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5061-049 REV 1.4
25-9574-75UM AE 05/2010
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Step 12 (Standards selected)
Press Replicates
to display the replicate entry boxes. Use C to clear previously
stored results before measuring.
Clean the top and bottom plates, pipette on the replicate standard and lower the
sampling head. If Auto-Read is off, press
to measure the standard and store
the result.
Repeat for all replicates and standards. Use Next
to bring up fields for
the next standard. A graph will display the results and the fitted curve as the
measurements are input.
Use the up and down arrows to select a standard to be repeated if a poor reading
has been obtained. Use C to clear the previous reading.
Step 13 (Standards/Manual selected)
Press OK
to accept the calibration and go to the Results Screen (see below)
OR
Press Back
Esc
to return to the Standards Screen.
Calibration (Manual entry)
Shows previously entered calibration values and allows values to be entered via
the keypad.
The highlighted box can be edited in order to enter an Absorbance value
corresponding to a given concentration value using the keypad numbers (range
0.001 to 9999). Use C to backspace and clear the last digit entered and the up
and down arrows to move between boxes. Pressing the down arrow from the last
standard will bring up the OK box.
Press OK
to accept the calibration and go to the Results Screen (see below)
OR
Press Back
Esc
to return to the Standards Screen.
Results Screen
Step 14
Pipette on the reference sample and lower the sampling head. If Auto-Read is
off, press the 0A/100%T key. This will be used for all subsequent samples until
changed. If QA is switched on the sample will need to be replaced and the
0A/100% T key pressed again.
Step 15
Clean the top and bottom plates, pipette on the sample and lower the sampling
head. If Auto-Read is off, press
. The concentration of the sample is taken and
displayed.
Repeat step 15 for all samples.
Press
Esc
to return to the Protein Screen.
Press
to display available Options which are described below.
Options (select using keypad numbers)
1. Return to Parameters Screen (step 1 above).
2. Print result via selected method.
3. Toggle Graph on/off. Displays the calibration graph, cursors give values for last
measured sample.
7. Sample Number – add a prefix to the sample number and reset the
incrementing number to the desired value.
8. Save Method – use the alpha-numeric keys to enter a name for the method and
press Save
.
