LC/MS and the Cone Wash
____________________________________________________________________________ Introduction
Introduction
Historically, LC/MS has been compatible only with volatile buffer systems
using modifiers such as trifluoroacetic acid, formic acid, and acetic acid.
Phosphate buffers, although extensively used in LC separations, were not
suited to LC/MS due to the rapid blocking of the ion sampling region caused
by the deposition of involatile phosphate salts. The self-cleaning API source
allows routine LC/MS with chromatographic buffers such as phosphates or
ion-pairing agents and samples in dirty matrices.
This chapter contains the following information:
Details of HPLC solvents and mobile phase additives that focus on
LC/MS applications using the MSQ.
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Instructions on how to set up the cone wash and information on when to
use it.
Instructions on flow splitting for use with hyphenated detection
applications.
____________________________MSQ Hardware Manual ____________________________
3-1
