IonPac AS9-SC Column Care
Document No. 034656C06
Page 3 of 4
METAL CONTAMINANTS
WARNING
Do not use the standard HCl procedure described in other
DIONEX documentation for base cleanup of IonPac AS9-SC/AG9-SC Columns.
This procedure will destroy IonPac AS9-SC/AG9-SC Columns
A. Prepare a 500 mL cleanup solution of 100 mM oxalic acid/50 mM NaOH (pH 2.5 to 3.0) by dissolving 6.2 g of oxalic
acid dihydrate with 4.2 g (2.6 mL) of 50% w/w sodium hydroxide concentrate solution with 400 ml of deionized water
having a specific resistance of 18.2 megohm-cm. After thoroughly mixing, dilute to a final volume of 500 mL with
deionized water having a specific resistance of 18.2 megohm-cm.
B. Disconnect the anion suppressor from the IonPac AS9-SC Analytical Column. If your system is configured with both a
guard column and an analytical column, reverse the order of the guard and analytical column in the eluent flow path.
Double check that the eluent flows in the direction designated on each of the column labels. Direct the effluent from the
outlet line of the AS9-SC Guard Column to a separate waste container.
CAUTION
When cleaning an analytical column and a guard column in series, ensure that the guard column is placed after the
analytical column in the eluent flow path. Contaminants that have accumulated on the guard column can be eluted
onto the analytical column and irreversibly damage it. If in doubt, clean each column separately.
C. Set the pump flow rate to 1.0 mL/min.
D. If your eluent contains a solvent that is not compatible with the cleanup solution of 100 mM oxalic acid/50 mM NaOH,
rinse the column for 15 minutes with deionized water before pumping the cleanup solution over the column.
E. Pump the cleanup solution through the column for 30-60 minutes
F. If the application eluent contains a solvent that is not compatible with the cleanup solution, rinse the column for 15
minutes with deionized water. Then pump the application eluent over the column.
G. Reconnect the AMMS III to the AS9-SC Analytical Column and place the guard column in line between the injection
valve and the analytical column if your system was originally configured with a guard column.
H. Equilibrate the column(s) with the application eluent before resuming normal operation.
