background image

Instruction Manual   |   13

 10.  Troubleshooting

If you notice an improper pipettor operation, identify the 

cause and eliminate the fault. Follow the instructions in 

the sequence provided. Replacement of parts should be 

required only occasionally and should not occur under 

normal pipettor use.

Problem

Cause

Solution

Droplets of 

liquid remain 

in the pipet 

tip.

The pipet tip is 

emptied too fast.

Decrease the speed 

of pressing the pipettor 

pushbutton.

Press the pushbutton to the 

second stop to expel all the 

droplets.

The pipet tip 

wettability has 

increased due to 

extensive use.

Replace the tip with 

a new one.

Droplets 

of air appear 

in the liquid 

aspirated 

into the pipet 

tip.

The pipet tip 

immersion 

is too shallow.

Immerse the tip 

deeper according to the 

instructions.

The pipet tip is 

incorrectly pressed 

onto the pipettor 

shaft.

Press the pipettor firmly.

The tip is damaged 

or worn out due to 

extensive use.

Replace the tip with 

a new one.

The pipettor 

incorrectly 

aspirates 

the liquid 

or the liquid 

drops out 

from the tip.

The pipet tip is 

incorrectly pressed 

onto the pipettor 

shaft.

Press the pipet tip firmly.

The pipettor 

incorrectly 

aspirates 

the liquid 

or the liquid 

drops out 

from the tip.

The shaft nut is 

loose in the single-

channel pipettors 

2 μL to 1,000 μL.

Tighten the shaft nut.

The sealing surface 

of the shaft is 

cracked or scored.

Remove the tip ejector. Unscrew 

the shaft nut and inspect the 

shaft and the piston assembly. 

Replace the damaged parts. 

When reassembling the 

pipettor, the nut should be 

hand-tightened. In the single-

channel pipettor model, the 

damage of the shaft may also 

cause a damage of the piston 

assembly. Replace the damaged 

parts. When reassembling the 

pipettor, the nut should be 

hand-tightened. 

Summary of Contents for Lambda Plus

Page 1: ...Corning Lambda Plus Pipettors Single channel 8 channel and 12 channel Pipettors Instruction Manual...

Page 2: ...ng the Volume 5 4 Aspiration and Dispensing Instructions 6 5 Pre rinsing 8 6 Dense and Viscous Liquids 8 7 Corning Pipet Tips 9 8 Recommendations 9 9 Recalibration 10 10 Troubleshooting 13 11 Cleaning...

Page 3: ...ettors are produced in four volume ranges 0 5 to 10 L 5 to 50 L 20 to 200 L 50 to 300 L Model Volume Range L Application 2 10 Measurement and transfer of micro volumes DNA sequencing and enzyme assay...

Page 4: ...jector D Single channel Pipettor Ejector The ejector length can be adjusted to accommodate all common brands of pipet tips When using narrow tubes it may be necessary to remove the tip ejector This is...

Page 5: ...ng at least 10 measurements of distilled water at a reference temperature of 20 C 1 C according to EN ISO 8655 standard 8 channel and 12 channel Pipettor Ejector The 8 and 12 channel pipettor construc...

Page 6: ...the test volume selected Precision is the degree to which repeated measurements under unchanged conditions show the same results The accuracy and precision are obtained gravimetrically using Corning...

Page 7: ...nd red Red numbers represent decimal fractions for microvolume pipettors or milliliters for macrovolume pipettors An example for each of these pipettors is given on the next page Nominal Volume L Coun...

Page 8: ...too far below the required value the process must be repeated Set the locking ring into the upper position following the volume adjustment thus locking the knob and preventing accidental volume chang...

Page 9: ...to the recommended depth or if the pipetting pushbutton is rapidly released air may enter the disposable pipet tip NOTE Avoid touching the orifice of the pipet tip Dispensing 3 Place the end of the pi...

Page 10: ...sion are based on pipetting distilled water Handling of liquids with physical qualities of density viscosity and surface tension which differ substantially from water may need to be gravimetrically ch...

Page 11: ...ry and should remain constant during aspiration The pipettor should be held in a vertical position Change the pipet tip when volume setting is changed or when a different liquid is to be aspirated Cha...

Page 12: ...ical Density of the liquid used is close to that of distilled water Balance with appropriate sensitivity has been used Conversion factor mg L has been taken into account The requirements shown in Sect...

Page 13: ...0 33 8 50 12 50 5 50 5 4 8 5 2 1 67 8 200 12 200 20 200 20 19 4 20 6 6 30 8 300 12 300 50 300 50 49 2 50 8 10 00 Perform 5 aspirations weigh each one and calculate the average value of the aspiration...

Page 14: ...o reduce the aspirated volume or counterclockwise to increase the volume One full turn of the calibration key changes the pipettor aspiration volume by the amount given in the table 4 Take out the cal...

Page 15: ...ns The pipet tip is incorrectly pressed onto the pipettor shaft Press the pipettor firmly The tip is damaged or worn out due to extensive use Replace the tip with a new one The pipettor incorrectly as...

Page 16: ...eps No lubricant on the sealing elements Remove the tip ejector Unscrew the shaft nut and remove the shaft piston assembly seal and O ring Rinse the removed parts in distilled water and dry thoroughly...

Page 17: ...ried and cooled to room temperature It is recommended to Unscrew the shaft nut slightly prior to sterilization and re tighten after autoclaving Set the locking ring in lower unlocked position prior to...

Page 18: ...ion key M Ejector cap N Ejector pushbutton P Identification ring S Locking ring Please contact Corning Customer Service to inquire about availability of spare parts Pipettor model and name of the part...

Page 19: ...not arising from defects in original material or workmanship This warranty does not cover the PTFE seal O ring or shaft Claims for transit damage should be filed with the transportation carrier In the...

Page 20: ...re for research use or general laboratory use only Not intended for use in diagnostic or therapeutic procedures Not for use in humans These products are not intended to mitigate the presence of microo...

Page 21: ...wan t 886 2 2716 0338 EUROPE CSEurope corning com France t 0800 916 882 Germany t 0800 101 1153 The Netherlands t 20 655 79 28 United Kingdom t 0800 376 8660 All Other European Countries t 31 0 206 59...

Reviews: