10 | CORNING LAMBDA PLUS PIPETTORS
9. Recalibration
The accuracy and precision of the pipettors are obtained
gravimetrically using Corning® pipet tips, performing at
least 10 measurements of distilled water according to
EN ISO 8655 standard.
If during pipettor operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in Section 1, the pipettor recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been met
during error determination:
◗
Ambient temperature and the temperature of the
pipettor, pipet tips, and water were identical.
◗
Density of the liquid used is close to that of distilled water.
◗
Balance with appropriate sensitivity has been used.
◗
Conversion factor mg/μL has been taken into account.
◗
The requirements shown in Sections 3 and 7 have
been met.
Volume Checked
(μL)
Balance Sensitivity
(mg)
0.1 - 10
≤0.001
10 - 100
≤0.01
>100
≤0.1
If the above conditions are satisfied and the accuracy
error for selected volume given in Section 1 exceeds the
permissible value, the pipettor recalibration procedure
should be continued.
The recalibration can be performed within one full turn of
the calibration key to the right or to the left only.
Recalibration Conditions
Ambient temperature and the temperature of the pipettor,
pipet tips, and liquid should be within the range of 20°C to
25°C and stabilized during weighing within ±0.5°C.
◗
Measurements should be conducted using distilled water.
◗
Balance sensitivity should be suitable for the volume to
be measured.
