x.sight - software manual
24 |
P a g e
4
f.sight special
The following section covers functions and features that are only available in f.sight
models.
4.1 Cell camera modes
f.sight models carry two independent cameras, one for bright field and one for fluorescence
detection. Below the
Cell Camera
section there are several f.sight specific functions to
control the different cameras.
The [
Overlay
] button allows for displaying the detected cell contours as an overlay on top
of the camera window. All detected objects will then be surrounded by the respectively
detected contour. The following image shows the difference when Overlay is off (left
picture) and on (right picture). The blue circles represent the ROI, the green contour
represents the cell.
Hint: Using the overlay function will increase the required computing time and
thus slow down the process. Especially, when a sample with higher
concentration is loaded. The overlay may be helpful during setup to verify if your
settings are correct. It is recommended to switch off the function during standard
printing runs to speed up the process.
An additional drop-down menu is displayed below the cell camera window. It allows for
switching between the bright field and fluorescence camera channels and it provides the
possibility to show a combined two channel image. Options here are:
