Biotek 800 TS Series, Instructions For Use Manual

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50 | Liquid Tests
BioTek Instrument, Inc.
Example: Five readings in well A1 of 0.802, 0.802, 0.799, 0.798, and 0.801 result in a
Mean of 0.8004 and a Standard Deviation of 0.0018. The Mean multiplied by 0.5%
(0.8004 * 0.005) equals 0.004, and when added to 0.005 equals 0.009; this is the Allowed
Deviation for well A1. Since the Standard Deviation for well A1 is less than this value, the
well meets the test criteria.
3. The plate is read five times in the “Turnaround” position at 405 nm. Calculate the Mean
OD of those five reads for each well in columns 11 and 12.
4. Perform a mathematical comparison of the Mean values for each well in its Normal and
Turnaround positions (that is, compare A1 to H12, A2 to H11, B1 to G12,… H2 to A11). To
pass the test, the differences in the compared Mean values must be within the Accuracy
specification for a 96-well microplate.
Example: If the Mean value for well A1 in the Normal position is 1.902 with a specified
accuracy of ±1.0% ±0.010 OD, then the expected range for the Mean of the well in its
Turnaround (H12) position is 1.873 to 1.931 OD. 1.902 x 0.010 + 0.010 = 0.029; 1.902 -
0.029 = 1.873; 1.902 + 0.029 = 1.931.