x.sight - software manual
11 |
P a g e
Some cell media tend to evaporate quickly on the open nozzle. This leads to
crystallization of the nozzle. The nozzle then is clogged after some minutes. The
continuous, slow generation of droplets prevents this.
The section [
Tracking Algorithm
] contains the two main properties for the cell detection
algorithm.
The [
Detection Radius
] defines the ROI radius in micrometers. Default value is 125. It can
be adapted for specific needs (e.g. very low or high viscous media).
The [
Detection Threshold
] defines the threshold between foreground (= cells or particles)
and background (= chip) of the image. The threshold required to distinguish foreground
objects from the background in image processing. The lower the contrast of objects
against the background, the lower the threshold has to be set. Default value is 25. The
value is automatically taken over and is set permanently until changed, even when
software is restarted.
Hint: The cell detection is sensitive against this value. Too low values will
generate noise and ghost objects that disturb detection. Too high values will
prevent detection of any object, even those with high contrast. Find a suitable
value for your application. A good range is 20 - 40.
The section [
Deionisator
] allows you to enable automatic plate deionization prior to
printing. To enable it, turn on the [
Perform Deionisation
] switch. A white switch represents
“off” and a colored switch represents “on” state.
When being activated, each plate is deionized before the actual printing experiment is
performed. The deionization process takes only seconds.
Hint: When using foil based plates (e.g. PCR plates) deionization is
recommended to activate. Droplets may carry electric charge. When the plate is
ionized as well, droplets may not land in the wells precisely.
When using “Offset Compensation” mode, deionization must be activated to
ensure straight droplet flight into the plate.
