3000 system with the pump as appropriate page 80 8 Turn on the power to the pump page 62 9 Purge the pump page 54 10 Check and change the leak sensor setting if necessary page 86 11 Adjust the brightness and contrast of the pump display if necessary p...

Page 61: ...e fitting connection also observe the following Viper fitting connections Loosen and tighten the knurled screws of the viper capillaries only with your hand do not use tools First tighten the screw hand tight If the connection leaks tighten the screw a little more If leakage still exists replace the Viper capillary Capillaries with Viper fitting connections can be reused also for a different conne...

Page 62: ... the solvent reservoirs observe the following general precautions Always install filter frits on the solvent lines This prevents contaminants from reaching the HPLC system that may increase wear and cause damage to the system Regularly check the filter frits for permeability This is especially important when using aqueous solvents Aqueous solvents may contaminate the filters with algae and other m...

Page 63: ...normally not required to use a degasser with these eluents 4 3 2 Connecting the Solvent Reservoirs 4 3 2 1 All pumps except ISO 3100BM 1 Feed the solvent line through the retaining guide which holds the tubing in place in the reservoir and then into the open hole in the reservoir cap 2 Slide the filter holder with filter frit onto the end of the solvent line 3 If required Verify that the end of th...

Page 64: ...olvent reservoirs are located above the pump outlet during pump operation the hydrostatic pressure in the system may cause eluent to escape when you open a fluid connection in the pump Therefore before you open a fluid connection position the reservoirs below the connection to be opened 4 3 2 2 ISO 3100BM With an ISO 3100BM the solvent tubing is connected to the pump at the factory 1 Connect the f...

Page 65: ...er Fig 14 Peristaltic pump 2 Fill the liquid reservoir A reservoir is provided in the accessories kit of the pump Observe the precautions for the composition of the seal washing liquid on page 84 3 Connect the silicone tubing from the peristaltic pump with the liquid reservoir If necessary prolong the tubing for example by using part of the silicone tubing from the pump accessories kit 4 Place the...

Page 66: ...e left so that the liquid can easily pass the system c Reconnect the tubing to the detector Connect the tubing to the inner port Fig 15 page 51 The outer port has no function 7 Verify that the seal washing liquid is discharged properly to the waste section 4 5 For information about how to operate the pump with rear seal washing and for details about what happens see section 5 5 6 page 83 ...

Page 67: ...nage and detailed installation instructions Verify that the seal washing liquid is discharged properly to the waste 1 Remove the securing clip for the detector toward you and then remove the detector toward to top Fig 17 Detector of the seal wash system 2 Fill the connection port of the seal wash system several times with HPLC grade water Fig 18 until the liquid exits the drain port at the bottom ...

Page 68: ...mp 2 mL min 5 min If necessary you may change these standard settings in Chromeleon in the Commands dialog box or on the control panel for the pump or on the pump display on the Preferences menu page 75 Observe the following for the different pump types DGP 3600 The two pumps of a DGP 3600 must be purged separately ISO 3100 and HPG 3200 When the fluid components of the pump are filled with liquid ...

Page 69: ... of pressure drop needs to be connected to the pump outlet If necessary cap the pump outlet 5 Purge the pump from Chromeleon or from the pump display To purge the pump from Chromeleon a In Chromeleon open the Commands dialog box for the pump b Set the channel to be purged to 100 In Chromeleon you cannot set A directly Thus if A be 100 you have to set all other components of the eluent to 0 c Start...

Page 70: ...d PurgeTime has elapsed Purge is automatically reset to Off You can stop purging also manually by setting Purge to Off f Repeat the above steps for all channels of the pump even if they are not used for the application until all air bubbles are gone Tip With some pump types you can start the purge cycle also by selecting Purge on the navigation bar 6 Close the purge valve turn the valve knob clock...

Page 71: ...s page check the entry in the WPS Purge Port box to find out whether and with which autosampler the pump is fluidically connected page 38 3 In Chromeleon open the Commands dialog box for the pump page 65 and check and change the PurgeViaSampler setting if necessary Property Setting PurgeViaSampler Select Yes to purge the pump by using the autosampler when Purge On The property is listed under Pump...

Page 72: ...the expected reading for your application and is stable Perform system equilibration in Chromeleon or select the required commands and parameters on the front panel menus of the instruments To equilibrate the system from Chromeleon Select and perform the operating commands and parameters from the Commands dialog box Create and run an equilibration program to automate the process page 68 Use the Sm...

Page 73: ...m Fig 20 Equilibration panel To equilibrate the system from the front panel menus Select and perform the operating commands and parameters on the front panel menus of the instruments For information about the pump menus see section 5 4 2 page 72 For information about the menus of other system modules see the Operating Instructions for the respective module ...

Page 74: ...UltiMate 3000 Series SD RS and BM Pumps Page 60 Operating Instructions ...

Page 75: ...mp is powered up For a short time general information about the pump appears on the pump display device type serial number and bootloader and firmware versions The pump runs a series of internal tests During these self diagnostics all of the main components are checked When the self test was successful the initial screen changes to the status screen page 62 If an error is detected the pump is not ...

Page 76: ...ght Pump Device Name boxes On the pump display you can then select on the Control menu for which pump of the DGP 3600 the values are displayed page 75 Flow Pressure Components of the solvent in percent of the total flow In addition the following information may appear on the screen The screen shows When Running or Flow on The pump is delivering with the specified flow rate The display always shows...

Page 77: ...olvent composition Perform the Continue command in Chromeleon to cancel the Hold command For more information see the Chromeleon Help Stopped When you performed the StopFlow command for the pump from Chromeleon The retention time and the pump flow ware stopped Perform the Continue command in Chromeleon to cancel the StopFlow command For more information see the Chromeleon Help You can adapt the sc...

Page 78: ...connect the timebase in which the pump is installed to the Chromeleon client program section 5 3 1 Two modes of software control are available Direct control with the parameters and commands in the Commands dialog box page 65 or on a control panel page 67 Automated control with a control program PGM page 68 5 3 1 Connecting to Chromeleon 1 Start the Chromeleon Server Monitor and the Chromeleon ser...

Page 79: ... Commands dialog box for the pump 1 Open a control panel any panel is possible To open a control panel open the Chromeleon Browser and double click a control panel in the Dionex Templates Panels folder 2 Connect the control panel to the timebase in which the pump is installed On the Control menu select Connect to Timebase and then select the timebase on the Timebase tab For information about the T...

Page 80: ...evel if necessary Right click in the commands list and select the filter level on the menu Fig 22 Commands dialog box 6 Verify that the pump is connected to Chromeleon If it is not select Connect to connect the pump For a list of the commands and properties that are supported for the pump see the Chromeleon Help In addition to the pump commands and parameters the Commands dialog box provides acces...

Page 81: ...nels for performing system wide functions for example creating and running sequences For more information about panel tabsets see the Chromeleon Help 2 On the Panel Tabset for your timebase click the tab page for the pump for example Rapid Separation Pump for an RS pump Fig 23 Pump control panel 3 Verify that the pump is connected to Chromeleon the LED next to the Connect button is green If it is ...

Page 82: ...o create a program with the Program Wizard 1 Open the Program Wizard On the File menu select New and then select Program File 2 The wizard guides you through program creation On each wizard page make the desired settings or accept the default values For additional information about a page click Help 3 After you finish the wizard Chromeleon automatically creates the corresponding program 4 To start...

Page 83: ...he entire program listing the control commands in chronological order For more information see the Chromeleon Help 3 To start the program follow the steps in the next section To start a program Program for sample analysis 1 Create a sample list sequence A sequence must include the program and a method for evaluating the sample data for example for peak identification area determination and amount ...

Page 84: ...e spots on the front panel mark the positions of four function keys Menu Flow on or Flow off Set flow and Purge table further down in this section To show the keys touch the position of the utmost left spot on the pump display with the Dionex menu pen The menu pen is included in the pump shipment Fig 25 Showing the function keys The function keys replace the information in the bottom line of the s...

Page 85: ... delivering with the displayed flow rate the key name changes to Flow off the real flow is displayed and the flow value stops flashing Select Flow off to have the pump stop delivering Flow on Enter the pump flow Set flow Purge the pump The channel set on the Control menu page 75 is purged with the Purge flow and Purge time settings from the Preferences menu page 75 For more information about purgi...

Page 86: ... sections 5 4 2 2 through 5 4 2 6 page 74 1 Control 2 Preferences 3 Diagnostics Main 1 Self test 2 Compression right 3 Compression left 4 Workload right 6 Model 1 Flow 2 Flow rate 3 A 4 B 4 Configuration 1 Display soft keys 2 Leak sensor mode 3 Pressure unit 5 C 6 Max pressure 7 Min pressure 8 Selected pump 9 Purge 10 Change right pump pistons 11 Change left pump pistons 1 Degasser 2 Purge time 3 ...

Page 87: ...r parameter determines which keys appear on the navigation bar To Select Return to the previous entry on a list If the list contains 5 or more items you can use the arrow up key to scroll up through the list after reaching the first line Key autorepeat page 76 Increment numerical values Proceed to the next entry on a list If the list contains 5 or more items you can use the arrow down key to scrol...

Page 88: ... the menu structure To open the Main menu show the function keys and select Menu page 70 From the Main menu you can open the following menus Control On the Control menu you can make the different settings for pump operation page 75 Preferences On the Preferences menu you can make the basic settings for the pump page 75 Diagnostics The Diagnostics menu provides information for diagnostic purposes r...

Page 89: ...ump see section 4 6 page 54 Purge Open the Pump Maintenance dialog for piston and piston seal replacement From this dialog box you can undock the pistons for the replacement procedures and dock them later again For more information see section 7 5 2 page 135 Change right pump pistons Change left pump pistons Change pump pistons 5 4 2 4 Preferences Menu On the Preferences menu you can make the basi...

Page 90: ... See the serial number of the pump Serial number 5 4 2 6 Configuration Menu The Configuration menu provides information about the pump configuration and allows you to make the required settings and change the settings To Select Set the display and function key parameters Brightness sets the screen brightness The input is in percent Contrast sets the screen contrast The input is in percent Key soun...

Page 91: ... Chromeleon supports for operating the pump page 91 5 5 1 Choosing the Solvents Observe the following precautions The pump is primed with 2 propanol During initial operation of the pump make sure that the solvents used are miscible with 2 propanol Otherwise follow the appropriate intermediate steps Use only water of at least HPLC grade or better LC MS grade 0 2 µm filtered If water from water puri...

Page 92: ...onochlorobenzene If you use these solvents contact your Dionex sales representative If the UltiMate 3000 system includes an LPG 3400 pump or an SRD 3x00 Solvent Rack observe also the solvent compatibility of the degasser Whenever possible avoid using the following solvents hexafluoroisopropanol hydrofluorid containing solvents perfluorinated solvents and freons For modules with a serial number 801...

Page 93: ...sopropanol to replace them step by step After operation rinse out buffers and solutions that form peroxides Important You should not recycle the solvent or use methanol from aluminum reservoirs This may impair the performance of the seals Important Dionex déconseille de recycler les solvants ou employer du méthanol stocké dans des réservoirs en aluminium Ceci peut affecter les performances des joi...

Page 94: ...Configuration program page 34 2 Right click the autosampler in the timebase and click Properties on the menu 3 Click the Segments Pump Link page and select the pump to which the autosampler is linked Select the pump to which the autosampler is linked on the Flow through sampler is delivered by pump s list If the UltiMate 3000 system includes a DGP 3600 pump Select UM3PUMP_L_STRK to link the autosa...

Page 95: ... Commands dialog box for the pump page 65 2 Select Flow and enter the desired flow rate in the Nominal box The property is listed under PumpModule Pump Device Name page 37 The allowed flow rate range is indicated in the Properties dialog for the pump page 39 In the Properties dialog box you can change the upper and lower limit for the flow rate within the allowed range 3 Under MaximumFlowRampUp ch...

Page 96: ... setting is 1 MPa Upper Pressure Limit Helps to protect the column from too high a pressure The application and column type determine the setting To set the pressure limits in Chromeleon A Pressure range The range within which the pressure limits can be set and the pressure unit are specified in the in the Properties dialog for the pump page 39 You can set the upper and lower pressure limits only ...

Page 97: ...r Seal Wash System Rear seal washing helps avoiding damages to the pistons piston seals and support rings and thus increases the seal life For information about how to connect the rear seal wash system see page 50 5 5 6 1 Working with Rear Seal Washing The rear seal wash system is enabled and cannot be disabled As a standard one seal washing cycle is performed per hour However you can start an add...

Page 98: ...lity of the delivered solvent make the liquid slightly conductive by using the appropriate additives Do not use additives with a high salt content or additives that cause solid residuals upon evaporation Be sure that liquid drops are present in the detector 5 5 6 3 What happens Correct functioning of the rear seal wash system During the delivery period of the peristaltic pump liquid reaches the de...

Page 99: ...rrideRearSealDry to Enabled However note that the property will be automatically reset to Disabled after each wash cycle Possible leakage of the main piston seal If the message Piston seal leakage has exceeded the recommended limit appears this indicates possible leakage of the piston seals Take the following remedial action Inspect the piston seals for leakage page 134 Consider replacing the pist...

Page 100: ...ate the source for the leak eliminate the cause and dry the leak sensor page 124 You can disable the alarm temporarily To do so open the Commands dialog box for the pump and perform the AlarmOff command This also turns off the beep and allows you to restart the pump flow If the leak sensor does not report Leak NoLeak within 30 minutes after you have restarted the flow another leak alarm will be is...

Page 101: ...or the screen contrast as required 5 5 10 SmartStartup and SmartShutdown The SmartStartup wizard assists you in automating regular routine tasks page 58 With SmartStartup the different modules of the UltiMate 3000 system are turned on automatically and in a controlled manner For example SmartStartup can purge the pump of the HPLC system automatically flush the column and perform system equilibrati...

Page 102: ...es and rinse the degassing channels at regular intervals page 157 Dionex advises against recycling the solvents This may impair the degassing performance Before connecting the solvent lines make sure that the connectors are free of contaminants Even minute particles can allow air to enter the degasser and thus reduce the degassing effectiveness When replacing solvents make sure that the solvents a...

Page 103: ...0 Solvent Rack is connected to the pump page 36 3 Open the Commands dialog box for the pump and set Degasser to On or Off or Open the Panel Tabset for the pump page 67 and click More Options Under Degasser set Mode to On or Off When the degasser is operated in Chromeleon monitoring of the degasser vacuum and leak detection is performed from the pump The related commands and properties are availabl...

Page 104: ...ge valve Damages to the pulse damper may result To prevent eluent from suddenly escaping any connections open any capillary connections in the pump and on to the column only when the system pressure is almost down Wait until the pressure is less than 1 MPa before opening a connection If the pressure can be reduced only by opening the purge valve turn the valve knob slowly counterclockwise to allow...

Page 105: ...tion see the Chromeleon Help 5 6 1 Predictive Performance Predictive Performance provides various functions for estimating the lifetime of consumables and for monitoring and recording service and re qualification information Commands Dialog Box Open the Commands dialog box for the pump and enter the limits for the predictive performance parameters For a list of the commands and counters that are s...

Page 106: ...e control panel for the pump click Wellness Qualification and Service to see the related predictive performance commands and parameters on separate panels On these panels you can enter the limits and reset the counters In addition wellness bars provide visual indicators of qualification and service periods The color coding of the wellness bars provides information about the status Color Descriptio...

Page 107: ... performance of the pump that is the pressure pulsation and compressibility compensation Performance Test The permeability of the static mixer or inline filter Mixer Frit Test Degasser vacuum You can run this test for both the internal degasser of an LPG 3400 pump and the degasser of an SRD 3x00 Solvent Rack Degasser Vacuum Test Before running these tests be sure that the StaticMixer property is s...

Page 108: ...itoring the liquid level in the waste container The following properties are available for example in the Commands dialog box for the pump Solvent Reservoirs X A B C or D Description X_Level Enter the following information for the related component of the solvent Value The volume at the beginning of the sequence LowerLimit The lower limit If the liquid level in the solvent reservoirs reaches the l...

Page 109: ... upper limit x warning limit 100 WasteLevel UpperLimit x WasteWarningLimit 100 WasteRemain Time Reports the time left until the liquid level is expected to reach the upper limit The time is calculated from the current flow rate and the calculated current liquid level For more information about the properties in the table and about emergency programs see the Chromeleon Help Only DGP 3600 In the Pro...

Page 110: ...d in the Properties dialog for the pump page 41 When these conditions are fulfilled the relay outputs and digital inputs are available in Chromeleon for example in the Commands dialog box for the pump and can be programmed as required For information about the functions of the connector pins and pin assignment see page 215 5 6 6 Operational Qualification and Performance Qualification Operational Q...

Page 111: ...thanol If the solvents in the pump are not miscible with water use an appropriate intermediate solvent Rinse out buffers or solvents that form peroxide Turn off the lamps in any UV or RF detectors that are connected to the pump This will prevent evaporation of the solvents in the flow cell When using an electrochemical EC detector with the pump always turn off the potential to the EC cells before ...

Page 112: ...o create a standby or shutdown program Select one of the following alternatives Select and perform the operating commands and parameters from the Commands dialog box page 65 Create and run a corresponding program to automate the process page 68 Use the SmartShutdown Wizard to create and run the program see the next section To create the program with the SmartShutdown wizard 1 To open the wizard cl...

Page 113: ... functions for estimating the lifetime of consumables and diagnostic tests to check the performance of certain pump components pages 91 and 93 ISO 3100 and HPG 3200 When the fluid components of the pump are filled with liquid and the solvent reservoirs are located above the pump outlet during pump operation the hydrostatic pressure in the system may cause eluent to escape when you open a fluid con...

Page 114: ...hen using aqueous solvents This is especially important with aqueous solvents Aqueous solvents may contaminate the filters with algae and other microorganisms that deposit on the filter frits Therefore also replace the solvents at regular intervals Rinse the reservoirs thoroughly before refilling them Check the drain line connected to the drain port at the bottom right of the pump Verify that the ...

Page 115: ...e for the pumps Maintenance kit for Part No ISO 3100SD 6040 1950 ISO 3100BM 6042 1950 HPG 3x00SD 6040 1953 HPG 3x00RS 6040 1956 LPG 3400SD 6040 1951 LPG 3400RS 6040 1954 LPG 3400BM 6042 1951 DGP 3600SD 6040 1952 DGP 3600RS 6040 1955 DGP 3600BM 6042 1952 For information about the kit content see section 11 3 page 199 ...

Page 116: ...UltiMate 3000 Series SD RS and BM Pumps Page 102 Operating Instructions ...

Page 117: ...message appears on the pump display Check the Messages on the Pump Display section for recommended courses of action page 105 If the pump is operated with Chromeleon a message is also displayed in the Chromeleon Audit Trail Tip For information about operating problems that might occur during the operation of the pump or an UltiMate 3000 system see Operating Problems page 114 Diagnostics tests If t...

Page 118: ...everal strokes afterward the message Compression back to normal will appear on the display For more information about the compression values see section 6 6 page 121 During pump maintenance The pistons are in the appropriate position for piston seal replacement The LED flashes red The pistons are not in the appropriate position for normal operation for example after piston seal replacement or an e...

Page 119: ... with Chromeleon consider also checking the Audit Trail message The Audit Trail may provide additional information In addition to the messages listed in the table other messages may appear In this case note the exact wording of the message and contact Dionex Service if you are unable to eliminate the problem Error Message Remedial Action x counts deviation in zero position where x is the exact dev...

Page 120: ...an 100 for several strokes afterward the message Compression back to normal will appear on the display For more information about the compression values see section 6 6 page 121 Configuration error Found x ID chips and y pump blocks where x and y are numbers A pump block is defective Contact Dionex Service Degasser malfunction LPG 3400 The vacuum level monitoring function of the degasser in the pu...

Page 121: ...rn the pump off and on again by pressing the power switch on the rear of the module If the message appears again contact Dionex Service Pressure buildup during maintenance operation Please open the purge valve A pressure of more than 0 5 MPa built up during piston and or piston seal maintenance Open the purge valve to reduce the pressure If you are operating an ISO 3100BM observe the precautions o...

Page 122: ...There is a leak in the system or a fluid connection is loose Find and eliminate the source for the leak Tighten leaking connections and dry the leak sensor Operating Instructions for the Solvent Rack The cam position is yet unknown It is necessary to run at least 2 revolutions The position of the cam is unknown after the piston has been replaced Turn on the flow and have the pump run for two revol...

Page 123: ...nother Wash cycle manually To do so set RearSealWashPump to Active page 83 Inspect the rear seal wash tubing for indications of leakage page 125 Verify that the rear seal tubing is connected properly Fig 4 page 21 The peristaltic tubing may be blocked or draws air Replace the tubing if necessary Check the tubing for permeability Clean the detector of the rear seal wash system page 127 Make sure th...

Page 124: ...f range Verify that the pressure transducer cable of the pump head is properly connected to the P Work connector on the interior front panel Zero position not found during the last revolution Turn the pump off and on again by pressing the power switch on the rear of the pump If the message appears again contact Dionex Service Zero position not found during the last 3 revolutions Turn the pump off ...

Page 125: ...wer supply to the pump may be interrupted Check the power supply connection of the pump Error issuing control request to HPG 3400 USB 1610103 The USB connection between the pump and the Chromeleon server may be interrupted Check the USB connection Check the power supply connection of the pump Remove the pump specified in the message from the server configuration or else select a different pump fro...

Page 126: ... page 147 or inline filter page 149 LPG 3400BM and DGP 3600 BM Replace the inline filter page 150 or the frit in the inline filter page 151 General Leak Test failed Probable Cause Remedial Action The pressure drop has been too high The pressure level has not been reached in time The initial pressure has not been reached within the first minute The pressure drop during relaxation has been too high ...

Page 127: ...roblems with the restrictor The restrictor may be blocked Consider replacing the restrictor The pressure was outside specification The pressure was outside the upper and lower pressure limits Repeat the test with changed flow settings The compression value was over the limit There may be air bubbles in the pump head Purge the pump page 54 The flow may have been too high Repeat the test with reduce...

Page 128: ...th Chromeleon There is no connection between the pump and the Chromeleon computer Check the USB cable and connection to the computer The USB port on the computer is not ready for operation Check the USB port on the computer The rear seal wash system is leaking The seal washing tubing is not connected properly the tubing is damaged by bending or is blocked Check the seal wash tubing Fig 4page 21 Th...

Page 129: ... contaminated or blocked Rinse or replace the column High baseline drift The column is contaminated Clean or replace the column The system is not sufficiently equilibrated Flush the system until equilibration The solvent is degraded Use fresh solvent and check the eluent filter frits In aqueous solvents growth of microorganisms is possible The environmental conditions are unstable Make sure that t...

Page 130: ...is degraded Use fresh solvent There are pressure fluctuations from the pump Purge the pump page 54 Check the compression values for the pump and perform the remedial actions as suggested page 121 There are air bubbles in the system Purge the pump page 54 The inlet valve or outlet valve on the working cylinder is dirty Clean the inlet check valve and or outlet check valve Consider replacing the che...

Page 131: ...uilibration time after the flush cycle is too short or the dead volume is too high Increase the equilibration time or eliminate possibly existing dead volume Poor reproducibility The autosampler draws air from the vial There is not enough amount of sample in the vial the needle height setting is incorrect Autosampler Manual or there are too many replicates There are air bubbles in the syringe Flus...

Page 132: ...y connections Replace the fittings Make sure that the capillaries are connected correctly The piston seals are leaking Replace the piston seals page 135 There is air in the working cylinder Purge the pump page 54 There is pump pulsation Use degassed solvents Check the compression value for the pump head page 128 The gradient is irreproducible Change the gradient Check the pump function and degassi...

Page 133: ...he inline filter page 151 There are air bubbles in the flow path Perform a wash cycle Autosampler Manual Non degassed wash liquid is used Degas the wash liquid Autosampler manual After a maintenance procedure replacement of pump head piston or piston seal the pistons are not in the appropriate position for normal operation Remove and reinstall the pistons pages 135 and 137 Flow fluctuation The inl...

Page 134: ...ndications for leakage Check the degasser vacuum The flow rate is too high Reduce the flow rate Reproducible ghost peaks in the chromatogram The degassing channels are contaminated Rinse the degassing channels page 157 or Solvent Rack manual The solvents are degraded or dirty or their purity is insufficient Use fresh and appropriate solvents Contamination occurs somewhere in the system Flush the s...

Page 135: ...er than 100 and remain stable If the value is close to 100 the required precompression may not be reached and pulsation may occur The table shows guides values for some solvents Solvent Compression RS pumps SD pumps BM pumps Acetonitrile 45 10 70 10 75 10 Methanol 50 10 70 10 80 10 Water 25 10 50 10 55 10 with 100 of degassed solvent delivered at 40 MPa pump purged and stable for some time The val...

Page 136: ...UltiMate 3000 Series SD RS and BM Pumps Page 122 Operating Instructions ...

Page 137: ... to escape when you open a fluid connection in the pump Therefore before you open a fluid connection position the reservoirs below the connection to be opened Use original Dionex spare parts only Substituting non Dionex parts or using non Dionex accessories may impair the performance of the pump thereby voiding the product warranty For more information see the warranty statement in the terms of sa...

Page 138: ...king connections 3 With a cloth or tissue absorb all liquid that has collected in the tray Make sure that you do not bend or damage the sensor 4 Allow the sensor to adjust to the ambient temperature for a few minutes The red sensor LED should now be dark The yellow LED may be dark or illuminated this LED does not indicate errors 5 If no errors are reported operation can be resumed 6 Do not forget ...

Page 139: ... syringe at the open end of the tubing Press the lever of the peristaltic pump to the left so that the liquid can easily pass the system 3 Press the lever of the peristaltic pump firmly onto the tubing and then press liquid into the seal wash system from the syringe 4 Inspect the tubing and connections of the seal wash system for indications of leakage Tighten or replace leaking connections as nec...

Page 140: ... tubing 4 Locate the Pharmed tubing white tubing in the Tubing Kit for the Rear Seal Wash System and cut the tubing to the required length 5 Connect the new tubing to the connection port on the pump head and to the tubing from the seal wash reservoir Push the Pharmed tubing onto the connector as far as it goes on a small collar should be visible 6 Engage the tubing in the peristaltic pump Press th...

Page 141: ... toward to top 4 Install the new detector following the above steps in the reverse order Connect the tubing to the inner port Fig 30 The outer port has no function 7 3 4 Cleaning the Detector The detector can be contaminated by deposits impairing the correct functioning of the seal wash system 1 Remove the detector as described in section 7 3 3 2 Clean the detector with distilled water and wait un...

Page 142: ...mp flow rate to 0 Wait until the system pressure is down to zero 3 Put on a pair of clean room gloves to prevent contamination on the valve parts Even minute particles may cause damage to the system and result in poor pump performance 4 Disconnect the tubing connections from the inlet and outlet check valves 5 Loosen the check valve nuts by using a 13 mm open end wrench and remove them from the pu...

Page 143: ...itting a little more Do not overtighten this will crush the cartridge 10 To prevent contaminants from entering the HPLC system thoroughly rinse the pump using at least 30 mL HPLC grade water or purely organic solution Open the purge valve with an ISO 3100BM observing the precautions on page 90 to prevent the rinsing liquid from entering the HPLC system 11 Test the pump for leakage page 154 Tighten...

Page 144: ...tions 7 5 Pistons and Piston Seals Fig 34 Pump head piston and piston seals here for a HPG 3x00 Depending on the pump version the design of some parts may differ from the representation in the picture However the position of the parts is the same for all pump versions ...

Page 145: ...g of the piston seal 6040 0012 2 rings 7 Piston seal reversed phase 6040 0304 2 seals 8 Seal ring rear seal wash system Included in 6040 2208 9 Pump Head 10 Valve cartridge same as no 12 6041 2301 6041 2300 11 Valve nut inlet check valve Included in 6040 7007 12 Valve cartridge same as no 10 6041 2301 6041 2300 13 Valve nut outlet check valve Included in 6040 7007 14 Tubing connector for the seal ...

Page 146: ...ing 6 Support ring of the piston seal 6040 0012 2 rings 7 Piston seal reversed phase 6040 0304 2 seals 8 Seal ring rear seal wash system Included in 6040 2208 9 Pump Head 10 Valve cartridge same as no 12 6041 2301 6041 2300 11 Valve nut inlet check valve Included in 6040 7007 12 Valve cartridge same as no 10 6041 2301 6041 2300 13 Valve nut outlet check valve Included in 6040 7007 14 Tubing connec...

Page 147: ...hing 6 Support ring of the piston seal Included in 6025 2012 7 Piston seal reversed phase Included in 6025 2012 8 Seal ring rear seal wash system Included in 6040 2208 9 Pump Head 10 Valve cartridge same as no 12 6041 2300 11 Valve nut inlet check valve Included in 6042 7007 12 Valve cartridge same as no 10 6041 2300 13 Valve nut outlet check valve Included in 6042 7007 14 Tubing connector for the...

Page 148: ...wash cycle starts If the level remains unchanged the piston seals seal tightly Proceed with the next step A rising level indicates leakage from one or more piston seals Follow these steps a On the right pump head remove the seal wash tubing connecting the left pump head to the right pump head b Repeat the observation c If the level rises again replace the piston seals in the left pump head page 13...

Page 149: ... the pump head can be removed before you continue with the next steps or In Chromeleon perform the UndockPistons command from the Commands dialog box listed under Pump Device Name _Wellness_RightBlock and or Pump Device Name _Wellness_LeftBlock for information about the Pump Device Name see page 37 Wait until the PistonPositionStatus property reports Undocked before you continue with the next step...

Page 150: ...ogether with the pump head Pull the pistons out of the pump head It may happen that the plate of the rear seal wash system remains in the pump when you remove the pump head If it does set the pump head tool to the plate as shown in the picture and carefully remove the plate from the pump block The pump head tool is provided in the accessories kit of the pump Remove the pistons from the pump Fig 37...

Page 151: ...cessories kit of the pumps Place the spacing tool on the plate of the rear seal wash system and push both pistons downward as far as possible The distance is correct when both pistons touch the spacing tool Remove the tool Fig 38 Establishing the distance 3 Place the assembly back into the pump and tighten the pump head screw with an Allen wrench size 6 mm recommended torque 10 Nm If both pump hea...

Page 152: ...ump Maintenance screen on the pump display select Dock Wait until the screen returns to the Main menu before continuing with the next steps or In Chromeleon perform the DockPistons command from the Commands dialog box listed under Pump Device Name _Wellness_RightBlock and or Pump Device Name _Wellness_LeftBlock for information about the Pump Device Name see page 37 Wait until the PistonPositionSta...

Page 153: ...137 6 After you have replaced the piston seals observe the recommendations on page 144 If you want to replace the piston seals in the plate of the rear seal wash system follow the steps on page 145 Tips The pump is shipped with reversed phase piston seals RP Keep in mind that using chloroform trichlorobenzene methylene chloride tetrahydrofuran or toluene as solvents chemically damages the UHMW PE ...

Page 154: ... Tap the pump head on a surface to make the support ring drop out If this does not remove the support ring push the seal insertion tool into the pump head and remove the tool The support ring is removed together with the tool Tip Do not use the seal removal tool for the support ring as the ring can be removed from the tool only with difficulties 5 Remove the piston seal from the pump head Push the...

Page 155: ...ming the fingernail test in addition Hold the piston and carefully move your fingernail over the surface as shown in the picture After performing this test you have to clean the piston again The piston is clean when the surface is completely smooth no inconsistent or rough areas Fig 41 Fingernail test 1 Carefully rinse the piston preferably with isopropanol and then rub it several times with a dry...

Page 156: ...performance 1 Depends on the pump type RS and SD pumps With these pumps the support ring is not a wear part Thus you need not replace the support ring when you replace the piston seal However before reinstalling the support ring you should clean the support ring from the inside and outside using isopropanol a cleaning swab part no 6040 0006 and a lint free cloth or tissue BM pumps With these pumps...

Page 157: ...on of the rear seal wash body 7 Place the plate of the rear seal wash system onto the pump head as shown in the picture The channels in the plate are facing you Fig 45 Plate of the rear seal wash system installed 8 Install the pistons and pump head page 137 9 After replacing the piston seals observe the recommendations on page 144 10 Update the related service information in Chromeleon for the pum...

Page 158: ...wise e On the pump outlet install a flow restrictor for example a capillary that can generate approximately 30 MPa at a flow rate of 1 mL min f Have the pump deliver isopropanol at 1 mL min for another 30 minutes g Depending on the pump type check the static mixer or inline filter for indications of blockage page 148 or 152 h Remove the capillary from the pump outlet and connect the pump to the sy...

Page 159: ...hown in the picture and push the seal out of the plate Fig 46 Removing the seal from the plate of the seal wash system 4 Insert and push the new seal into the plate high pressure side with your hand To prevent contamination put on a pair of gloves Mind the correct orientation of the seal The open side of the seal must face away from the plate Fig 47 Inserting the piston seal into the plate of the ...

Page 160: ... Volume 50 µL for HPG 3x00 SD and RS LPG 3400 SD and RS DGP 3600 SD and RS 6040 3015 6040 3026 6040 3025 Tip When you loosen or tighten capillaries on the static mixer hold and fix the static mixer with the open end wrench size 13 mm to prevent the mixer from turning in the mounting bracket The open end wrench is provided in the accessories kit for the pump 1 Disconnect the capillary mixer on the ...

Page 161: ...e open end wrench size 13 mm to prevent the mixer from turning in the mounting bracket The open end wrench is provided in the accessories kit for the pump 1 Disconnect the capillaries on the inlet and outlet of the static mixer Fig 49 Static mixer here in an HPG 3x00SD 2 Remove the static mixer from the mounting bracket by pulling the mixer toward you 3 Insert the new mixer into the bracket and re...

Page 162: ...ability of the inline filter also by running the Mixer Frit Test from Chromeleon page 93 1 Disconnect the capillary on the outlet of the static mixer 2 Have the pump deliver water at a flow rate of 2 mL min When the outlet is open the pressure should be less than 0 5 MPa 3 If required Replace the static mixer page 147 4 Reconnect the capillary to the outlet of the static mixer 5 Test the pump for ...

Page 163: ...13 mm to prevent the filter from turning in the mounting bracket 1 Disconnect the capillary on the filter inlet and filter outlet Fig 50 Inline filter 2 Remove the inline filter from the mounting bracket by pulling the filter toward you 3 Insert the new inline filter into the bracket and reconnect the capillaries to the filter inlet and outlet 4 After you have replaced the inline filter update the...

Page 164: ...199 and following pages 1 Disconnect the capillaries on the filter inlet and outlet and remove the inline filter from the pump Fig 51 Inline filter 2 Connect the capillaries to the new inline filter observing the direction of flow through the filter as indicated by an arrow on the filter and place the filter below the pump head in the pump enclosure 3 After you have replaced the filter update the ...

Page 165: ...scribed in section 7 7 2 1 2 Open the inline filter and remove the filter frit 3 Pipette a drop of solvent into the frit holder and insert the new frit Close the filter housing The solvent keeps the frit in the holder during closing 4 Install the inline filter as described in section 7 7 2 1 5 Test the pump for leakage page 154 6 After you have replaced the filter frit update the related service i...

Page 166: ...e Mixer Frit Test from Chromeleon page 93 1 Disconnect the capillary on the filter outlet 2 Have the pump deliver water at a flow rate of 2 mL min When the outlet is open the pressure should be less than 0 5 MPa 3 If required ISO 3100 Replace the inline filter page 149 LPG 3400BM or DGP 3600BM Replace the inline filter page 150 or the frit in the filter page 151 4 Reconnect the capillary to the ou...

Page 167: ...wise all the way and then pull it straight off the purge unit 4 Before installing the new purge valve knob consider cleaning the hole in the purge unit with a cleaning swab part no 6040 0006 5 Hold the new purge valve knob carefully You should not hold the knob by the seal end to avoid scratching the seals These scratches will prevent proper seal and allow leakage Fig 52 Purge valve knob 6 Insert ...

Page 168: ...SO 3100BM Connect the restrictor included in the Diagnostics Tool Kit part no 6040 3099 to the pump outlet and close the other end of the capillary with a fitting plug Appropriate fitting plugs are provided in the accessories kit of the pump 2 In Chromeleon set the upper pressure limit under Pump Device Name Pressure UpperLimit For information about the Pump Device Name see page 37 Pump Type Upper...

Page 169: ...of leakage and tighten leaking connections Piston seals Inspect the piston seals for leakage page 134 Consider replacing the piston seals page 135 If leakage is observed with new piston seals operate the pump for at least 2 hours at 35 MPa to run in the seals In this case tightening the pump head screw does not help Check valves Tighten the valve nuts page 128 Remove the valve cartridges page 128 ...

Page 170: ...if the pressure drops at the same rate as before If the pressure drops significantly slower the connection was leaking 8 In Chromeleon reset the upper pressure limit to the value used before the leak test Tip If leakage from a pump head is observed check also the tubing connected to the rear seal wash system If the seal wash tubing is not connected properly or if the tubing is crimped or kinked th...

Page 171: ...e In persistent cases for example reproducible ghost peaks in the chromatogram follow these steps 1 Install a backpressure capillary on the pump outlet The capillary should be appropriate for generating a backpressure of 20 to 30 MPa 2 Rinse the degassing channels for 1 hour with about 20 nitric acid at the flow rate normally used for your application Use fresh HPLC grade water 3 Rinse the degassi...

Page 172: ...Assurez vous de bien débrancher le cordon d alimentation de la source secteur 1 Remove the fuse cartridge by using a small screwdriver Fig 53 Fuse cartridge 2 Replace the fuses Dionex recommends always replacing both fuses Description Part No Fuse 2A slow blow 5 x 20 mm Included in the Fuses Kit part no 6030 9003 For information about the kit see section 11 3 page 199 3 Reinstall the fuse cartridg...

Page 173: ...firmware from Chromeleon this information will also be provided during the download see later in this section Whenever a new firmware version is released for the pump the new version will be provided with the next Chromeleon Service Release and described in the related release notes The new firmware will not be downloaded automatically to the pump when you install a Chromeleon Service Release To u...

Page 174: ...ngrade the firmware Older firmware may be incompatible with new hardware revisions 6 Click Yes to start the download Click No to cancel the action The download may take several minutes The download is complete when Firmware download completed successfully appears in the Messages Server window in the Chromeleon Server Configuration program The message appears also in the Chromeleon Audit Trail Imme...

Page 175: ...ating principle schematics 162 163 164 ISO 3100BM Interior components Liquid flow path Operating principle schematics 165 166 167 LPG 3400 Interior components Liquid flow path Operating principle schematics 168 169 171 DGP 3600 Interior components Liquid flow path Operating principle schematics 172 173 175 HPG 3200 Interior components Liquid flow path Operating principle schematics 176 177 178 HPG...

Page 176: ... Description 1 Peristaltic pump 2 Detector of the rear seal wash system 3 Capillary guides 4 Pump head with working cylinder and equilibration cylinder 5 Leak sensor 6 Inline filter page 149 7 Purge unit with purge valve and pressure transducer for the system pressure 8 Pump lights here hidden by the front panel door 9 Pump block status LED page 104 7 1 2 3 4 3 5 8 9 6 ...

Page 177: ...l degasser to pump head 6030 2546 2 Pump head with working cylinder and equilibration cylinder entire assembly section 7 5 page 130 3 Capillary from working cylinder to equilibration cylinder U tube Included in 6040 3001 4 Capillary from pump head to purge unit Included in 6040 3001 5 Purge unit with purge valve and system pressure transducer 6 Capillary from purge unit to inline filter 6040 3024 ...

Page 178: ... illustrates how the pump operates Fig 56 Operating principle of the ISO 3100SD No Element 1 Pump head with working cylinder no 1a and equilibration cylinder no 1b 2 Purge unit with purge valve knob no 2a and outlet nozzle no 2b 3 Inline filter 4 Pump outlet For general information about the operating principle see page 15 ...

Page 179: ...eristaltic pump 2 Detector of the rear seal wash system 3 Capillary guides 4 Pump head with working cylinder and equilibration cylinder 5 Leak sensor 6 Pulse damper 7 Inline filter page 149 8 Purge unit with purge valve and pressure transducer for the system pressure 9 Pump lights here hidden by the front panel door 10 Pump block status LED page 104 6 8 1 2 3 4 3 5 9 10 7 ...

Page 180: ...030 2546 6030 2548 2 Pump head with working cylinder and equilibration cylinder entire assembly section 7 5 page 130 3 Capillary from working cylinder to equilibration cylinder U tube Included in 6042 3002 4 Capillary from pump head to purge unit Included in 6042 3002 5 Purge unit with purge valve and system pressure transducer 6 Capillary from purge unit to pulse damper Included in 6042 3002 7 Pu...

Page 181: ...tes how the pump operates Fig 59 Operating principle of the ISO 3100BM No Element 1 Pump head with working cylinder no 1a and equilibration cylinder no 1b 2 Purge unit with purge valve knob no 2a and outlet nozzle no 2b 3 Pulse damper 4 Inline filter 5 Pump outlet For general information about the operating principle see page 15 ...

Page 182: ...eak sensor 6 LPG 3400SD and RS Capillary mixer page 22 LPG 3400BM Capillary from purge unit to inline filter 7 4 channel vacuum degasser 8 Pump lights here hidden by the front panel door 9 LPG 3400SD and RS Static mixer page 22 LPG 3400BM Inline filter 10 Purge unit with purge valve and pressure transducer for the system pressure 11 Pump block status LED page 104 12 4 channel proportioning valve 1...

Page 183: ...000 Series SD RS and BM Pumps Operating Instructions Page 169 8 2 2 Flow Path The picture illustrates the liquid flow path through the pump Fig 62 Flow path through an LPG 3400 top LPG 3400SD RS bottom LPG 3400BM ...

Page 184: ...linder and equilibration cylinder entire assembly section 7 5 page 130 7 Capillary from working cylinder to equilibration cylinder U tube LPG 3400RS and LPG 3400SD LPG 3400BM Included in 6040 3001 Included in 6042 3001 8 Capillary from pump head to purge unit LPG 3400RS and LPG 3400SD LPG 3400BM Included in 6040 3001 Included in 6042 3001 9 Purge unit with purge valve and system pressure transduce...

Page 185: ...gasser 2 Proportioning valve 3 Pump head with working cylinder no 3a and equilibration cylinder no 3b 4 Purge unit with purge valve knob no 4a and outlet nozzle no 4b 5 6 LPG 3400SD and LPG 3400RS Two step mixing system with capillary mixer no 5 and static mixer no 6 LPG 3400BM Capillary from purge unit to inline filter no 5 and inline filter no 6 7 Pump outlet For general information about the op...

Page 186: ...inder 5 LPG 3600SD and RS Capillary mixer page 22 LPG 3600BM Capillary from purge unit to inline filter 6 Purge unit with purge valve and pressure transducer for the system pressure 7 Leak sensor 8 Pump lights here hidden by the front panel door 9 Pump block status LED page 104 10 DGP 3600SD and RS Static mixer page 22 LPG 3600BM Inline filter 11 3 channel proportioning valve 9 1 2 3 4 3 7 8 10 5 ...

Page 187: ...000 Series SD RS and BM Pumps Operating Instructions Page 173 8 3 2 Flow Path The picture illustrates the liquid flow path through the pump Fig 66 Flow path through a DGP 3600 top DGP 3600SD RS bottom DGP 3600BM ...

Page 188: ... section 7 5 page 130 5 Capillary from working cylinder to equilibration cylinder U tube DGP 3600RS and DGP 3600SD DGP 3600BM Included in 6040 3001 Included in 6042 3001 6 Capillary from pump head to purge unit DGP 3600RS and DGP 3600SD DGP 3600BM Included in 6040 3001 Included in 6042 3001 7 Purge unit with purge valve and system pressure transducer 8 DGP 3600RS and SD Capillary mixer page 146 DG...

Page 189: ...ation cylinder no 8b 3 Purge unit with purge valve knob no 3a and outlet nozzle no 3b 9 Purge unit with purge valve knob no 9a and outlet nozzle no 9b 4 5 DGP 3600SD and DGP 3600RS Two step mixing system with capillary mixer no 4 and static mixer no 5 10 11 DGP 3600SD and DGP 3600RS Two step mixing system with capillary mixer no 10 and static mixer no 11 DGP 3600BM Capillary from purge unit to inl...

Page 190: ...eristaltic pump 2 Detector of the rear seal wash system 3 Capillary guides 4 Pump head with working cylinder and equilibration cylinder 5 Leak sensor 6 Purge unit with purge valve and pressure transducer for the system pressure 7 Pump lights here hidden by the front panel door 8 Pump block status LED page 104 9 Capillary mixer page 22 10 Static mixer page 22 1 2 4 10 3 4 6 3 5 7 7 8 8 9 ...

Page 191: ...ines 6035 2530 2 Pump head with working cylinder and equilibration cylinder entire assembly section 7 5 page 130 3 Capillary from working cylinder to equilibration cylinder U tube Included in 6040 3000 4 Capillary from left pump head to purge unit Included in 6040 3000 5 Purge unit with purge valve and system pressure transducer 6 Capillary mixer page 146 7 Static mixer page 147 8 Capillary from r...

Page 192: ...of the HPG 3200 No Element No Element 1 1a 1b Left pump head with working cylinder equilibration cylinder 3 3a 3b Purge unit with purge valve knob outlet nozzle 4 5 Two step mixing system with capillary mixer no 4 and static mixer no 5 2 2a 2b Right pump head with working cylinder equilibration cylinder 6 Pump outlet For general information about the operating principle see page 15 ...

Page 193: ...ctor of the rear seal wash system 3 Capillary guides 4 Pump head with working cylinder and equilibration cylinder 5 Leak sensor 6 Purge unit with purge valve and pressure transducer for the system pressure 7 Pump lights here hidden by the front panel door 8 Pump block status LED page 104 9 Solvent Selector 2 from 4 page 181 10 Capillary mixer page 22 11 Static mixer page 22 1 2 3 4 4 6 3 9 5 10 11...

Page 194: ... 3 Tubing from solvent selector to pump head set of 2 6040 3017 4 Pump head with working cylinder and equilibration cylinder entire assembly section 7 5 page 130 5 Capillary from working cylinder to equilibration cylinder U tube Included in 6040 3000 6 Capillary from left pump head to purge unit Included in 6040 3000 7 Purge unit with purge valve and system pressure transducer 8 Capillary mixer pa...

Page 195: ... Solvent B Solvent C Solvent D In a specific time step each pump head delivers only one of the two channels 100 It is not possible to specify a mixing ratio for the two channels connected to the same pump head A and C B and D Before you can enter a value for B you have to set D to 0 The same applies to all other channels Example 1 If you enter a portion for solvent C while solvent A is already set...

Page 196: ...181 2 Left pump head with working cylinder no 2a and equilibration cylinder no 2b 3 Solvent Selector 4 Right pump head with working cylinder no 4a and equilibration cylinder no 4b 5 Purge unit with purge valve knob no 5a and outlet nozzle no 5b 6 7 Two step mixing system with capillary mixer no 6 and static mixer no 7 8 Pump outlet For general information about the operating principle see page 15 ...

Page 197: ...For information about the gradient delay volume of the different pump types see the Technical Information section page 187 For gradient separations at low flow rates for example less than 100 µL min or very steep gradients you can adapt the gradient delay volume to your requirements by replacing the two step mixing system with which the pump is shipped with either a two step mixing system with low...

Page 198: ...tails see Mixing systems with a mixing volume 100 µL under this table If you want to replace the mixing system with which the pump is shipped page 22 with a system mentioned in the table observe the following Mixing systems with a mixing volume 100 µL For these mixing systems you need not replace the capillary mixer The capillary mixer is the same in both mixing systems and thus the mixer is not i...

Page 199: ...llary mixer on the purge unit 3 Connect the new capillary mixer 25 µL to the purge unit 4 Connect the free end of the capillary mixer with the inline filter and place the inline filter below the right pump head in the pump enclosure Fig 74 Single step mixing system with inline filter here in an HPG 3x00 5 Connect an appropriate capillary for the connection to the autosampler on the mixer outlet 6 ...

Page 200: ...UltiMate 3000 Series SD RS and BM Pumps Page 186 Operating Instructions ...

Page 201: ... 16 x 42 x 51 cm h x w x d PC connection All functions controllable via USB USB 1 1 or 2 0 Integrated USB hub with three USB ports USB 1 1 or 2 0 I O Interfaces 2 digital inputs 2 relay outputs 15 pin D Sub port for connection of a Solvent Rack or degasser Safety features Leak sensor active rear seal wash system excess pressure monitoring User input display LCD indicating system parameters Standby...

Page 202: ...ons 16 x 42 x 51 cm h x w x d PC connection All functions controllable via USB USB 1 1 or 2 0 Integrated USB hub with three USB ports USB 1 1 or 2 0 I O Interfaces 2 digital inputs 2 relay outputs 15 pin D Sub port for connection of a Solvent Rack or degasser Safety features Leak sensor active rear seal wash system excess pressure monitoring User input display LCD indicating system parameters Stan...

Page 203: ...three USB ports USB 1 1 or 2 0 I O Interfaces 2 digital inputs 2 relay outputs 15 pin D Sub port for connection of a Solvent Rack or degasser Safety features Leak sensor active rear seal wash system excess pressure monitoring User input display LCD indicating system parameters Standby button 3 LEDs Power Connected and Status for status monitoring 4 function keys for operation during initial instal...

Page 204: ...UltiMate 3000 Series SD RS and BM Pumps Page 190 Operating Instructions ...

Page 205: ...er including Filter holder top and bottom parts and filter frit stainless steel porosity 10 µm Included in Included in 6268 0115 6268 0110 1 Liquid reservoir for rear seal wash system 250 mL Included in 2270 0026 1 Bottle cap for the liquid reservoir of the rear seal wash system Included in 2270 0026 1 Retaining guide for seal wash reservoir Included in 6000 0042 2 Labels for solvent tubing 1 6 mm...

Page 206: ...tion from the pump to the WPS 3000RS 6040 2355 1 Capillary 1 16 0 25 mm I D with appropriate fittings e g to be used as spare part or for special use 6020 9110 1 Set of fitting plugs for RS SD pumps with 1 FS 8 fitting and 1 PEEK fitting extra long 6040 9101 1 Dionex menu pen 6300 0100 1 Fuse 2A slow blow 5 x 20 mm Included in 6030 9003 2 Silicone tubing O D x I D 2 80 x 1 30 mm Included in 6000 0...

Page 207: ...wrench size 6 mm 1 piston seal removal and insertion tool 1 pump head tool for RS SD pumps and 1 pump head tool for BM pumps and NCS 3500RS NCP 3200RS the tool is required for establishing the distance during piston installation 6007 9304 1 Components for UltiMate 3000 system drainage Cable clips self adhesive Y piece T piece Elbow Connecting tube Drain tubing Installation instructions Included in...

Page 208: ...r seal wash system Included in 2270 0026 1 Retaining guide for seal wash reservoir Included in 6000 0042 2 Labels for solvent tubing 1 6 mm both red and green 3 0 mm both red and green 8 6 Tool kit for UltiMate 3000 RS SD BM pumps and NCS 3500RS NCP 3200RS including 1 open end wrench 1 4 x 5 16 2 open end wrenches 11 x 13 mm 1 Allen wrench size 6 mm 1 piston seal removal and insertion tool 1 pump ...

Page 209: ... ISO 3100BM Solvent filter including Filter holder top and bottom parts and filter frit PEEK porosity 0 5 µm Included in Included in 6268 0115 6268 0118 1 Solvent line from analytical degasser to pump head 6030 2546 1 PEEK filter frit porosity 0 5 µm Included in 6268 0118 1 Liquid reservoir for rear seal wash system 250 mL Included in 2270 0026 1 Bottle cap for the liquid reservoir of the rear sea...

Page 210: ...g FS 8 PEEK with locking ring 6000 0144 1 Dionex menu pen 6300 0100 1 Fuse 2A slow blow 5 x 20 mm Included in 6030 9003 2 Silicone tubing 2 80 mm O D x 1 30 mm I D Included in 6000 0010 3 m Set of PEEK fittings for tubing O D 3 0 x I D 1 5 mm including 5 fittings 1 8 PEEK 5 locking rings PEEK 5 support flanges PEEK 2m solvent line analytical 6025 2511 1 Plastic syringe 12 mL Included in 6000 0010 ...

Page 211: ...ple injector valve suitable for pressures 50 MPa Manual sample injector valve The kit includes the valve all components required for the installation a capillary Viper SST 0 13 mm x 350 mm I D x L to connect the valve to the pump and detailed installation instructions 6040 0610 Mounting angle for sample injector valve The kit includes all components required for valve installation a capillary Vipe...

Page 212: ...mn inlet 6040 2355 Capillary 0 18 x 450 mm I D x L SST Viper e g for the connection from the SD pump to the WPS 3000SL 6040 2365 Capillary 0 18 x 350 mm I D x L SST Viper e g for the connection from the WPS 3000SL to the TCC 3000SD column inlet 6040 2375 Capillary 0 18 x 250 mm I D x L SST Viper e g for the connection from the TCC 3000SD column outlet to the DAD 3000SD MWD 3000SD or VWD 3100 6040 ...

Page 213: ...3600SD RS pumps including fittings including 2 capillaries from working cylinder to equilibration cylinder U tubes 2 capillaries from pump head to purge unit 6040 3001 Capillaries set of capillaries for LPG 3400BM and DGP 3600BM pumps including fittings including 2 capillaries from working cylinder to equilibration cylinder U tubes 2 capillaries from pump head to purge unit 2 capillaries from purg...

Page 214: ...t no 6042 3001 6040 3024 6042 3024 Capillary mixer Mixers capillary mixers Check valve cartridge identical for inlet valve nut and outlet valve nut for SD and BM pumps sapphire RS pumps ceramics Tip For SD and BM pumps the ceramics cartridge is available as an option When using acetonitrile valve problems may be observed indicated for example by increased pulsation In these cases consider replacin...

Page 215: ...xer 25 µL part no 6040 3020 in the single step mixing system Inline filter 10 µl for LPG 3400BM and DGP 3600BM 6040 5110 6040 5010 6042 5014 Inline filter filter frits Filter frits inline filter Maintenance kit for DGP 3600BM including 6 solvent filters with filter frits 1 5 m silicone tubing O D x I D 2 80 mm x 1 30 mm 18 cm Pharmed tubing O D x I D 3 2 mm x 1 6 mm 5 tubing connectors straight fo...

Page 216: ...x1 5 for seal wash system 2 valve cartridges 1 cap seal for purge valve knob only for knobs without integrated cap seal 10 cleaning swaps 6040 1956 Maintenance kit for HPG 3x00SD including 4 solvent filters with filter frits 1 5 m silicone tubing O D x I D 2 80 mm x 1 30 mm 18 cm Pharmed tubing O D x I D 3 2 mm x 1 6 mm 5 tubing connectors straight for I D 1 0 2 0 mm 8 piston seals reversed phase ...

Page 217: ...m 1 filter frit titanium 2 µm for inline filter 1 valve cartridge 1 cap seal for purge valve knob only for knobs without integrated cap seal 5 cleaning swabs 6042 1951 Maintenance kit for LPG 3400RS including 4 solvent filters with filter frits 1 5 m silicone tubing O D x I D 2 80 mm x 1 30 mm 18 cm Pharmed tubing O D x I D 3 2 mm x 1 6 mm 3 tubing connectors straight for I D 1 0 2 0 mm 4 piston s...

Page 218: ...mixer is installed when the pump is shipped and thus it is not included in the shipment 6040 5100 6040 5110 6040 5310 6040 5750 6040 5450 Mixing system volume 35 µL single step RS SD pumps except for ISO 3100SD with capillary mixer volume 25 µL and inline filter volume 10 µL 6040 5000 Normal Phase NP Kit for SD pumps including Pharmed tubing 1 m O D x I D 3 20 x 1 60 mm 1 detector NP for rear seal...

Page 219: ...cluding 5 caps to close the holes in the solvent bottle caps and 10 retaining guides for solvent bottle 6030 9101 Signal cable 6 pin mini DIN 6000 1004 Solvent line ISO 3100BM for connecting the solvent reservoir directly to the pump 6030 2548 Solvent line O D x I D 1 60 x 0 75 mm from degasser to solvent selector HPG 3400 Set of 4 solvent lines fitting connections and line labels 6035 2532 Solven...

Page 220: ... and 1 pump head tool for BM pumps and NCS 3500RS NCP 3200RS the tool is required for establishing the distance during piston installation 6007 9304 Tubing from degasser to proportioning valve LPG 3400 set of 4 tubes with fitting connections 6040 2540 Tubing from proportioning valve to pump head for LPG 3400 and DGP 3600 both SD and RS LPG 3400BM and DGP 3600BM set of 2 tubes with fitting connecti...

Page 221: ...g procedures For information about the chemical resistance of PEEK see the table Medium Concentration Temperature Maximum Duration Days Resistance yes no Acetaldehyde techn pure 23 Acetic acid 96 23 7 Acetone 100 23 7 Ammonia 28 23 7 Ammonium sulphate 23 Amyl acetate 100 23 Amyl alcohol techn pure 23 Benzaldehyde 23 7 Benzene 100 23 7 Benzene Benzene mixture 60 42 Benzoic acid 23 Borax 60 Bromine ...

Page 222: ...loric acid 37 23 Hydrofluoric acid 23 Hydrogen peroxide 30 23 7 Lactic acid 23 Magnesium chloride 23 Methanol 100 23 Methyl ethyl ketone 100 23 Methyl isobutyl ketone 100 23 Nitric acid 40 23 7 Nitric acid 65 23 7 Nitrobenzene 100 23 Paraffin oil 60 Paraffin oil 23 Perchlorethylene 100 23 Phenol diluted 23 Phenol Concentrated 23 Potassium dichromate 23 Potassium hydroxide 23 Potassium nitrate 23 P...

Page 223: ...ature Maximum Duration Days Resistance yes no Sodium thiosulphate 23 Sulphur dioxide 23 Sulphur dioxide 23 Sulphuric acid 40 130 Sulphuric acid 50 23 7 Sulphuric acid dissolved 98 23 Toluene 100 23 7 Trichlormethane chloroform 100 23 Trichloroethylene 100 23 7 Water 23 Xylene 100 23 Zinc chloride 23 ...

Page 224: ... conditions non miscible liquids may mix or miscible liquids may separate Name Acetone miscible Acetonitrile Benzene non miscible Butanol t Butylmethylether Cyclohexane Cyclopentane Dichloroethane Dichlorromethane Di Ethylether Dimethylformamide Dimethylsulfoxide Dioxan Di Propylether Acetic acid Ethanol Ethyl acetate Heptane Hexane Methanol Methyl ethyl ketone Octane Pentane Propylalcohol Tetrach...

Page 225: ...0 33 0 50 2 9 0 76 0 62 1 00 Compressibility at 20 C Mbar 1 99 97 160 100 123 93 46 Critical Flow Fc mL min 1 13 9 4 14 83 21 18 28 Linear Drop in Pressure Δp l MPa m 2 0 06 0 08 0 06 0 40 0 10 0 10 0 17 Polarity P 3 5 8 3 1 0 1 3 9 5 1 4 0 10 2 1 FC critical flow for 0 25 mm I D tubing FC mL min 113 x 0 25 mm x cP g mL Fc is an example of a hydrodynamic calculation 2 Δp l linear drop in pressure ...

Page 226: ...uid 1 Substances whose flash point is below 38 C are classified as flammable 2 The auto ignition temperature is the lowest temperature at which substances can self ignite at atmospheric pressure without an external ignition source that is without external ignition by sparks or flames The thermal energy required to reach the auto ignition temperature is created by a spontaneous chemical reaction or...

Page 227: ...Physics 79th Edition CRC Press 1998 1999 5 G W C Kaye and T H Laby Tables of Physical and Chemical Constants 16th Edition Longman Ed 1995 6 Union des Industries Chimiques L Electricité Statique en Atmosphère Explosive Septembre 1982 7 B P Mullins Spontaneous Ignition of Liquid Fuels Butterworths Ed 1955 8 Chemical Safety Sheets Working Safely with Hazardous Chemicals Kluwer Acad Publ Samson Chem P...

Page 228: ...UltiMate 3000 Series SD RS and BM Pumps Page 214 Operating Instructions ...

Page 229: ...r and label of the cable wire connected to each pin Tip If you want to connect an ESA Corona or Coulochem III detector to the pump refer to page 43 for details Pin Color Signal Name Signal Level Remarks 1 Pink Not used 2 Grey Not used 3 Green GND Ground Reference potential 4 Yellow Input 1 TTL Digital input 1 port 1 Input 2 Digital input 2 port 2 5 Brown Relay 1 out Potential free Middle contact p...

Page 230: ...with Solvent Rack leaks 5 Reserved 6 V_Degas 15V_supply Supply for the solvent rack 7 GND_Degas Ground_supply Reference potential for V_Degas 8 VCC Voltage for logic devices 9 Jumper to pin 3 10 GND Reference potential for VCC 11 GND Reference potential for VCC 12 GND Reference potential for VCC 13 Reserved 14 V_Degas 15V_supply Supply for the solvent rack 15 GND_Degas Ground_supply Reference pote...

Page 231: ...n Dialog Bottles 40 Devices 36 General 35 Inputs 41 Left Limits 39 Left Solvents 40 Limits 39 Relays 41 Right Limits 39 Right Solvents 40 Signals 41 Solvents 40 Cleaning Pistons 141 Clear 74 Commands Dialog Box 65 Commands Display 70 Compression 76 Compression Values 121 Configuration Menu 76 Configurations 12 Connection Chromeleon Computer 30 Digital I O 31 215 Fluid 20 Power Cord 31 Solvent Rack...

Page 232: ...73 Set Flow 71 Toggle 73 Fuse Cartridge 18 Fuse Replacement 158 G General Dialog Box 35 Gradient Combinations HPG 3400 181 Gradient Curves 94 Gradient Profile Specify 94 H Hold 63 HPG 3200 176 Flow Schematic 177 Interior Components 176 Operating Principle 178 HPG 3400 179 Flow Schematic 180 Gradient Combinations 181 Interior Components 179 Operating Principle 181 Solvent Selector 181 I Injection S...

Page 233: ...85 Mixing System 22 146 183 Optional 183 Single step 185 Two step 22 Model 76 Module Address 36 N Next 74 O Off 62 OK 73 Operating Instructions 1 Operating Principle 15 DGP 3600 175 HPG 3200 178 HPG 3400 181 ISO 3100BM 167 ISO 3100SD 164 LPG 3400 171 Operating Problems 114 Operation 61 Chromeleon 64 Control Elements 16 Function Keys 70 ISO 3100BM 90 Menu 72 Power Up 61 Pump Display 16 70 Safety Pr...

Page 234: ...imits Dialog Box 39 Right Solvents Dialog Box 40 RMA Number 123 Running 62 S Safety 2 Safety Precautions 3 Seal Wash System 21 83 Clean Detector 127 Connect 51 Function 84 Leakage Test 125 Peristaltic Tubing 126 Replace Detector 127 Service 125 Wash Liquid 84 Selected Pump 75 Self Test 76 Serial Number 76 Server Configuration Program 34 Server Monitor 34 Service 123 Capillary Mixer 146 Check Valve...

Page 235: ...nel 16 103 Pump Block 103 104 Status Screen 62 Stopped 63 Support Ring 142 Symbols 2 Synchronization Inject 80 T Technical Information 187 Toggle 73 Troubleshooting 103 Compression Values 121 Diagnostics Messages 112 Display Messages 105 Operating Problems 114 U Unit Description Biocompatible Pumps 14 Operating Principle 15 Overview 11 Pump Configurations 12 Unpacking 27 USB 30 USB Configuration F...

Page 236: ...UltiMate 3000 Series SD RS and BM Pumps Page 222 Operating Instructions ...

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