x.sight - software manual
12 |
P a g e
The section [
Offset Compensation
] offers a high precision printing mode. To enable this
mode, turn on the [
Use Offset Correction
] switch. A white switch represents “off” and a
colored
switch represents “on” state.
The [
Offset Compensation
] measures the droplets flight trajectory in space and corrects
the axis system with an offset to ensure that the droplet is landing centered in the well.
The automatic correction requires the distance the droplets travels until reaching the
bottom of the well. The input field [
MTP Well Depth
] let you enter this distance in
millimeters.
When being activated, this mode performs the compensation prior to each experiment run.
Compensation can take up to 3 minutes. It is fully automatic.
Hint: The [Offset Compensation] mode is recommended when using targets
that require a high precision in droplet positioning. For example 384-well plates
with conical wells for PCR or sequencing applications.
It can also be useful when targeting special plates or special applications where
the position of the single-cell on the target is crucial.
Using this mode may significantly increase the total process time per plate.
The section [
Automation Settings
] contains the relevant settings for running the x.sight in
an automation environment using the cytena API.
To connect the software with the client, the opposite server has to be started as well. Next
to [
Automation Server
] use the [
Start
] button to connect server and client. [Server Port
Number] refers to the TPC/IP Port used for connection. [
Dispensing Event Warning
Threshold
] and [
Dispensing Event Error Threshold
] refer to the warning and error events
being sent to the automation controller. A counter detects the number of dispenses without
a single-cell event. If the respective thresholds are exceeded, the warning or error is
transmitted.
Hint: For further information please refer to the API handbook.
