IonPacAS9-SC
034656-06
Page 18 of 28
5.3
Resolution of Low-Concentration Analytes - EPA Water Matrix
The following example demonstrates the separation of sample analytes having highly variable concentrations. Setting the flow
rate at 1.0 mL/min allows for increased resolution of very low concentration analytes such as bromate, chlorite, and chlorate in
the following EPA water matrix sample.
Sample Loop Volume:
100 µL
Analytical Column:
IonPac AS9-SC Analytical Column
Eluent:
1.8 mM Na
2
CO
3
/1.7 mM NaHCO
3
Eluent Flow Rate:
1.0 mL/min
SRS Suppressor:
Anion Self-Regenerating Suppressor, ASRS-ULTRA (4-mm)
AutoSuppression Recycle Mode
or AES Suppressor:
Anion Atlas Electrolytic Suppressor, AAES
or MMS Suppressor:
Anion MicroMembrane Suppressor, AMMS III (4-mm)
MMS Regenerant:
25-50 mN H
2
SO
4
Expected Background Conductivity:
12-16 µS
Long-term Storage Solution (> 1 week):
100 mM Sodium Bicarbonate
Short-term Storage Solution (< 1 week):
Eluent
1
2
3
4
5
6
7
8
9
10
2
1
0
-1
µS
0
5
10
15
20
Peaks
mg/L
1.
Fluoride
5.66
2.
Chlorite
0.50
3.
Bromate
0.20
4.
Chloride
45.0
5.
Nitrite
0.06
6.
Bromide
0.08
7.
Chlorate
0.14
8.
Nitrate
42.0
9.
Phosphate
0.17
10. Sulfate
50.0
Figure 3
EPA Water Matrix
