x.sight - software manual
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5.6 Set Region of Interest (ROI)
Once the cartridge is loaded into the x.sight device and the device lid is closed, you have
to set the ROI. You can either click on the [
Autodetect ROI
] button or click directly into the
[
Cell Camera
] window. Refer to chapter 3.6 for more details.
Make sure that the ROI is centered over the nozzle. The result is displayed in the [
Cell
Camera
] window as three circles (blue-green-blue).
5.7 Performing Droplet-QC
Before starting an experiment with a new loaded cartridge, you must perform droplet
quality control (DQC). To enter DQC mode press the [
DROPLET-QC
] button on the main
menu:
The system will move the dispenser to the QC position and the DQC screen appears.
Use the [
Droplet Position
] sliders to navigate the droplet into the center of the live image
window. The droplet should appear at a range of 2-5 ms in the [
Vertical/Delay
] slider which
you should run first. Then tune the [
Horizontal
] and finally the [
Focus
] slider until the droplet
appears like shown:
