IonPacAS9-SC
034656-06
Page 13 of 28
4.6
Eluents that Contain Solvents
When mixing solvents with water remember to mix solvent with water on a volume to volume basis. For example, if a procedure
requires an eluent of 90% acetonitrile, prepare the eluent by adding 900 mL of acetonitrile to an eluent reservoir. Then add 100
mL of deionized water to the acetonitrile in the reservoir. Using this procedure to mix solvents with water will ensure that a
consistent true volume/volume eluent is obtained. Premixing water with solvent will minimize the possibility of outgassing.
NOTE
When degassing eluents containing solvents, do not degas the eluent excessively since it is possible that a volatile
solvent can be “boiled” off from the solution.
The AS9-SC can withstand common HPLC solvents in a concentration range of 0 - 100%. Solvents and water should be premixed
in concentrations to allow proper mixing by the gradient pump and to minimize outgassing. Ensure that all of the inorganic
chemicals are soluble in the highest solvent concentration to be used during the analysis so that salts do not precipitate out in either
the pump or the column.
Table 6
HPLC Solvents for Use with IonPac AS9-SC Columns
Solvent
Maximum Operating
Concentration
Acetonitrile
100%
Methanol
100%
2-Propanol
100%
Tetrahydrofuran
20%
When using a solvent in an ionic eluent, column generated backpressures will depend on the solvent used, concentration of the
solvent, the ionic strength of the eluent and the flow rate used. The column back pressure will vary as the composition of water-
methanol and water-acetonitrile mixture varies.
The practical backpressure limit for the IonPac AS9-SC columns is 4,000 psi. Therefore, any combination of the above,
contributing to operating backpressure that totals up to 4,000 psi, can be used.
4.7
Regenerant Preparation for the AMMS III
The regenerant is 50 mN sulfuric acid. Dilute 100 mL (about 100 g) of 0.50 N sulfuric acid (P/N 037164 or P/N 039601) to 1 L
using deionized water. If you are not using the AutoRegen Accessory (P/N 039594), prepare several liters of the regenerant.
For a guide to properly adjusting the regenerant flow rate, see Document No. 031727, the Product Manual for the Anion
MicroMembrane Suppressor III, the AMMS III.
