Irys® User Guide
ii
Contents
Legal Notices ................................................................................................................................................................ 4
Revision History ........................................................................................................................................................... 5
Safety Warnings ............................................................................................................................................................ 6
Chapter 1 Overview ...................................................................................................................................................... 7
1.1
Introduction ....................................................................................................................................................... 7
What's New! ................................................................................................................................................................ 7
Audience and Intended Use ........................................................................................................................................ 7
1.2
Irys Overview .................................................................................................................................................... 7
Irys Description ............................................................................................................................................................ 7
Irys Exterior ................................................................................................................................................................. 9
Front ............................................................................................................................................................................ 9
Right Side .................................................................................................................................................................... 9
Rear Panel ................................................................................................................................................................... 9
Irys Inside .................................................................................................................................................................. 10
1.3
Irys Workstation Configuration ...................................................................................................................... 11
Data Network Connections and Power ..................................................................................................................... 11
Computer System Workflow ...................................................................................................................................... 12
1.4
First Use .......................................................................................................................................................... 12
1.5
User Supplied Consumables ......................................................................................................................... 13
IrysChip ..................................................................................................................................................................... 13
IrysPrep ..................................................................................................................................................................... 13
1.6
Run Type Recipes (M2, M3, M3x and Optimized) .......................................................................................... 13
Chapter 2 Irys Instrument Control Software ............................................................................................................ 14
2.1
Best Practices ................................................................................................................................................. 14
2.2
Irys ICS Main Screen Overview ..................................................................................................................... 14
Select User Screen ................................................................................................................................................... 14
Chapter 3 Starting a Project ...................................................................................................................................... 15
3.1
Irys System Project Workflow Overview ....................................................................................................... 15
3.2
Sample Preparation Summary ...................................................................................................................... 15
IrysPrep Plug Lysis Long DNA Isolation (BNG PN 30026) ....................................................................................... 15
IrysPrep Labeling-NLRS (BNG PN 30024) ............................................................................................................... 16
IrysPrep Human Blood Protocol (BNG PN 30033).................................................................................................... 16
3.3
IrysChip Preparation Summary ..................................................................................................................... 16
Preparing IrysChip..................................................................................................................................................... 16
3.4
Instrument Preparation Summary ................................................................................................................. 16
System Startup .......................................................................................................................................................... 16
3.5
Logging In ....................................................................................................................................................... 18
Select Existing User or <Add New User> ................................................................................................................. 19
Configuring the User Settings and Remote Data Storage Location ......................................................................... 19
Chapter 4 Performing a Run ...................................................................................................................................... 20
4.1
Loading the Sample ........................................................................................................................................ 20
Load Inlets ................................................................................................................................................................. 21
Load Outlets .............................................................................................................................................................. 22
Cleaning and Oiling the Objective ............................................................................................................................. 22
Forward ..................................................................................................................................................................... 23
Sample Detect ........................................................................................................................................................... 23
4.2
Barcode............................................................................................................................................................ 24
Forward ..................................................................................................................................................................... 24
4.3
Focus & Register ............................................................................................................................................ 25
4.4
Select Recipe .................................................................................................................................................. 26
