Dionex UltiMate 3000 Series, Operating Instructions Manual

Page: 91 / 236

UltiMate 3000 Series:
SD, RS, and BM Pumps
Operating Instructions Page 77
5.5 Information for Operating the Pump
This section provides specific information about settings and functions that should be
considered for operating the pump.
To learn more about ... On page …
Choosing the solvents See further down
Linking the pump to the autosampler 80
Setting the flow rate, flow acceleration, and flow deceleration 81
Setting the pressure limits 82
Recording the pump pressure 83
Rear seal wash system 83
Purging the Pump 85
Detecting liquid leaks in the pump 86
Adjusting the Screen Brightness or Contrast 87
SmartStartup and SmartShutdown 87
Notes on degasser operation (LPG-3400 and SRD-3x00) 88
Precautions for pump operation (ISO-3100BM) 90
Also observe the information about special functions that Chromeleon supports for operating
the pump (

page 91).
5.5.1 Choosing the Solvents
Observe the following precautions:

The pump is primed with 2-propanol. During initial operation of the pump, make sure that
the solvents used are miscible with 2-propanol. Otherwise, follow the appropriate
intermediate steps.

Use only water of at least HPLC grade or better LC-MS grade (0.2 µm, filtered).
If water from water purification systems is used that are not properly maintained,
polymeric contamination may seriously damage the column, rapidly block the solvent
frits, and result in early piston seal wear.

Use only standard solvents and buffers that are compatible with all parts of the
UltiMate 3000 system that may be exposed to solvents.
For information about the wetted parts in the pump, see the Technical Information section
(

page 187). For information about the wetted parts in the other UltiMate 3000 system
modules, refer to the 'Technical Information' section in the operating instructions for the
modules.