x.sight - software manual
19 |
P a g e
The live video stream displays the ROI (
so called “region of interest”) as three circles
around the nozzle. The green circle of the ROI defines the fluid volume that is expelled
from the chip nozzle when a droplet is generated. The two blue circles define three areas
(A-C) like shown the schematic below:
Only when a single-cell is detected inside region
A
(inner blue circle) and no other object
is inside region
B
(between the two blue circles), the single-cell is printed to the substrate.
The correct position of the ROI is crucial to achieve good printing results. The following
reference shows you how to set the ROI. The ROI is vertically centered in the nozzle
(vertical yellow dashed line, left figure). Horizontally, the inner blue circle should touch the
nozzle orifice (red red dashed line, right figure).
