IonPacAS9-SC
034656-06
Page 10 of 28
SECTION 4 - OPERATIONS
4.1
General Operating Conditions
Sample Volume:
10 µL Loop + 0.8 µL Injection valve dead volume
Column:
AS9-SC 4-mm Analytical AG9-SC 4-mm Guard Column
Eluent:
1.8 mM Na
2
CO
3
/1.7 mM NaHCO
3
Eluent Flow Rate:
1.0 mL/min
SRS Suppressor:
Anion Self-Regenerating Suppressor, ASRS-ULTRA (4-mm)
AutoSuppression Recycle Mode
or MMS Suppressor:
Anion MicroMembrane Suppressor, AMMS III (4-mm)
MMS Regenerant:
50 mN H
2
SO
4
or AES Suppressor:
Anion Atlas Electrolytic Suppressor, AAES
Expected Background Conductivity:
15 - 20 µS
Long-term Storage Solution (> 1 week):
100 mM Sodium Bicarbonate
Short-term Storage Solution (< 1 week):
Eluent
The selectivity of the IonPac AS9-SC 4-mm Analytical Column has been designed to separate F
-
, ClO
2
-
, BrO
3
-
, Cl
-
, NO
2
-
, Br
-
, ClO
3
,
NO
3
-
, HPO
4
2-
and SO
4
2-
isocratically in less than 10 minutes. The AS9-SC packing is a highly cross-linked (55%), microporous
resin that has been agglomerated with totally permeable latex particles that are completely aminated. The latex particles carry the
actual ion exchange function - an alkanol quaternary ammonium group. The polyacrylic structure of the latex MicroBeads make
the AS9-SC compatible with pH 2-11 eluents. The highly cross-linked substrate core renders the AS9-SC compatible with eluents
containing 0-100% HPLC solvents organic solvents. The AS9-SC can be used with any suppressible ionic eluent that does not
exceed the capacity of the suppressor.
4.2
IonPac AS9-SC Operation Precautions
CAUTION
Filter and Degas Eluents
Filter Samples
Eluent pH between 2 and 11 and contains no hydroxide
Sample pH between 2 and 13
3 mL/min Maximum Flow Rate
4.3
Chemical Purity Requirements
Obtaining reliable, consistent and accurate results requires eluents that are free of ionic impurities. Chemicals, solvents and
deionized water used to prepare eluents must be of the highest purity available. Low trace impurities and low particle levels in
eluents also help to protect your ion exchange columns and system components. DIONEX cannot guarantee proper column
performance when the quality of the chemicals, solvents and water used to prepare eluents has been compromised.
4.3.1 Inorganic Chemicals
Reagent Grade inorganic chemicals should always be used to prepare ionic eluents. Whenever possible, inorganic chemicals that
meet or surpass the latest American Chemical Society standard for purity should be used. These inorganic chemicals will detail
the purity by having an actual lot analysis on each label.
4.3.2 Solvents
Since solvents used with the IonPac AS9-SC columns are added to ionic eluents to modify the ion exchange process or improve
sample solubility, the solvents used must be free of ionic impurities. However, since most manufacturers of solvents do not test
for ionic impurities, it is important that the highest grade of solvents available be used. Currently, several manufacturers are
making ultrahigh purity solvents that are compatible for HPLC and spectrophotometric applications. These ultrahigh purity
