x.sight - software manual
16 |
P a g e
The interactive well plate [
MTP Configuration
] allows for
•
selection of single wells by left mouse click
•
selection of multiple wells by CTRL + left mouse click
•
selection of multiple wells by a rubberband (drag, hold, release left mouse)
•
selection of all wells by left mouse click into the grey top left corner of the plate
When at least one well is selected, below the plate the configuration for the selected well(s)
appears. This includes:
•
[
Action
]
defines the action to be performed in the well(s)
•
[
No. of …
]
defines the number of events to be performed
•
[
Size
]
defines the range of cell diameter for cells to be sorted
•
[
Roundness
] defines the range of cell roundness for cells to be sorted
•
[
FL intensity
] defines the range of fluorescence intensity
As [
Action
] you can choose from
•
[
Print cell
]
prints a single droplet that contains one cell
•
[
Print FL cell
]
prints a single droplet that contains one fluorescent cell
1
•
[
Print NTC
]
prints a void single droplet (no cell inside)
•
[
Print every droplet
] prints a single droplet independent from its content
Hint: Besides printing single-cells, the software is also capable of delivering
empty droplets as a negative template control (NTC) for applications that use
assays after cell sorting.
Also the software can print droplets independent from their content (void, single-
cell or multiple cells). This function enables quick delivery of a certain sample
volume into specific wells.
The [
No. of …
] input field allows for defining the repetitions of the chosen [
Action
] to be
performed in the specific well(s). It changes depending on the action.
Example: the following example illustrate how Action and No. of are related to
each other and how you can generate different configurations.
[Action] = [Print cell] and [No. of cells] = 10
→
prints 10 cells into the well
[Action] = [Print every droplet] and [No. of droplets] = 100
→
prints 100 droplets into the well, no matter what they contain
[Action] = [Print NTC] and [No. of NTC] = 1
→
prints 1 void droplet into the well
1
Available for f.sight only.
