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5061-049 REV 1.4
25-9574-75UM AE 05/2010
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Step 11
Enter the Dilution Factor (if applicable)
Step 12 (Dilution Factor known)
Enter the Dilution Factor using the keypad numbers (range 1.00 to 9999). Use the C
button to backspace and clear the last digit entered.
OR
Step 13 (calculate Dilution Factor)
Press
to enter the Dilution Factor Screen (see right). Enter the Volume of
the sample using the keypad numbers (range 0.01 to 9999). Press the down arrow.
Enter the volume of the Diluent using the keypad numbers (range 0.01 to 9999).
Press OK
to calculate the Dilution Factor and return to the Parameters Screen.
OR Press
Esc
to cancel the selections and return to the Parameters
Step 14
Select the Nucleic acid being used (or none if only the dye is being measured).
The system will select the appropriate factor. If a different factor is desired select
Custom and the Factor can be user defined.
Press OK
to enter the Results screen.
OR Press
Esc
to cancel the selections and return to the Parameters data entry
screen.
Step 15
Pipette on the reference sample and lower the sampling head. If Auto-Read is
off, press the 0A/100%T key. This will be used for all subsequent samples until
changed. If QA is switched on the reference sample will need to be replaced and
the 0A/100% T key pressed again.
Step 16
Clean the top and bottom plates, pipette on the sample and lower the sampling
head. If Auto-Read is off, press
. The system returns the dye concentration(s).
Repeat step 17 for all samples.
Press
Esc
to return to the Life Science Screen.
Press
to display available Options which are described below.
Note that very high dye concentrations may leave visible deposits on the sample
plates. This can easily be removed with a gentle rub with ethanol.
Options (select using keypad numbers)
