22
5061-049 REV 1.4
25-9574-75UM AE 05/2010
22
Calculated factor: this is just the calculated molecular weight divided by the
theoretical Absorbance.
The procedure is as follows:
Step 1
Press 4 to enter the Tm Calculation Parameters Screen.
Step 2
Select the Base Type: DNA or RNA.
Press the down arrow.
Step 3
Select whether the sample is Phosphorylated or not (Yes or No).
Press the down arrow.
Step 4
Enter the Primer Conc. using the keypad numbers (range 0.000 to 99.9) in
pmole/ml.
Press the down arrow.
Step 5
Enter the Buffer Molarity (buffer mo total molarity of salt in moles/l) using
the keypad numbers (range 0.000 to 10).
Press the down arrow.
Step 6
Select the Counter Ion: Na, K, TEA or Other.
Step 7 (if Other selected, see the previous section)
Enter the molecular weight (Other MW) of the Counter Ion used.
Step 8
Press Next
to select these parameters and go on to the Base Sequence
Screen
OR
Press Cancel
Esc
to return to the Life Science Screen.
Step 9
Select the Pathlength of the sample cells. Options are 0.5 mm, 0.2 mm or
Automatic.
Step 10
Enter the known Base Sequence triplets using the number keys: 1 for A, 3 for C, 4
for G and 6 for T or U. Note that a comma is added after each triplet to improve
readability
Step 11
Press OK
to select these parameters and start to measure Tm
OR
Press Cancel
Esc
to return to the Parameters Screen.
