IonPac AS9-SC Column Care
Document No. 034656C06
Page 1 of 4
RECOMMENDED OPERATION PRESSURES
Operating a column above its recommended pressure limit can cause irreversible loss of column performance. The maximum
recommended operating pressure for IonPac AS9-SC columns is 4,000 psi.
COLUMN START-UP
The column is shipped in 2.0 mM Na
2
CO
3
/0.75 mM NaHCO
3
(Eluent) storage solution.
Prepare the eluent shown on the test chromatogram, install the column in the chromatography module and test the column
performance under the conditions described in the test chromatogram. Continue making injections of the test standard until
consecutive injections of the standard give reproducible retention times. Equilibration is complete when consecutive
injections of the standard give reproducible retention times.
COLUMN STORAGE
For short-term storage (< 1 week), use Eluent, for long-term storage (> 1 week), use 100 mM Sodium Bicarbonate as the
storage solution. Flush the column with storage solution for a minimum of 10 minutes. Cap both ends securely, using the
plugs supplied with the column.
COLUMN CLEANUP
The following column cleanup protocols have been divided into three general isocratic protocols to remove acid-soluble,
basesoluble or organic contaminants. They can be combined into one gradient protocol if desired but the following
precautions should be observed.
Always ensure that the cleanup protocol used does not switch between eluents quickly, creating high pressure eluent interface
bands in the column. High pressure bands can disrupt the uniformity of the packing of the column bed and irreversibly
damage the performance of the column. High pressure bands i.n the column can be created by pumping successive eluents
that are not miscible through the column, that have eluent components in one eluent that will precipitate out when added to
the second eluent or by using an acidic eluent followed by a basic eluent with may create a neutralization pressure band. The
precipitation of the salts in solvents during column rinses can result in very high pressure bands. High viscosity mixing bands
can be created between two eluents having solvents with a very high energy of mixing.
