Irys® User Guide
38
7/10/2015
For Research Use Only. Not Intended For Diagnostic Purposes.
Each Bump Time trial alternates between one of two horizontal positions on the IrysChip: either one-third of the
way across, or two-thirds across the flowcell. Before each Bump Trial, the DNA will be concentrated and spiked
into the IrysChip, without the live view from the Concentration Voltage Optimization step.
Note:
For each additional Bump Time Optimization, there is an additional one minute preparation step. Up to
four of the most recent Bump Time measurements are displayed under Recorded Bump Time(s); and Optimized
Bump Time is displayed as an average of all recorded Bump Trials in a user-editable field. It is recommended that
the user continue to measure Bump Time until two consecutive measurement are within 2 seconds of each other.
Figure 37: Bump Time Optimization
Users have the option to finish the Generate Optimized Recipe creation after Bump Time optimization; however,
optimizing the Final Load Time is recommended. If Yes, continue to Yes Pathway. If No, continue to No Pathway.
Figure 38: Option to Stop Optimization After Bump Time
