36
5061-049 REV 1.4
25-9574-75UM AE 05/2010
36
0.001 to 9999). Use C to backspace and clear the last digit entered and the up
and down arrows to move between boxes. Pressing the down arrow from the last
standard will bring up the OK box.
Press OK
to accept the calibration and go to the Results Screen (see below)
OR
Press Back
Esc
to return to the Standards Screen.
Results Screen
Step 14
Pipette on the reference sample and lower the sampling head. If Auto-Read is
off, press the 0A/100%T key. This will be used for all subsequent samples until
changed. If QA is switched on the sample will need to be replaced and the
0A/100% T key pressed again.
Step 15
Clean the top and bottom plates, pipette on the sample and lower the sampling
head. If Auto-Read is off, press
. The concentration of the sample is taken and
displayed.
Repeat step 15 for all samples.
Press
Esc
to return to the Protein Screen.
Press
˛ to display available Options which are described below.
Options (select using keypad numbers)
1. Return to Parameters Screen (step 1 above).
2. Print result via selected method.
3. Toggle Graph on/off. Displays the calibration graph, cursors give values for last
measured sample.
7. Sample Number – add a prefix to the sample number and reset the
incrementing number to the desired value.
8. Save Method – use the alpha-numeric keys to enter a name for the method and
press Save
.
9. Auto-Print – toggles Auto-Print on/off.
Exit Options by pressing
Esc
, or wait.
4.3.6. Lowry
The procedure is as follows:
Step 1
Press 5 to select Lowry method.
Step 2
The Wavelength for this method is fixed at 750 nm.
Step 3
Enter the number of Standards (1–9) to be used in the curve using the keypad
numbers or left and right arrows.
Press the down arrow.
Step 4
Units: The user can enter a text string up to 8 characters long. To access a list of
pre-defined units press the Options key
and then use the left/right arrows
to select from µg/ml, µg/µl, pmol/µl, mg/dl, mmol/l, µmol/l, g/l, mg/l, µg/l, U/l, %,