Irys® User Guide
07/10/2015
33
For Research Use Only. Not Intended For Diagnostic Purposes.
Labels A through G
A.
Process Step
: Status indicates the state of the optimization process, which includes: Focusing, Preparing
Chip, Loading Step, Imaging Example Scan, and Assembling Mosaic.
B.
Detail Information
: In the description, users are asked to make a series of judgments and selections based on
observations in the live image screen.
C.
Proceed Step
: Action Buttons are OK, Yes, and No. These action buttons lead to the next step in the
Generate Optimized Recipe screen.
D.
Camera Live Screen
: The live image exists in two states: Camera ON (active) and Camera OFF (inactive).
In the Camera ON state, the images will be blue to reflect the laser color used for the DNA backbone, and
users will be asked to make judgments during the optimization steps. When the live image is active, users
may also use the touchscreen to move vertically and horizontally similar to the Focus and Register screen. In
the Camera OFF state, all touchscreen-based movement is disabled and the live image will indicate Camera
OFF showing the last image taken by the camera. There are some restrictions on vertical movements.
E.
Flowcell
: This displays the flowcell being used to generate an Optimized Recipe.
F.
Revert to Live
: Pressing the Home button brings live image back to the instrument calibrated home position.
Home is the starting position when the live image became active for a particular step.
G.
Time Remaining
: The timer shows the time remaining to complete a step.
H.
Navigation Buttons
: Press right arrow to proceed to next step; press left arrow to go to previous screen.
Step 1: Selecting a Base Recipe
M2, M3 and M3x are base recipes that can be optimized. M2 is the legacy recipe offered in previous versions of
Irys; and M3 is the new high-throughput recipe. M3 is the recommended base recipe to be used with IrysPrep
NLRS Experienced User Card (PN 30024); M3x modified high throughput recipe is used in certain cases. See
http://www.bionanogenomics.com/support/
.
1. Use the drop-down menu to select a base recipe and press OK to proceed.
Note:
The Generate Optimized Recipe will optimize the following parameters: Concentration Voltage,
Concentration Time, Bump Time, and Load Time.
Figure 28: Selecting a Base Recipe
